STUDY ON THE MECHANISM OF DNAi IN FERN AND ITS APPLICATION

NEO 1基因的DNA片段（DNAi）在蕨类植物中通过胞嘧啶甲基化和希斯顿H3去乙酰化诱导基因沉默，并通过组蛋白去乙酰化抑制剂恢复，表明DNAi是由组蛋白去乙酰化诱导并转移到下一代的。为了证明DNAi在其他基因中的有效性，引入了在拟南芥叶绿体运动中不可缺少的KAC基因。叶绿体从质膜上分离并聚集。结果表明，DNAi在其他基因中也有效。

Gene silencing by DNA fragment of the NEO1 gene(DNAi) is induced in fern by cytosine methylation and histon H3 deacetylation and recovered by a histone deacetylation inhibitor, indicating that DNAi is induced by histone deacetylation and transferred to the next generation. To show the effectiveness of DNAi in other genes, KAC gene that is indispensable in chloroplast movement in Arabidopsis was introduced. Chloroplasts came apart from the plasma membrane and aggregated. The results showed the DNAi effectiveness in other gene.