Real time kinetic analysis of the skin dendritic cells in the steady state and various stimulus conditions.

决定皮肤免疫反应多样性的因素在很大程度上仍是未知的。本研究的目的是阐明其在免疫反应中的作用和参与免疫反应的分子的识别，特别是针对外界刺激。我们使用Langerin-EGFP小鼠在稳态、相对弱的刺激如胶带等条件下观察了一段时间内皮肤树突状细胞的动态行为，并应用荧光标记的半抗原建立了体内实时成像方法。此外，我们还通过静脉内分化和培养的T细胞转移到Th1和Th2 T细胞(Egawa Et)后，评估了免疫细胞在皮肤中的动态和细胞迁移。艾尔。此外，我们已经证明，作为Rho家族的细胞骨架分子之一的mDia1，在皮肤树突状细胞的吞噬、迁移、黏附以及与其他免疫细胞如免疫突触的免疫突触形成中起着至关重要的作用。最后，我们报告了该系统(Tanizaki Et)失效时没有形成接触性皮炎反应。艾尔血液2010年12月)

The factors that determine the diversity of the immune response in the skin is still largely unknown. The purpose of this study was to elucidate its role and identification of molecules involved in the immune responses, especially against external stimuli.We observed the dynamics behavior of dendritic cells of the skin using a Langerin-eGFP mouse over a period of time in the steady state, a relatively weak stimulus such as tape strip, and applied fluorescence-labeled hapten and established the real-time imaging method in vivo.In addition, we have made it possible to evaluate the dynamics and cell migration of immune cells in the skin after that we transfer intravenously differentiated and cultured T cellsinto type Th1and Th2 T cells(Egawa et. Al. JID 2011 Apr).And more, we have shown that mDia1, which is one of the cytoskeletal molecules in the Rho family, play an essential role in the phagocytosis, migration, adhesion of skin dendritic cells, and immune synapse formation with the another immune cells, such as immunological synapse.Finally, we repoted that the contact dermatitis response is not formed at the time of the failure of this system(tanizaki et. al. Blood 2010 Dec)