Function of p53 tumor-suppressor gene mediated autophagy

在这项研究中，我们的目标是利用微阵列识别新的p53靶基因来调节自噬。首先，我们建立p53敲除细胞系和对照细胞系，并在氨基酸饥饿后收集每个细胞系。提取并纯化细胞总RNA。从总RNA中纯化cRNA，并进行cDNA微阵列分析。现在，我们正在评估p53在诱导自噬过程中基因表达的差异。

In this study, our objective is to identify novel p53-target genes to regulate autophagy using microarray. First, we established the p53 knock-down and the control cell lines, and Each cell lines were collected after amino acid starvation. Total cellular RNA was extracted and purified. We purified cRNA from total RNA, and performed cDNA microarray. Now, we are evaluating the difference in gene expressions by the presense of p53 during induction of autophagy.