Real time kinetic analysis of the skin dendritic cells in the steady state and various stimulus conditions.

皮肤树突状细胞在稳态和各种刺激条件下的实时动力学分析。

• 批准号: 22890093
• 负责人: TANIZAKI Hideaki
• 金额: $ 1.96万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890093/
• 关键词: 皮膚樹状細胞; ランゲルハンス細胞; mDia; 皮膚アレルギー; Langerin; 樹状細胞

The factors that determine the diversity of the immune response in the skin is still largely unknown. The purpose of this study was to elucidate its role and identification of molecules involved in the immune responses, especially against external stimuli.We observed the dynamics behavior of dendritic cells of the skin using a Langerin-eGFP mouse over a period of time in the steady state, a relatively weak stimulus such as tape strip, and applied fluorescence-labeled hapten and established the real-time imaging method in vivo.In addition, we have made it possible to evaluate the dynamics and cell migration of immune cells in the skin after that we transfer intravenously differentiated and cultured T cellsinto type Th1and Th2 T cells(Egawa et. Al. JID 2011 Apr).And more, we have shown that mDia1, which is one of the cytoskeletal molecules in the Rho family, play an essential role in the phagocytosis, migration, adhesion of skin dendritic cells, and immune synapse formation with the another immune cells, such as immunological synapse.Finally, we repoted that the contact dermatitis response is not formed at the time of the failure of this system(tanizaki et. al. Blood 2010 Dec)

决定皮肤免疫反应多样性的因素在很大程度上仍然是未知的。本研究的目的是阐明其在免疫应答中的作用和识别分子，特别是对外部刺激的免疫应答。我们使用Langerin-eGFP小鼠在稳态、相对较弱的刺激如胶带剥离、应用荧光标记的半抗原和建立体内实时成像方法中观察皮肤树突状细胞在一段时间内的动力学行为。此外，在我们将经静脉内分化和培养的T细胞转移到Th 1和Th 2型T细胞中之后，我们已经能够评估皮肤中免疫细胞的动力学和细胞迁移（Egawa et.此外，我们已经表明，作为Rho家族中的细胞骨架分子之一的mDia 1在皮肤树突状细胞的吞噬、迁移、粘附以及与另一种免疫细胞形成免疫突触（例如免疫突触）中发挥重要作用。我们报道在该系统失效时没有形成接触性皮炎反应（Tanizaki等，Blood 2010 Dec）

项目成果

Rho-mDia1 pathway is required for adhesion, migration, and T-cell stimulation in dendritic cells

• DOI: 10.1182/blood-2010-01-264150
• 发表时间: 2010-12-23
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Tanizaki, Hideaki;Egawa, Gyohei;Kabashima, Kenji
• 通讯作者: Kabashima, Kenji

Analysis of skin Langerhans cellsbehavior under the various conditionsusing in vivo live imaging

使用体内实时成像分析各种条件下皮肤朗格汉斯细胞的行为

• 发表时间: 2010

定常状態・抗原刺激時における皮膚Langerhans細胞のin vivo imaging

稳态和抗原刺激期间皮肤朗格汉斯细胞的体内成像

• 发表时间: 2010
• 作者: Hideaki Tanizaki;谷崎英昭
• 通讯作者: 谷崎英昭