Nucleotide sequence of a fern phytochrome cDNA and antibody production

蕨类光敏色素 cDNA 的核苷酸序列和抗体生产

• 批准号: 02454014
• 负责人: WADA Masamitsu
• 金额: $ 4.35万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454014/
• 关键词: phytochorme; fern; anybody; cDNA; sequence; cloning

The amino acid sequence of adiantum capillus-veneres photochrome has been deduced form the nucleuotide suquence of a cDNA clone (pFP1) which was initially identified by hybridization to a pea type I poytochrome cDAN clone. Ninety percent of nucleotide sequence has been done but ten percent still remained unidentified. Homology is high at the N-terminal region, especially around the chromophore attachment site, but lower at C-terminal region. Comparison among the amino acid sequences of type I, II, III of Arabidopsis and Adiantum phytochrome did not show any special resembrance of fern phytochrome to any of the three types of Arabidopsis. Overall, the fern phytochrome is rather similar to higher plant phytochrome as far as hydropathy profile is concerned, suggesting that the phytochrome molecule is not trans-membranous protein but water soluble, although fern phytochrome has been shownTo raise anti-fern phytochrome antibodies, 3 polypeptides made of 15 amino acids around hydrophilic regions deduced from nucleuotide sequence of pFP1 were synthesized. Fusion protein of this clone was also produced as an antigen. Production of monoclonal antibodies of this fern phytochrome is now underway.

从与豌豆I型色素cDAN克隆杂交鉴定的cDNA克隆（pFP 1）的核苷酸序列推导出铁线蕨光色素的氨基酸序列。90%的核苷酸序列已经完成，但仍有10%尚未确定。同源性在N-末端区域高，尤其是在发色团连接位点周围，但在C-末端区域较低。比较拟南芥和铁线蕨光敏色素I、II、III型的氨基酸序列，没有发现蕨类光敏色素与拟南芥光敏色素中的任何一种光敏色素有任何特殊的区别。尽管蕨类光敏色素已被证明能产生抗蕨类光敏色素的抗体，但根据pFP 1的核苷酸序列，合成了3个由亲水区周围15个氨基酸组成的多肽。该克隆的融合蛋白也作为抗原产生。这种蕨类植物光敏色素的单克隆抗体的生产正在进行中。

项目成果

ITo,M.,A.Soejima & M.Wada: "Low genesic differentiation between Adicomtum capillusーveneris L.and A.ogasawarense Tagaws(Adientuceae),an endemic Pteaaopluy of the Bonisi (Ogasawara)Is'lands" Bot.Mag.Tokyo. 105. (1992)

ITo, M., A. Soejima & M. Wada：“Adicomtum capillus-veneris L. 和 A. ogasawaraense Tagaws（Adientuceae）之间的低遗传分化，这是 Bonisi（小笠原）群岛的特有 Pteaaopluy” Bot.Mag。 105.（1992）

Ito, M., A. Soejima, M. Wada: "Low genetic differentiation between Adiantum capillusveneris L. and A. ogasawarense Tagawa (Adiantaceae), an endemic pteridophyte of the Bonin (Ogasawara) Islands." Bot. Mag. Tokyo. 105. (1992)

Ito, M.、A. Soejima、M. Wada：“Adiantum capillusveneris L. 和 A. ogasawarense Takawa（Adiantaceae）（小笠原群岛的特有蕨类植物）之间的低遗传分化。”

Ito.M.,A.Soejima,M.Wada: "Low genetic differentiation between Adiantum capillus-veneris L.and A.ogasawarense Tagawa (Adiantaieae),an endemic pteridophyte of the Bonin (Ogasawara)Is lands." Bot.Mag.Tokyo. 105. (1992)

Ito.M.、A.Soejima、M.Wada：“铁线蕨 (Adiantum capillus-veneris L.) 和小笠原群岛 (Ogasawara) 群岛特有蕨类植物 A.ogasawarense Takawa (Adiantaieae) 之间的遗传分化较低。”