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Searth fer adhesive molecule between plasma membrane and cell wall

Seath fer 质膜和细胞壁之间的粘附分子

基本信息

批准号：
07458196
负责人：
WADA Masamitsu
金额：
$ 4.74万
依托单位：
TOKYO METROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458196/
关键词：
fern protonema cell wall RGD peptide インテグリン細胞接着因子酢酸

项目摘要

The ralationship between cell wall and plasma membrane is thought to be very important in plants. Cell wall may play an important role to perform various physiological phenomena. In these phenomena, special proteins onnecting outer and inner parts of the cells through the plasma membrane are predicted. In this work we tried to find those proteins or their genes resembling to integrin (or integrine gene) as a receptor for extracellular matrix proteins.RGD peptide purchased from a company was found to be very effective for destruction of cortical microtubules, as well as for induction of morphological changes of nuclei and chloroplasts in fern Adiantum protonemal cells. However the peptide obtained from other company was not effective. The reason was caused by acetic acid mixed with RGD peptide which was used for prification process of the RGD peptide. The results were confirmed by experiments of the effect of acetic acid on the morphological changes of microtubules, nuclei and chloroplasts. So far we could not find any influence of RGD peptide on the developmental processes of fern protonema in the morphological point of view.Newt, we tried to search for integrin-related genes in Adiantum by RT-PCR,using degenerated primers constructed from the consensus sequences of mammalian integrin genes. Although we could obtain several amprified fragments, all flagments so far sequenced were not integrin-related proteins. At this moment, we could not prove the existance of integrin-related proteins or genes in Adiantum. Since there is not conclusive data of integrin or integrin-related proteins from plants, it is plausible that plants use other proteins for connecting inner and ourter parts of cells.

细胞壁和质膜之间的关系在植物中被认为是非常重要的。细胞壁在各种生理现象中起着重要作用。在这些现象中，预测了通过质膜连接细胞内外的特殊蛋白质。本工作试图寻找与整合素（或整合素基因）相似的蛋白质或基因作为细胞外基质蛋白的受体，发现从某公司购买的RGD肽对破坏铁线蕨原丝细胞皮层微管，以及诱导细胞核和叶绿体的形态变化非常有效。然而，从其他公司获得的肽是无效的。其原因是在制备RGD肽的过程中加入了乙酸。这一结果也被乙酸对微管、细胞核和叶绿体形态变化的影响实验所证实。Newt的研究表明，RGD肽对蕨类植物原丝体的发育过程没有影响，因此，我们利用哺乳动物整合素基因的保守序列构建了简并引物，通过RT-PCR的方法，在铁线蕨中寻找整合素相关基因。虽然我们可以获得几个扩增片段，但迄今为止测序的所有flagments都不是整合素相关蛋白。目前，我们还无法证明铁线蕨中存在整合素相关蛋白或基因。由于植物中整合素及其相关蛋白的研究还没有定论，植物利用其他蛋白连接细胞内外部的可能性很大。