Construction of Protein-RNA conjugate for intracellular imaging of metabolites

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In this study, construction of Protein-RNA conjugate, which enables detection of small molecules, was performed. RNA libraries that consist of artificial RNA aptamer and TAR-RNA were designed. RNAs that allosterically interact with Tat-peptide in the presence of the target small molecules were selected from the libraries. By cooperative function of artificially modified Luciferase containing Tat-peptide, the selected RNAs enabled luminometric detection of the small molecules. Additionally, direct conjugation of RNA aptamer and TAR-RNA enabled detection of small molecule by an intracellular gene expression though an allosteric interaction of Tat protein.