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Analytical study on the importance and the mechanism of chloroplast photorelocation movement

叶绿体光重定位运动的重要性及机制解析研究

基本信息

批准号：
13304061
负责人：
WADA Masamitsu
金额：
$ 33.45万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Importance of chloroplast avoidance response in plants.Using different classes of mutant, phot2 and chup1, defective in chloroplast avoidance movement, we show that these mutants are more susceptible to necrotic damage in high light than wild-type plants. We conclude that chloroplast avoidance movement actually reduces the amount of light absorption by chloroplasts, and might thus play an important role in the survival of plants under natural growth conditions.Function of phytochrome 3 in ferns.In some ferns, phototropism and chloroplast photorelocation are controlled by red light as well as blue light, but the photoreceptor had not been identified. We identify a function of phytochrome 3 (phy3), a chimera of phytochrome and phototropin, both for red light-induced phototropism and chloroplast photorelocation. Since phy3 greatly enhances the sensitivity to white light in orienting leaves and chloroplasts, this chimeric photoreceptor may have played a key role in the divergence and proli … More feration of fern species under low-light canopy conditions.CHUP1 function for organellar positioning and movement.CHUP1 encodes a protein containing multiple domains, such as an actin-binding domain and a proline-rich region. The fusion protein of CHUP1 and GFP expressed in leaf cells was targeted to the outer envelope of chloroplasts, suggesting CHUP1 localization in chloroplasts. CHUP1 was found to bind F-actin in vitro. CHUP1 is thus identified as a protein required for organellar positioning and movement in plant cells.Functional domain of PHOT2.The avoidance movement in Adiantum phot2 mutants could be restored by transient expression of non-mutant AcPHOT2 cDNA, indicating that the chloroplast avoidance movement in this fern is mediated by the Acphot2 protein. Further functional analyses of the Acphot2 protein were performed using this transient assay for chloroplast avoidance movement. The results obtained suggest that the LOV2, but not the LOV1, domain of Acphot2 is essential for avoidance movement. Less

叶绿体回避反应在植物中的重要性利用叶绿体回避运动缺陷的不同类型的突变体phot 2和chup 1，我们发现这些突变体比野生型植物更容易在强光下受到坏死损伤。我们的结论是，叶绿体的回避运动实际上减少了叶绿体的光吸收量，因此可能在自然生长条件下对植物的生存起着重要作用。光敏色素3在蕨类植物中的功能在某些蕨类植物中，向光性和叶绿体光移位受红光和蓝光控制，但光感受器尚未确定。我们确定了光敏色素3（phy 3）的功能，光敏色素和向光激素的嵌合体，红光诱导的向光性和叶绿体光迁移。由于phy 3在定向叶片和叶绿体时大大增强了对白色光的敏感性，这种嵌合光感受器可能在植物分化和增殖中起了关键作用。 ...更多信息 CHUP 1基因编码的蛋白质具有多个结构域，如肌动蛋白结合结构域和脯氨酸富集区。在叶片细胞中表达的CHUP 1和GFP融合蛋白定位于叶绿体的外被膜，表明CHUP 1定位于叶绿体。CHUP 1在体外可与F-肌动蛋白结合。PHOT 2的功能域。AcPHOT 2突变体的叶绿体回避运动可以通过非突变体AcPHOT 2 cDNA的瞬时表达而恢复，表明AcPHOT 2蛋白介导了该蕨类植物叶绿体的回避运动。Acphot 2蛋白质的进一步功能分析，使用这种瞬时测定叶绿体回避运动。所获得的结果表明，LOV 2，而不是LOV 1，域Acphot 2是必不可少的回避运动。少