Applying ferrite nanobeads to medical diagnosis, therapy and analysis

将铁氧体纳米珠应用于医学诊断、治疗和分析

• 批准号: 15209019
• 负责人: ABE Masanori
• 金额: $ 32.45万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15209019/
• 关键词: Magnetic beads; Bio-screening; Bio-sensing; MRI contrast agent; Hyperthermia; Anticancer drug; DNA; Ferrite nanobeads; 磁性ビーズ; DNA検出; MRI造影剤; フェライト粒子; GMA; 抗体; MMI造影; フェライトナノビーズ; IgG抗体; CEA抗体

1. Development of size controlled ferrite nanobeadsIron-ferrite nanoparticles with controlled size of 10-100 nm were successfully synthesized by our newly developed methods called co-precipitation, partial-oxidation, and seed-growth methods.2. Development of functional-polymer coated ferrite nanobeadsThe iron-ferrite nanoparticles were successfully coated with poly-GMA, which exhibits minimal non-specific adsorption of proteins, by a co-polymerization method and a novel, soap-free polymerization method.3. Development of polymer coated iron nanobeadsMetallic iron nanoparticles (7-20 nm in size), which have magnetization two times stronger than that of ferrite particles and are advantageous for magnetic separation in bio-screening, were successfully coated with poly-GMA.4. Application to analysis by bio-screening systemThe poly-GMA coated ferrite nanobeads were conjugated with physiologically active substances such as anticancer antibodies and recombinant proteins containing tagged Prote … More in-G. Utilizing the beads, we successfully captured physiologically active substances that are complementary to those conjugated onto the beads. This revealed our beads are fitted for magnetic carriers for high throughput bio-screening.5. Application to diagnosis by Hall bio-sensingpoly-GMA coated ferrite nanobeads were conjugated with single strand DNA, which were immobilized onto the surface of a Hall sensor via hybridization with the complementary DNA single strand fixed on the sensor surface. This revealed our beads are applicable to magnetic carriers for DNA analysis.6. Application to diagnosis by contrast enhanced MRIWe successfully modified the surfaces of the ferrite nanobeads with a variety of functional molecules, and discovered candidate materials for high performance MRI contrast agent.7. Application to anticancer magnetic hyperthermiaUtilizing the ferrite nanobeads as heat source by magnetic-field induction at an non-invasive frequency of 120 kHz, we successfully killed human colon cancer cells. We thus revealed that our beads are applicable to magnetic carriers for non-invasive, anticancer magnetic hyperthermia. Less

1.尺寸可控的铁氧体纳米粒子的制备采用我们新开发的共沉淀法、部分氧化法和晶种生长法成功地合成了尺寸可控的10-100 nm的铁铁氧体纳米粒子.功能性聚合物包覆铁氧体纳米微球的研制通过共聚合法和一种新型的无皂聚合法，成功地将对蛋白质非特异性吸附最小的聚-GMA包覆在铁-铁氧体纳米粒子上.聚合物包覆铁纳米微球的研制成功地将聚甲基丙烯酸缩水甘油酯（poly-GMA）包覆在金属铁纳米粒子（粒径为7-20 nm）上，这种铁纳米粒子的磁化强度是铁氧体粒子的2倍，有利于生物筛选中的磁性分离。在生物筛选系统分析中的应用聚-GMA包覆的铁氧体纳米珠与生理活性物质如抗癌抗体和含有标记蛋白的重组蛋白缀合。 ...更多信息 在G中。利用珠子，我们成功地捕获了与缀合到珠子上的物质互补的生理活性物质。这表明我们的磁珠适合作为高通量生物筛选的磁性载体。应用于霍尔传感器的诊断将聚-GMA包覆的铁氧体纳米珠与单链DNA偶联，通过与固定在霍尔传感器表面的互补DNA单链杂交将其固定在霍尔传感器表面。这表明我们的微球适用于DNA分析的磁性载体。应用于磁共振造影诊断我们成功地用多种功能分子修饰了铁氧体纳米珠的表面，发现了高性能磁共振造影剂的候选材料.应用于抗癌磁性高渗利用铁氧体纳米珠作为热源，通过非侵入性频率为120 kHz的磁场诱导，我们成功地杀死了人结肠癌细胞。因此，我们发现，我们的珠适用于非侵入性，抗癌磁热疗的磁性载体。少

项目成果

The VP2/VP3 minor capsid ptotein of SV40 promotes the in vitro assembly of the major capsid protein VP1 into particles

SV40的VP2/VP3次要衣壳蛋白促进主要衣壳蛋白VP1在体外组装成颗粒

• 发表时间: 2006
• 期刊: J. Biol. Chem 281
• 作者: Kawano;M.;Inoue;T.;Tsukamoto;H.;Takaya;T.;Enomoto;T.;Takahashi;R. U.;Yokoyama;N.;Yamamoto;N.;Nakanishi;A.;Imai;T.;Wada;T.;Kataoka;K. and Handa;H
• 通讯作者: H

磁性ビーズのバイオ・環境技術への応用展開

磁珠在生物/环境技术中的应用

• 发表时间: 2006
• 作者: Takahashi;A.;et. al.;B.Jeyadevan
• 通讯作者: B.Jeyadevan

A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers.

• 发表时间: 2003-07
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: T. Ezaki;Mamoru Watanabe;N. Inoue;T. Kanai;H. Ogata;Y. Iwao;H. Ishii;T. Hibi

H.Nohara: "Association between the interleukin-1 receptor antagonist polymorphism and ulcerative colitis with younger age at diagnosis"Immunol Lett. 90・1. 53-57 (2003)

H.野原：“白细胞介素1受体拮抗剂多态性与诊断时年龄较小的溃疡性结肠炎之间的关联”Immunol Lett 90・1（2003）。

The VP2/VP3 minor capsid plotein of SV40 promotes the in vitroassembly of the major capsid protein VPl into particles.

SV40的VP2/VP3次要衣壳蛋白促进主要衣壳蛋白VP1在体外组装成颗粒。

• 期刊: J.Biol.Chem. in press
• 作者: Kawano;M.;Inoue;T.;Tsukamoto;H.;Takaya;T.;Enomoto;T.;Takahashi;R.U.;Yokoyama;N.;Yamamoto;N.;Nakanishi;A.;Imai;T.;Wada;T.;Kataoka;K.;Handa;H.
• 通讯作者: H.