Function of p53 tumor-suppressor gene mediated autophagy

在这项研究中，我们的目标是确定新的p53靶基因调控自噬使用微阵列。首先，建立p53基因敲除细胞系和对照细胞系，并收集氨基酸饥饿后的细胞系。提取并纯化细胞总RNA。我们从总RNA中纯化cRNA，并进行cDNA微阵列。现在，我们正在评估自噬诱导过程中p53基因表达的差异。

In this study, our objective is to identify novel p53-target genes to regulate autophagy using microarray. First, we established the p53 knock-down and the control cell lines, and Each cell lines were collected after amino acid starvation. Total cellular RNA was extracted and purified. We purified cRNA from total RNA, and performed cDNA microarray. Now, we are evaluating the difference in gene expressions by the presense of p53 during induction of autophagy.