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Micro- and Nano-Scopic Biomechanics of Articular Cartilage Focusing Chondrocytes and Surrounding Tissue

关节软骨的微观和纳米生物力学聚焦软骨细胞和周围组织

基本信息

批准号：
15086212
负责人：
MURAKAMI Teruo
金额：
$ 21.82万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15086212/
关键词：
Articular cartilage Micro-nano-biomechanics Chondrocyte Extracellular matrix Mechanical stimulus

项目摘要

The increase of elder persons disabled with walking ability due to osteoarthritis, rheumatoid arthritis or damages of natural synovial joints is anticipated with progress of population of aged people particularly in Japan. In natural synovial joint, the articular cartilage plays important roles in load-carrying mechanism and lubrication mechanism. The initiation of osteoarthritis is considered to be brought about by the deficiency of lubricating ability in synovial joints and loss of load-carrying capacity of articular cartilage. The articular cartilage is composed of extracellular matrix (ECM) and chondrocyte. The object of this research is to elucidate the stress-strain behaviors of articular cartilage including chondrocytes and the adaptive multimode lubrication mechanism in natural synovial joints from the macro-, micro- and nanoscopic viewpoints, to prevent the progress of osteoarthritis and to improve the function in regenerated cartilage.The confocal laser scanning microscopy (C … More LSM) was used to estimate the local strain of articular cartilage under compression by observing the fluorescent images of stained chondrocytes. The time-dependent and depth-dependent strain behaviors of articular cartilage were investigated by CLSM. These mechanical behaviors were evaluated by the biphasic finite element method (FEM). These stress-strain behaviors have an influence on metabolism controlled by chondrocytes which is expected to produce surrounding ECM including proteoglycan and collagen. The depth-dependent profile deformation and time-dependent strain behavior of semi-cylindrical cartilage specimen was simulated by biphasic finite element analyses considering depth-depending elastic modulus. The correspondence to the peak compressive stress and following stress relaxation immediately after compression at high strain rate was attained by consideration of one order raising of elastic modulus or by introducing of spring element like collagen fiber to biphasic model. Furthermore, the effect of cyclic compressive stimulation on cultured chondrocytes was indicated by an increase in elastic modulus of chondrocyte-agarose construct. Less

随着老年人口的增加，特别是在日本，由于骨关节炎、风湿性关节炎或天然滑膜关节损伤而丧失行走能力的老年人预计会增加。在天然滑膜关节中，关节软骨在承载机制和润滑机制中起着重要作用。骨关节炎的发生被认为是由于滑膜关节的润滑能力不足和关节软骨的承载能力丧失引起的。关节软骨由细胞外基质（ECM）和软骨细胞组成。本研究旨在从宏观、微观和纳米三个角度阐明天然滑膜关节中包括软骨细胞在内的关节软骨的应力-应变行为和自适应多模态润滑机制，以预防骨关节炎的发展，改善再生软骨的功能。采用共聚焦激光扫描显微镜（C…More LSM），通过观察染色软骨细胞的荧光图像来估计关节软骨受压时的局部应变。利用CLSM研究了关节软骨应变的时间依赖性和深度依赖性。采用双相有限元法对其力学性能进行了分析。这些应力-应变行为对软骨细胞控制的代谢有影响，软骨细胞有望在周围产生包括蛋白多糖和胶原蛋白在内的ECM。采用考虑弹性模量的双相有限元方法模拟了半圆柱形软骨试件的随深度轮廓变形和随时间应变行为。考虑弹性模量提高一个数量级或在双相模型中引入胶原纤维等弹簧元件，得到了高应变速率下压缩后的峰值压应力和随后的应力松弛的对应关系。此外，循环压缩刺激对培养软骨细胞的影响表明，软骨细胞琼脂糖结构的弹性模量增加。少