Elucidation of Adaptive Multimode Lubrication Mechanism in Natural Synovial Joints from Hierarchic and Microscopic Viewpoints

本研究旨在从层次和微观两个角度阐明自然滑膜关节的自适应多模式润滑机制，对自然滑膜关节的自适应多模式润滑机制进行了系统的研究。膝关节模型在模拟器上的流体膜形成的实验评估和数值分析表明，弹性流体动力润滑在步行过程中的重要作用。在摆锤法猪肩关节摩擦试验中，阐明了透明质酸浓度对高低载荷条件下摩擦性能的影响与去除吸附膜、添加磷脂或蛋白质有关。由磷脂、蛋白质和糖蛋白组成的吸附膜的边界润滑清楚地显示为去除吸附膜后摩擦的增加。添加磷脂脂质体和丙种球蛋白可有效降低摩擦系数 关于我们 对关节软骨滑动对与玻璃板上形成的Langmuir-Blodgett膜进行往复测试，表明磷脂和蛋白质混合膜优于磷脂膜。在体内试验中证实了混合吸附膜的保护作用。应用原子力显微镜（AFM）观察不同摩擦力水平下关节软骨摩擦过程中表面的变化。在几个摩擦水平相应的力-距离曲线进行了检查。结果表明，随着磨损过程的进行，吸附膜和凝胶膜的润滑作用发生了变化。比较了聚乙烯醇（PVA）水凝胶人工软骨和天然关节软骨的摩擦学性能，以阐明两者之间的差异。采用锥板粘度计对单层培养软骨细胞进行连续剪切实验，观察剪切刺激对关节软骨细胞的影响，并通过三维数值分析研究剪切刺激对关节软骨细胞白细胞介素6（IL-6）水平的影响。少

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints from the hierarchic and microscopic viewpoints.The systematic investigation was performed to clarify the adaptive multimode lubrication mechanism in natural synovial joints. Experimental evaluation of fluid film formation in knee joint model in simulator and numerical analysis showed the important role of elastohydrodynamic lubrication during walking. In friction tests of pig shoulder joints by pendulum method, the influence of hyaluronate concentration on frictional behavior under high and low load conditions was clarified in related to removal of adsorbed films or addition of phospholipid or proteins. The boundary lubrication by adsorbed film composed of phospholipids, proteins and glycoproteins was clearly shown by an increase in friction after removal of adsorbed film. Addition of liposome of phospholipid liposome and gamma-globulin was effective in lowering of fricti … More on. Reciprocating tests of sliding pair of articular cartilage and Langmuir-Blodgett film formed on glass plate indicated the superiority of mixed film of phospholipid and protein to phospholipid film. The protecteive effect by mixed adsorbed film was confirmed in in-vivo tests. The atomic force microscopy (AFM) was applied to observe the changes in articular cartilage surfaces at different friction levels during rubbing process. The corresponding force-distance curves at several friction levels were examined. These results indicated that lubricating roles of adsorbed film and gel film changes with progressive wear process. The tribological property of polyvinyl-alcohol (PVA) hydrogel specimen as artificial cartilage was compared with that of natural articular cartilage to clarify the difference between both materials. The three-dimensional numerical analysis was planned to examine the influence of shearing stimulation on chondrocyte in articular cartilage which was found in continuous shearing test to monolayer of cultured chondrocyte in cone/plate viscometer inducing the remarkable increase in interleukin 6 (IL-6). Less