Carcinogenesis caused by tobacco and alcohol for human tongue keratinocytes (HTK) cell line

为了阐明其癌变过程，我们用表达HPV16 E6/E7的逆转录病毒载体感染人舌粘膜，建立了人舌角质形成细胞（HTK）细胞系。进一步建立了NNK和EtOH对HPV16 E6/E7的体外处理，以及HTK1-16E6E7-NNK + EtOH。然后我们检测了这两种细胞的细胞生物学变化，并确定了口腔鳞状细胞癌发生的责任基因。

To elucidate the carcinogenesis process, we newly established human tongue keratinocytes (HTK) cell lines derived from tongue mucosa immortalized by infection with retroviral vectors expression HPV16 E6/E7. Furthermore, treatment of HPV16 E6/E7 with NNK and EtOH in vitro, and HTK1-16E6E7-NNK + EtOH was established. Then we examined a change of the cell biology in both cells and identified the responsibility gene in the carcinogenesis in oral squamous cell carcinoma.