Carcinogenesis caused by tobacco and alcohol for human tongue keratinocytes (HTK) cell line

为了阐明HPV16E6/E7的致癌过程，我们用表达HPV16E6/E7的逆转录病毒载体，建立了经感染永生化的舌粘膜来源的人舌角质形成细胞系。在此基础上，建立了NNK和EtoH对HPV16E6/E7的体外作用，以及HTK1-16E6E7-NNK+Etoh对HPV16E6/E7的作用。然后，我们检测了两种细胞的细胞生物学变化，并确定了口腔鳞状细胞癌发生中的责任基因。

To elucidate the carcinogenesis process, we newly established human tongue keratinocytes (HTK) cell lines derived from tongue mucosa immortalized by infection with retroviral vectors expression HPV16 E6/E7. Furthermore, treatment of HPV16 E6/E7 with NNK and EtOH in vitro, and HTK1-16E6E7-NNK + EtOH was established. Then we examined a change of the cell biology in both cells and identified the responsibility gene in the carcinogenesis in oral squamous cell carcinoma.