STUDY ON THE MECHANISM OF DNAi IN FERN AND ITS APPLICATION

蕨类植物DNAi机制研究及其应用

• 批准号: 22657016
• 负责人: WADA Masamitsu
• 金额: $ 2.19万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657016/
• 关键词: シダ; 遺伝子機能; DNAi; サイレンシング; メチル化; ヒストン; 前葉体; 遺伝子発現

Gene silencing by DNA fragment of the NEO1 gene(DNAi) is induced in fern by cytosine methylation and histon H3 deacetylation and recovered by a histone deacetylation inhibitor, indicating that DNAi is induced by histone deacetylation and transferred to the next generation. To show the effectiveness of DNAi in other genes, KAC gene that is indispensable in chloroplast movement in Arabidopsis was introduced. Chloroplasts came apart from the plasma membrane and aggregated. The results showed the DNAi effectiveness in other gene.

NEO1 基因 DNA 片段（DNAi）在蕨类植物中通过胞嘧啶甲基化和组蛋白 H3 脱乙酰化诱导基因沉默，并通过组蛋白脱乙酰化抑制剂恢复，表明 DNAi 是由组蛋白脱乙酰化诱导并转移到下一代。为了显示DNAi对其他基因的有效性，引入了拟南芥叶绿体运动中不可缺少的KAC基因。叶绿体脱离质膜并聚集。结果显示DNAi对其他基因的有效性。