Nucleotide sequence of a fern phytochrome cDNA and antibody production

通过与豌豆I型光色素cDAN克隆杂交鉴定的cDNA克隆（pFP1）的核苷酸序列，推导出了adiantum capillaveneres光色素的氨基酸序列。90%的核苷酸序列已完成，但仍有10%未确定。在n端区域，特别是在发色团附着位点附近，同源性较高，而在c端区域同源性较低。拟南芥和拟南芥光敏色素I、II、III型的氨基酸序列比较均未发现蕨类光敏色素与三种拟南芥中任何一种具有特殊的相似性。总体而言，蕨类植物光敏色素的亲水性与高等植物光敏色素非常相似，这表明尽管蕨类植物光敏色素已被证明是水溶性的，而不是跨膜蛋白。为了产生抗蕨类植物光敏色素的抗体，我们从pFP1的核苷酸序列中推断出由15个氨基酸组成的3个多肽。该克隆也产生了融合蛋白作为抗原。这种蕨类植物光敏色素单克隆抗体的生产正在进行中。

The amino acid sequence of adiantum capillus-veneres photochrome has been deduced form the nucleuotide suquence of a cDNA clone (pFP1) which was initially identified by hybridization to a pea type I poytochrome cDAN clone. Ninety percent of nucleotide sequence has been done but ten percent still remained unidentified. Homology is high at the N-terminal region, especially around the chromophore attachment site, but lower at C-terminal region. Comparison among the amino acid sequences of type I, II, III of Arabidopsis and Adiantum phytochrome did not show any special resembrance of fern phytochrome to any of the three types of Arabidopsis. Overall, the fern phytochrome is rather similar to higher plant phytochrome as far as hydropathy profile is concerned, suggesting that the phytochrome molecule is not trans-membranous protein but water soluble, although fern phytochrome has been shownTo raise anti-fern phytochrome antibodies, 3 polypeptides made of 15 amino acids around hydrophilic regions deduced from nucleuotide sequence of pFP1 were synthesized. Fusion protein of this clone was also produced as an antigen. Production of monoclonal antibodies of this fern phytochrome is now underway.