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Joint Study on the protein-kinase-type photoreceptors

蛋白激酶型光感受器的联合研究

基本信息

批准号：
10044214
负责人：
WADA Masamitsu
金额：
$ 5.44万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10044214/
关键词：
photomorphogenesis photoreceptor chloroplast movement phototropism fern gametophyte 光屈性

项目摘要

1. Arabidopsis mutants which were deficient of chloroplast photoorientation movement were selected by a new method developed.2. Analytical studies on the causal gene of the mutants revealed that NPL1, a homologue of NPH1 which is a blue light receptor of phototropism in Arabidopsis, is a photo-receptor of chloroplast avoidance response induced by strong blue light.3. npl1 and nph1 double mutants lack chloroplast photoorietation movement as well as phototropism, meaning that both NPL1 and NPH1 are the photoreceptor of the two phenomona.4. NPH1 is a photoreceptor for chloroplast accumulation movement in the whole range of fluence rate tested. On the other hand, NPL1 is a receptor for accumulation under weak blue light but a receptor for avoidance under strong blue light.5. Adiantum PHY3 binds FMNs at the two LOV domains, suggesting that PHY3 is a possible photoreceptor for blue light as well as red/far-red light.6. PHY3 was found widely in advanced Pteridophytes but not in the lower ferns.7. By mutant anlyses and also by gene silencing method, it was found that PHy3 was a photoreceptor for red light-inducedchloroplast movement and phototropism in Adiantum.8. Adiantum NPL1 was sequenced and found that NPL1 was a photoreceptor of blue light-induced chloroplast avoidance response in Adiantum like in Arabidopsis.

1. 采用新方法对拟南芥叶绿体光取向运动缺陷突变体进行了筛选。突变体的致病基因分析表明，NPL1是拟南芥向光性蓝光受体NPH1的同源物，是强蓝光诱导叶绿体回避反应的光受体。npl1和nph1双突变体缺乏叶绿体的光取向运动和向光性，这意味着npl1和nph1都是这两种现象的光受体。NPH1在全通量范围内是叶绿体积累运动的光感受器。另一方面，NPL1在弱蓝光下是一个积累受体，而在强蓝光下是一个回避受体。Adiantum PHY3在两个LOV结构域结合FMNs，表明PHY3可能是蓝光和红光/远红光的光感受器。PHY3在晚期蕨类植物中广泛存在，而在下部蕨类植物中不存在。通过突变体分析和基因沉默的方法，发现PHy3是一种光感受器，用于红光诱导的叶绿体运动和向光性。对拟南芥（Adiantum） NPL1进行了测序，发现NPL1与拟南芥（Arabidopsis）一样是Adiantum叶绿体回避反应的光感受器。