Control of Nano-Reaction Field by Bio-Probe Nanolithography

分子装置的构建是纳米技术的最大目标之一。纳米光刻技术对于这种分子器件的制作至关重要。扫描探针光刻（SPL）是纳米光刻技术中的一种。在这项研究中，我们试图通过SPL将生物分子（如蛋白质）固定在纳米结构域。首先，我们通过制备自组装单层膜（SAM）制备了具有某些表面特征的表面，并研究了生物分子（如蛋白质）在自组装单层膜上的吸附行为。结果表明，表面润湿性、表面电位、化学亲和力对生物分子的吸附行为有很大的影响。接下来，我们利用光刻技术制备了具有两种不同表面特征域（表面润湿性、表面电位、化学亲和性）的微图案化表面，并观察了生物分子在其上的吸附行为。然后证实吸附剂粘附在微图案的特定区域上。利用上述表面特性，我们成功地控制了吸附剂在特定表面上的固定。最后利用SPL制备了具有不同表面特性的纳米图案表面。采用浸笔纳米光刻技术（Dip-Pen Nanolithography， DPN）在硅衬底上构建了金纳米结构。然后，在结构体上吸附了多种戊醇类化合物，控制了纳米畴表面的表面特性。我们已经成功地实现了纳米颗粒的位置选择性固定化，作为蛋白质和DNA的模型化合物。

Construction of the molecular device is one of the biggest aims in nanotechnology. Nanolithography is essential to the formulation of such a molecular device. We have focused on Scanning Probe Lithography (SPL) as one of such nanolithographic techniques. In this study, we attempted to immobilize biomolecules such as proteins in nano-domains by SPL.At first, we have prepared surfaces with some surface characteristics by fabrication of self-ascembled monolayer (SAM) and investigated adsorption behaviors of biomolecules such as proteins on the SAMs. As a result, it was clarified that the surface wetting and surface potential, chemical affinity had great influences on the adsorption behavior of the biomolecules.Next, we have fabricated micropatterned surfaces with two different domains in the surface characteristics(surface wetting, surface potential, chemical affinity)by light lithography and observed adsorption behavior of biomolecules on them. Then it was confirmed that the adsorbent adhered to the specific domain of the micro-pattern. We successfully controlled the immobilization of the adsorbents on the specific surface by the surface characteristics mentioned above.Finally we have manufactured nanopatterned surfaces with different surface characteristics by SPL. Gold nanostructures on a silicon substrate were constructed by "Dip-Pen Nanolithography(DPN)"as one of SPL. Then, various kinds of frijol compounds were adsorbed on the structure body and surface characteristics of the nano-domain surface were controlled. We have successfully achieved position-selective immobilization of nanoparticles, as a model compound of a protein, and DNA.