Functional Analysis of Newly-found Actin Structure Involved inChloroplast Photorelocation Movement

利用共焦激光扫描荧光显微镜和全内反射荧光显微镜对参与叶绿体运动的cp-肌动蛋白丝和介导cp-肌动蛋白丝聚合的chup1进行了详细分析。肌动蛋白丝的行为与细胞质肌动蛋白不同。它们被切割成0.3 μm左右的碎片并消失。肌球蛋白分子可能不参与叶绿体运动。参与叶绿体回避运动的Phot2可能定位在叶绿体外膜上。还获得了其他新的发现。

Cp－actin filaments that involved in chloroplast movement and CHUP1that mediates cp-actin filament polymerization were analyzed by a conforcal laserscanning fluorescence microscopy and total internal reflection fluorescence microscopyin detail. Cp-actin filaments` behavior was different from that of cytoplasmic actincables. They were severed and fragmented into about 0.3 μm and disappeared. Myosinmolecules might not be involved in chloroplast movement. Phot2 that involved inchloroplast avoidance movement might be localized on the chloroplast outer membrane.Other new findings were obtained.