Elucidation of Adaptive Multimode Lubrication Mechanism and Self-Organization in Natural Synovial Joints

自然滑膜关节自适应多模式润滑机制和自组织的阐明

• 批准号: 12480265
• 负责人: MURAKAMI Teruo
• 金额: $ 9.47万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480265/
• 关键词: Natural Synovial Joints; Adaptive Multimode Lubrication; Self-Organization; Cartilage; Chondrocyte; Artificial Cartilage; Adsorbed Film; Biotribology

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints in relation to the self-organization from the hierarchic and microscopic viewpoints.The systematic investigation was performed to clarify the adaptive multimode lubrication mechanism in natural synovial joints. Experimental evaluation of fluid film formation in knee joint model in simulator and numerical analysis showed the important role of elastohydrodynamic lubrication during walking. In friction tests of pig shoulder joints by pendulum method, the influence of hyaluronate concentration on frictional behavior under high and low load conditions was clarified in related to removal of adsorbed films or addition of phospholipid or proteins. The boundary lubrication by adsorbed film composed of phospholipids, proteins and glycoproteins was clearly shown by an increase in friction after removal of adsorbed film. Addition of phospholipid liposome and γ-globulin was effective … More in lowering of friction. The atomic force microscopy (AFM) was applied to observe the changes in articular cartilage surfaces at different friction levels during rubbing process. The corresponding force-distance curves at several friction levels were examined. These results indicated that lubricating roles of adsorbed film and gel film change with progressive wear process. The tribological property of polyvinyl-alcohol (PVA) hydrogel specimen as artificial cartilage was compared with that of natural articular cartilage to clarify the difference between both materials. The three-dimensional numerical analysis based on finite element method was conducted to examine the influence of compressive stimulation on chondrocyte in articular cartilage. The morphology and distribution of chondrocytes in articular cartilage were investigated by confocal laser scanning microscopy (CLSM) to construct the three-dimensional model. It was noticed that the local strain near surface had larger value than average one immediately after compressive loading and recovered to the average level. This local high strain has the possibility to have a remarkable effect on the chondrocyte located in those zones. The changes in strain were observed by CLSM. Less

本研究旨在从层次和微观的角度阐明自然滑膜关节自组织的自适应多模式润滑机制，对自然滑膜关节自适应多模式润滑机制进行了系统的研究。膝关节模型在模拟器上的流体膜形成的实验评估和数值分析表明，弹性流体动力润滑在步行过程中的重要作用。在摆锤法猪肩关节摩擦试验中，阐明了透明质酸浓度对高低载荷条件下摩擦性能的影响与去除吸附膜、添加磷脂或蛋白质有关。由磷脂、蛋白质和糖蛋白组成的吸附膜的边界润滑清楚地显示为去除吸附膜后摩擦的增加。添加磷脂脂质体和γ-球蛋白是有效的 ...更多信息 降低摩擦。应用原子力显微镜（AFM）观察不同摩擦力水平下关节软骨摩擦过程中表面的变化。在几个摩擦水平相应的力-距离曲线进行了检查。结果表明，随着磨损过程的进行，吸附膜和凝胶膜的润滑作用发生了变化。比较了聚乙烯醇（PVA）水凝胶人工软骨和天然关节软骨的摩擦学性能，以阐明两者之间的差异。采用三维有限元数值分析方法，研究了压力刺激对关节软骨细胞的影响。采用激光共聚焦扫描显微镜（CLSM）观察关节软骨细胞的形态和分布，构建三维模型。结果表明，在压缩载荷作用下，表层局部应变值大于平均应变值，但在压缩载荷作用下，表层局部应变恢复到平均水平。这种局部高应变有可能对位于这些区域的软骨细胞产生显著影响。CLSM法观察菌株的变化。少

项目成果

K.Nakashima: "Evaluation of Tribological Properties of Polyvinyl Alcohol Hydrogel as Artificial Cartilage"Proc. International Tribology conference Nagasaki, 2000. 1537-1540 (2001)

K.Nakashima：“聚乙烯醇水凝胶作为人工软骨的摩擦学特性的评估”Proc。

S.Moriyama: "Influence of Motional Conditions on Fluid Film Formation in Knee Prostheses"Proc. International Tribology conference Nagasaki, 2000. 1515-1520 (2001)

S.Moriyama：“运动条件对膝关节假体液膜形成的影响”Proc。

T.Murakami: "Importance of lubrication modes in natural synovial joints, joint prostheses and human skin"Proc. Biotribology Satellite Forum of ITC Nagasaki, 2000 and 21st Biotribology Symposium. 1-4 (2000)

T.Murakami：“润滑模式在自然滑膜关节、关节假体和人体皮肤中的重要性”Proc。

T.Murakami: "Biotribological Aspects of Natural Synovial Joints and Artificial Joints"Human Biomechanics and Injury Prevention. 234-242 (2000)

T.Murakami：“自然滑膜关节和人工关节的生物摩擦学方面”人体生物力学和损伤预防。

T. Murakami: "Tribological Behavior of Artificial Cartilage in Thin Film Lubrication"Thinning Films and Tribological Interface. 317-327 (2000)

T. Murakami：“薄膜润滑中人工软骨的摩擦学行为”薄膜和摩擦学界面。