Micro- and Nano-Scopic Biomechanics of Articular Cartilage Focusing Chondrocytes and Surrounding Tissue

关节软骨的微观和纳米生物力学聚焦软骨细胞和周围组织

基本信息

  • 批准号:
    15086212
  • 负责人:
  • 金额:
    $ 21.82万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2006
  • 项目状态:
    已结题

项目摘要

The increase of elder persons disabled with walking ability due to osteoarthritis, rheumatoid arthritis or damages of natural synovial joints is anticipated with progress of population of aged people particularly in Japan. In natural synovial joint, the articular cartilage plays important roles in load-carrying mechanism and lubrication mechanism. The initiation of osteoarthritis is considered to be brought about by the deficiency of lubricating ability in synovial joints and loss of load-carrying capacity of articular cartilage. The articular cartilage is composed of extracellular matrix (ECM) and chondrocyte. The object of this research is to elucidate the stress-strain behaviors of articular cartilage including chondrocytes and the adaptive multimode lubrication mechanism in natural synovial joints from the macro-, micro- and nanoscopic viewpoints, to prevent the progress of osteoarthritis and to improve the function in regenerated cartilage.The confocal laser scanning microscopy (C … More LSM) was used to estimate the local strain of articular cartilage under compression by observing the fluorescent images of stained chondrocytes. The time-dependent and depth-dependent strain behaviors of articular cartilage were investigated by CLSM. These mechanical behaviors were evaluated by the biphasic finite element method (FEM). These stress-strain behaviors have an influence on metabolism controlled by chondrocytes which is expected to produce surrounding ECM including proteoglycan and collagen. The depth-dependent profile deformation and time-dependent strain behavior of semi-cylindrical cartilage specimen was simulated by biphasic finite element analyses considering depth-depending elastic modulus. The correspondence to the peak compressive stress and following stress relaxation immediately after compression at high strain rate was attained by consideration of one order raising of elastic modulus or by introducing of spring element like collagen fiber to biphasic model. Furthermore, the effect of cyclic compressive stimulation on cultured chondrocytes was indicated by an increase in elastic modulus of chondrocyte-agarose construct. Less
随着老年人口的发展,特别是在日本,由于骨关节炎、类风湿性关节炎或自然滑膜关节损伤而导致行走能力残疾的老年人预计会增加。在天然滑膜关节中,关节软骨在承载机制和润滑机制中起着重要作用。骨关节炎的发生被认为是由于滑膜关节润滑能力不足和关节软骨承载能力丧失所致。关节软骨由细胞外基质(ECM)和软骨细胞组成。本研究旨在从宏观、微观和纳米三个角度阐明关节软骨(包括软骨细胞)的应力-应变行为和自然滑膜关节的适应性多模式润滑机制,以预防骨关节炎的进展和改善再生软骨的功能。 ...更多信息 LSM)通过观察染色软骨细胞的荧光图像来估计关节软骨在压缩下的局部应变。采用激光共聚焦扫描电镜研究了关节软骨的应变行为随时间和深度的变化。这些力学行为进行了评估,通过双相有限元法(FEM)。这些应力-应变行为对由软骨细胞控制的代谢具有影响,预期软骨细胞产生周围ECM,包括蛋白聚糖和胶原。采用弹性模量随深度变化的双相有限元方法,模拟了半圆柱形软骨试件随深度变化的剖面变形和随时间变化的应变行为。通过考虑弹性模量的一阶增长或在两相模型中引入胶原纤维等弹性元件,得到了高应变率下压缩后峰值压缩应力与随后的应力松弛的对应关系。此外,软骨细胞-琼脂糖构建体的弹性模量增加表明循环压缩刺激对培养的软骨细胞的影响。少

项目成果

期刊论文数量(75)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Time-dependent and Depth-dependent Deformation of Biphasic Articular Cartilage under Constant Total Compressive Deflection
恒定总压缩变形下双相关节软骨的时间依赖性和深度依赖性变形
人工軟骨候補材料ポリビニルアルコールハイドロゲルの摩耗評価及び耐摩耗性向上に寄与する蛋白質の影響
人工软骨候选材料聚乙烯醇水凝胶的磨损评估以及有助于提高耐磨性的蛋白质的影响
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Akahori;M.Niinomi;M.Nakai;H.Fukuda;H.Fukui;M.Ogawa;中嶋和弘 ほか
  • 通讯作者:
    中嶋和弘 ほか
Interaction of Protein with Phospholipids Surface Layer Studied by Total Internal Reflection Fluorescence Microscopy
全内反射荧光显微镜研究蛋白质与磷脂表面层的相互作用
Time-dependent Compressive Deformation of Articular Cartilage and Chondrocytes
关节软骨和软骨细胞的时间依赖性压缩变形
生体工学概論
生物工程简介
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    村上輝夫(編著)
  • 通讯作者:
    村上輝夫(編著)
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MURAKAMI Teruo其他文献

MURAKAMI Teruo的其他文献

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{{ truncateString('MURAKAMI Teruo', 18)}}的其他基金

Elucidation of adaptive lubrication mechanism with low friction and minimum wear in natural synovial joints and development of artificial hydrogel cartilage with super lubricity based on bionic design
阐明自然滑膜关节中低摩擦和最小磨损的自适应润滑机制,并开发基于仿生设计的超润滑人工水凝胶软骨
  • 批准号:
    23000011
  • 财政年份:
    2011
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Development of reversing agent for multidrug resistance and new chemotherapy by utilizing genistein
利用金雀异黄素开发多药耐药逆转剂及新型化疗药物
  • 批准号:
    18590162
  • 财政年份:
    2006
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of Adaptive Multimode Lubrication Mechanism, Self-Organization and Restoration Mechanism in Natural Synovial Joints
阐明自然滑膜关节的自适应多模式润滑机制、自组织和恢复机制
  • 批准号:
    15200037
  • 财政年份:
    2003
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Elucidation of Adaptive Multimode Lubrication Mechanism and Self-Organization in Natural Synovial Joints
自然滑膜关节自适应多模式润滑机制和自组织的阐明
  • 批准号:
    12480265
  • 财政年份:
    2000
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of Optimum Design, Trial Production and Evaluation Systems for Knee Joint Prostheses with Artificial Cartilage and Meniscus Similar to Natural Synovial Joints
类似天然滑膜关节的人工软骨和半月板膝关节假体优化设计、试制及评价系统开发
  • 批准号:
    11358014
  • 财政年份:
    1999
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Effect of endocrine disrupting chemicals on host defense mechanism (transpoter, enzymes)
内分泌干​​扰化学物质对宿主防御机制(转运蛋白、酶)的影响
  • 批准号:
    11557200
  • 财政年份:
    1999
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Joint Study on the Development of Novel Joint Prostheses
新型关节假体开发联合研究
  • 批准号:
    10044165
  • 财政年份:
    1998
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Elucidation of Adaptive Multimode Lubrication Mechanism in Natural Synovial Joints from Hierarchic and Microscopic Viewpoints
从层次和微观角度阐明自然滑膜关节的自适应多模式润滑机制
  • 批准号:
    09480254
  • 财政年份:
    1997
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Lubrication Mechanism in Natural and Artificial Joints
自然关节和人工关节的润滑机制
  • 批准号:
    07044159
  • 财政年份:
    1995
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Elucidation of Adaptive Multimode Lubrication, Deterioration and Restoration in Natural Synovial Joints
自然滑膜关节自适应多模式润滑、恶化和恢复的阐明
  • 批准号:
    07458237
  • 财政年份:
    1995
  • 资助金额:
    $ 21.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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