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Transgenic Plants with Over-expressed Cysteine Synthase for Environmental Resistance of Sulfur

Transgenic Plants with Over-expressed Cysteine Synthase for Environmental Resistance of Sulfur
具有过表达半胱氨酸合酶的转基因植物以抵抗硫环境
批准号:
06557126
负责人:
SAITO Kazuki
金额:
$2.37万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995

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中文摘要
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英文摘要
Cysteine biosynthesis is the major assimilation mechanism of sulfur in plants. Cysteine synthase (CSase) [O-acetylserine (thiol) -lyase] responsible for the terminal step of cysteine biosynthesis catalyzes the formation of L-cysteine from O-acetyl-L-serine (OAS) and hydrogen sulfide. OAS is supplied by serine acetyltransferase (SATase) from acetyl-CoA and serine. There are at least three isoforms of CSases in the plant cells, which are localized in cytoplasm, chloroplasts and mitochondria, respectively. The subcellular localization of these isoforms was confirmed by in vivo experiments using transgenic plants expressing the transit peptide-gus fusion genes. Transgenic tobacco plants, integrated with the constructs for 35S-CSaseA cDNA,35S-pearbcS transit peptide-CSaseA cDNA and 35S-antisense CSaseA cDNA,were obtained and analyzed for modulation of cysteine biosynthesis in responese to various sulfur stress. The results indicated that over-accumulated foreign cysteine synthase in chloroplasts could enhance biosynthetic flow of cysteine.
期刊论文(11)
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会议论文
K.Saito他: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J. Biol. Chem.270. 10321-16326 (1995)
K. Saito 等人:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征”,J. Biol. 10321-16326。
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通讯作者:
K.Saito,他: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J.Biol.Chem.270. 16321-16326 (1995)
K. Saito 等人:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征。”J.Biol.Chem.270(1995)。
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通讯作者:
Saito K,Yokoyama H,Noji M,Murakoshi I: "Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory roles in cysteine biosynthesis from watermelon." J.Biol. Chem. 270. 16321-16326 (1995)
Saito K、Yokoyama H、Noji M、Murakoshi I:“在西瓜半胱氨酸生物合成中发挥调节作用的植物丝氨酸乙酰转移酶的分子克隆和表征。”
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通讯作者:
Saito K,Kanda R,Kurosawa M,Murakosi I: "Overexpression of a plant cysteine synthase gene and biosynthesis of a plant specific metabolite, b- (pyrazol-1-yl) -L-alanine, in Escherichia coli." Can. J.Chem.72. 188-192 (1994)
Saito K、Kanda R、Kurosawa M、Murakosi I:“在大肠杆菌中植物半胱氨酸合酶基因的过度表达和植物特异性代谢物 b-(吡唑-1-基)-L-丙氨酸的生物合成。”
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11
    Establishment of molecular basis on ligand recognition by the insect prothoracicotropic hormone receptor
    Systematic structure optimization of the EGF receptor-inhibiting peptide through use of a screening technology
    • 批准号:
      24590134
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.49万
    • 财政年份:
      2012
    • 负责人:
      SAITO Kazuki
    • 依托单位:
    Integrated analysis of transcriptome and metabolome in Japanese medicinal plants
    • 批准号:
      24390030
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.65万
    • 财政年份:
      2012
    • 负责人:
      SAITO Kazuki
    • 依托单位:
    Development of peptide inhibitors against the EGF receptor based on its structural properties.
    海外基金