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Control of Nano-Reaction Field by Bio-Probe Nanolithography

生物探针纳米光刻控制纳米反应场

基本信息

批准号：
16206072
负责人：
TAKAI Osamu
金额：
$ 31.87万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

Construction of the molecular device is one of the biggest aims in nanotechnology. Nanolithography is essential to the formulation of such a molecular device. We have focused on Scanning Probe Lithography (SPL) as one of such nanolithographic techniques. In this study, we attempted to immobilize biomolecules such as proteins in nano-domains by SPL.At first, we have prepared surfaces with some surface characteristics by fabrication of self-ascembled monolayer (SAM) and investigated adsorption behaviors of biomolecules such as proteins on the SAMs. As a result, it was clarified that the surface wetting and surface potential, chemical affinity had great influences on the adsorption behavior of the biomolecules.Next, we have fabricated micropatterned surfaces with two different domains in the surface characteristics(surface wetting, surface potential, chemical affinity)by light lithography and observed adsorption behavior of biomolecules on them. Then it was confirmed that the adsorbent adhered to the specific domain of the micro-pattern. We successfully controlled the immobilization of the adsorbents on the specific surface by the surface characteristics mentioned above.Finally we have manufactured nanopatterned surfaces with different surface characteristics by SPL. Gold nanostructures on a silicon substrate were constructed by "Dip-Pen Nanolithography(DPN)"as one of SPL. Then, various kinds of frijol compounds were adsorbed on the structure body and surface characteristics of the nano-domain surface were controlled. We have successfully achieved position-selective immobilization of nanoparticles, as a model compound of a protein, and DNA.

分子器件的构建是纳米技术的最大目标之一。纳米光刻对于这种分子器件的设计至关重要。我们重点关注扫描探针光刻（SPL）作为此类纳米光刻技术之一。在本研究中，我们尝试通过SPL将蛋白质等生物分子固定在纳米域中。首先，我们通过制造自组装单分子层（SAM）制备了具有一定表面特性的表面，并研究了蛋白质等生物分子在SAM上的吸附行为。结果表明，表面润湿、表面电位、化学亲和力对生物分子的吸附行为有很大影响。接下来，我们通过光刻技术制备了具有两个不同表面特征（表面润湿、表面电位、化学亲和力）域的微图案表面，并观察了生物分子在其上的吸附行为。然后证实吸附剂粘附到微图案的特定区域。通过上述表面特性，我们成功地控制了吸附剂在特定表面上的固定。最后，我们通过SPL制造了具有不同表面特性的纳米图案表面。采用SPL技术之一的“浸笔纳米光刻(DPN)”技术在硅衬底上构建了金纳米结构。然后，将各种氟荷化合物吸附在结构体上，并控制纳米域表面的表面特性。我们已经成功实现了纳米颗粒的位置选择性固定，作为蛋白质和 DNA 的模型化合物。