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Searth fer adhesive molecule between plasma membrane and cell wall

Seath fer 质膜和细胞壁之间的粘附分子

基本信息

批准号：
07458196
负责人：
WADA Masamitsu
金额：
$ 4.74万
依托单位：
TOKYO METROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458196/
关键词：
fern protonema cell wall RGD peptide インテグリン細胞接着因子酢酸

项目摘要

The ralationship between cell wall and plasma membrane is thought to be very important in plants. Cell wall may play an important role to perform various physiological phenomena. In these phenomena, special proteins onnecting outer and inner parts of the cells through the plasma membrane are predicted. In this work we tried to find those proteins or their genes resembling to integrin (or integrine gene) as a receptor for extracellular matrix proteins.RGD peptide purchased from a company was found to be very effective for destruction of cortical microtubules, as well as for induction of morphological changes of nuclei and chloroplasts in fern Adiantum protonemal cells. However the peptide obtained from other company was not effective. The reason was caused by acetic acid mixed with RGD peptide which was used for prification process of the RGD peptide. The results were confirmed by experiments of the effect of acetic acid on the morphological changes of microtubules, nuclei and chloroplasts. So far we could not find any influence of RGD peptide on the developmental processes of fern protonema in the morphological point of view.Newt, we tried to search for integrin-related genes in Adiantum by RT-PCR,using degenerated primers constructed from the consensus sequences of mammalian integrin genes. Although we could obtain several amprified fragments, all flagments so far sequenced were not integrin-related proteins. At this moment, we could not prove the existance of integrin-related proteins or genes in Adiantum. Since there is not conclusive data of integrin or integrin-related proteins from plants, it is plausible that plants use other proteins for connecting inner and ourter parts of cells.

细胞壁与质膜之间的关系在植物中被认为是非常重要的。细胞壁在各种生理现象的发生中起着重要的作用。在这些现象中，通过质膜连接细胞内外的特殊蛋白质被预测。在这项工作中，我们试图找到与整合素（或整合基因）相似的蛋白或其基因作为细胞外基质蛋白的受体。从某公司购买的RGD肽对破坏蕨类植物原质细胞皮层微管、诱导细胞核和叶绿体形态变化非常有效。然而，从其他公司获得的肽并不有效。其原因是在RGD肽的纯化过程中，醋酸与RGD肽混合而成。通过乙酸对微管、细胞核和叶绿体形态变化的影响实验证实了这一结果。到目前为止，我们还没有从形态学角度发现RGD肽对蕨类植物原丝体发育过程的影响。在此基础上，我们利用基于哺乳动物整合素基因序列构建的退化引物，利用RT-PCR技术在Adiantum中寻找整合素相关基因。虽然我们可以获得一些扩增片段，但迄今为止测序的所有片段都不是整合素相关蛋白。目前，我们还不能证明整合素相关蛋白或基因在Adiantum中的存在。由于没有确凿的数据表明整合素或整合素相关蛋白来自植物，因此植物利用其他蛋白质连接细胞的内外部分是合理的。