Carcinogenesis caused by tobacco and alcohol for human tongue keratinocytes (HTK) cell line

烟草和酒精对人舌角质形成细胞（HTK）细胞系的致癌作用

• 批准号: 21890287
• 负责人: SEGAWA Emi
• 金额: $ 1.56万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890287/
• 关键词: 口腔癌; HPV; 発癌

To elucidate the carcinogenesis process, we newly established human tongue keratinocytes (HTK) cell lines derived from tongue mucosa immortalized by infection with retroviral vectors expression HPV16 E6/E7. Furthermore, treatment of HPV16 E6/E7 with NNK and EtOH in vitro, and HTK1-16E6E7-NNK + EtOH was established. Then we examined a change of the cell biology in both cells and identified the responsibility gene in the carcinogenesis in oral squamous cell carcinoma.

为了阐明癌变过程，我们新建立了人舌角质形成细胞（HTK）细胞系，这些细胞系源自舌粘膜，这些舌粘膜因逆转录病毒载体表达HPV16 E6/E7而永生。此外，还建立了HPV16 E6/E7在体外用NNK和ETOH处理HTK1-16E7-NNK + ETOH。然后，我们检查了两个细胞中细胞生物学的变化，并确定了口服鳞状细胞癌的癌变中的责任基因。

项目成果

Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2

环氧合酶-2过表达促进人KB癌细胞的血行转移潜能

• 发表时间: 2010
• 期刊: Oncology Reports
• 影响因子: 4.2
• 作者: Segawa E;et al.
• 通讯作者: et al.

Inhibitory effect of sulindac on DMBA-induced hamster cheek pouch carcinogenesis and its derived cell line.

舒林酸对 DMBA 诱导的仓鼠颊囊癌及其衍生细胞系的抑制作用。

• 发表时间: 2009
• 期刊: Oncology Reports 21
• 作者: Segawa E.;Hashitami S.;etc
• 通讯作者: etc