Elucidation of Adaptive Multimode Lubrication Mechanism, Self-Organization and Restoration Mechanism in Natural Synovial Joints

阐明自然滑膜关节的自适应多模式润滑机制、自组织和恢复机制

• 批准号: 15200037
• 负责人: MURAKAMI Teruo
• 金额: $ 27.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15200037/
• 关键词: Natural Synovial Joints; Adaptive Multimode Lubrication; Self-Organization; Articular Cartilage; Chondrocyte; Biomechanics; Biotribology

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints in relation to the self-organization and restoration from the hierarchic and microscopic viewpoints, to prevent the progress of osteoarthritis and to improve the function in regenerated cartilage.The atomic force microscopy (AFM) was applied to observe the morphological changes in articular cartilage surfaces removed from knee joints with osteoarthritis and porcine cartilage at different friction levels during rubbing experiments. The corresponding force-distance curves were examined to estimate the mechanical properties. The confocal laser scanning microscopy (CLSM) was used to estimate the local strain of articular cartilage under compression by observing the fluorescent images of stained chondrocytes. The morphology and distribution of chondrocytes in articular cartilage were investigated by CLSM. Three-dimensional numerical analysis based on biphasic finite element me … More thod was conducted to examine the influence of compressive stimulation on chondrocyte in articular cartilage. Lubricating gel film composed of proteoglycan appears to protect the bulk cartilage and maintain low friction even under severe rubbing. The proteoglycan in gel film is supplied through production by chondrocyte. The changes in stress-strain around chondrocyte was examined, because appropriate stimulation to chondrocyte can give an influence on the production of proteoglycan. Another protective mechanism with low friction is given by adsorbed film formation on the rubbing surface. The tribological property of polyvinyl-alcohol (PVA) hydrogel specimen as artificial cartilage or a model of cartilage was examined in hyaluronate solution containing globular serum proteins. The appropriate constituents composed of albumin and γ-globulin resulted in lower wear and lower friction. The difference in adsorptional behavior was clearly confirmed with in situ observation of fluorescent images of proteins. The total internal reflection fluorescence microscopy (TIRFM) was successfully applied to observe adsorptional behavior of proteins. Less

本研究的目的是从层次和微观角度阐明天然滑膜关节自组织和自修复的自适应多模润滑机制，以防止骨关节炎的进展和改善再生软骨的功能。采用原子力显微镜（AFM）观察了不同摩擦水平下骨性关节炎膝关节和猪软骨关节软骨表面的形态变化。测试了相应的力-距离曲线，以估计其力学性能。采用共聚焦激光扫描显微镜（CLSM），通过观察染色软骨细胞的荧光图像来估计关节软骨受压时的局部应变。用CLSM观察关节软骨内软骨细胞的形态和分布。采用双相有限元法进行三维数值分析，研究了压缩刺激对关节软骨软骨细胞的影响。由蛋白聚糖组成的润滑凝胶膜似乎可以保护大块软骨，即使在严重摩擦下也能保持低摩擦。凝胶膜中的蛋白多糖是由软骨细胞产生的。研究了软骨细胞周围应力应变的变化，因为对软骨细胞进行适当的刺激可以影响蛋白多糖的产生。另一种低摩擦的保护机制是在摩擦表面形成吸附膜。在含球状血清蛋白的透明质酸溶液中，研究了聚乙烯醇（PVA）水凝胶标本作为人工软骨或软骨模型的摩擦学性能。由白蛋白和γ-球蛋白组成的适当成分降低了磨损和摩擦。通过对蛋白质的荧光图像的原位观察，可以清楚地证实吸附行为的差异。利用全内反射荧光显微镜（TIRFM）成功地观察了蛋白质的吸附行为。少

项目成果

Importance of Time-dependent Deformation of Articular Cartilage Including Chondrocytes in Surface Zone

关节软骨（包括表面区软骨细胞）随时间变化的重要性

• 发表时间: 2005
• 期刊: Proc.the 2nd Asian Pacific Conference on Biomechanics (CDROM)
• 作者: Nobuo Kawaguchi;Shigeki Matsubara;Kazuya Takeda;Fumitada Itakura;K.Kumakura;Teruo MURAKAMI et al.
• 通讯作者: Teruo MURAKAMI et al.

Activated Wear by Lubricant Constituents in Boundary Lubrication and Protective Role of Boundary Lubrication in Joint Prostheses

边界润滑中润滑剂成分的活化磨损以及边界润滑对关节假体的保护作用

• 发表时间: 2004
• 期刊: Proc.7th World Congress of Biomaterials (CDROM)
• 作者: K.Yamamoto;et al.;Teruo MURAKAMI et al.
• 通讯作者: Teruo MURAKAMI et al.

Teruo MURAKAMI et al.: "The Protective Mechanism of Articular Cartilage to Severe Loading : Roles of Lubricants, Cartilage Surface Layer, Extracellular Matrix and Chondrocyte"JSME International Journal, Ser.C,. 46-2. 594-603 (2003)

Teruo MURAKAMI 等：“关节软骨对严重负荷的保护机制：润滑剂、软骨表面层、细胞外基质和软骨细胞的作用”JSME 国际期刊，Ser.C，。

力学物性評価に基づく生体組織のリモデリング機構の解明と人工材料適合性評価に基づく高機能人工関節の開発

基于力学性能评价阐明生物组织重塑机制，基于人工材料相容性评价开发高性能人工关节

• 发表时间: 2005
• 期刊: 福岡医学雑誌 第96号
• 作者: Teruo MURAKAMI et al.

人工軟骨候補材料ポリビニルアルコールハイドロゲルの摩耗評価及び耐摩耗性向上に寄与する蛋白質の影響

人工软骨候选材料聚乙烯醇水凝胶的磨损评估以及有助于提高耐磨性的蛋白质的影响

• 发表时间: 2004
• 期刊: 日本機械学会論文集(C編) 70-697
• 作者: T.Akahori;M.Niinomi;M.Nakai;H.Fukuda;H.Fukui;M.Ogawa;中嶋和弘 ほか
• 通讯作者: 中嶋和弘 ほか