Transgenic Plants with Over-expressed Cysteine Synthase for Environmental Resistance of Sulfur
Transgenic Plants with Over-expressed Cysteine Synthase for Environmental Resistance of Sulfur
批准号:
06557126
负责人:
SAITO Kazuki
金额:
$2.37万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995
中文摘要
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英文摘要
Cysteine biosynthesis is the major assimilation mechanism of sulfur in plants. Cysteine synthase (CSase) [O-acetylserine (thiol) -lyase] responsible for the terminal step of cysteine biosynthesis catalyzes the formation of L-cysteine from O-acetyl-L-serine (OAS) and hydrogen sulfide. OAS is supplied by serine acetyltransferase (SATase) from acetyl-CoA and serine. There are at least three isoforms of CSases in the plant cells, which are localized in cytoplasm, chloroplasts and mitochondria, respectively. The subcellular localization of these isoforms was confirmed by in vivo experiments using transgenic plants expressing the transit peptide-gus fusion genes. Transgenic tobacco plants, integrated with the constructs for 35S-CSaseA cDNA,35S-pearbcS transit peptide-CSaseA cDNA and 35S-antisense CSaseA cDNA,were obtained and analyzed for modulation of cysteine biosynthesis in responese to various sulfur stress. The results indicated that over-accumulated foreign cysteine synthase in chloroplasts could enhance biosynthetic flow of cysteine.
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K.Saito他: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J. Biol. Chem.270. 10321-16326 (1995)
K. Saito 等人:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征”,J. Biol. 10321-16326。
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K.Saito,他: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J.Biol.Chem.270. 16321-16326 (1995)
K. Saito 等人:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征。”J.Biol.Chem.270(1995)。
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通讯作者:
Saito K,Yokoyama H,Noji M,Murakoshi I: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J.Biol. Chem. 270. 16321-16326 (1995)
Saito K、Yokoyama H、Noji M、Murakoshi I:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征。”
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Saito K,Kanda R,Kurosawa M,Murakosi I: "Overexpression of a plant cysteine synthase gene and biosynthesis of a plant specific metabolite, b- (pyrazol-1-yl) -L-alanine, in Escherichia coli." Can. J.Chem.72. 188-192 (1994)
Saito K、Kanda R、Kurosawa M、Murakosi I:“在大肠杆菌中植物半胱氨酸合酶基因的过度表达和植物特异性代谢物 b-(吡唑-1-基)-L-丙氨酸的生物合成。”
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K.Saito 他: "Modulation of cystein biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine (thiol)-lyase]." Plant physiol.106. 887-895 (1994)
K. Saito 等人:“过度表达半胱氨酸合酶 [O-乙酰丝氨酸(硫醇)-裂解酶] 的转基因烟草叶绿体中半胱氨酸生物合成的调节。植物生理学 887-895(1994)”
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Cloning and expression of genes responsible for secondary metabolism in medicinal plants.
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