Search for photoreceptor-binding proteins mediating photomorphogenesis and their localization
Search for photoreceptor-binding proteins mediating photomorphogenesis and their localization
批准号:
09440270
负责人:
WADA Masamitsu
金额:
$7.81万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998
中文摘要
筛选了具有向光性缺陷和叶绿体光取向运动缺陷的突变体。突变体分别由EMS和氮重离子束诱导。重离子束辐照获得的突变体中,有一个缺少拟南芥光敏色素发色基团结合域n端和nph1 c端几乎全长的嵌合基因AdiPHY3,表明这两种生理现象都是由PHY3介导的。研究了五种隐花苜蓿的表达模式。CRY1-3在配子体和孢子体的所有发育阶段均有表达。CRY4主要在孢子和暗生孢子体中表达。cry5主要存在于光生和暗生孢子体中。竞争性PCR结果显示,在红光诱导孢子萌发的条件下,CRY4表达下调,CRY5表达上调。隐花色素和GUS融合蛋白在洋葱表皮细胞中的表达模式表明,CRY3和cry4是在黑暗中转入细胞核的。采用双杂交方法筛选phy3结合蛋白候选基因。现在这些基因的特征正在进行中。
英文摘要
We screened mutants which are in defect of phototropism and chloroplast photoorientation movement in Adiantum protonemal cells. The mutants were induced by EMS or heavy ion beam of nitrogen. One of the mutants obtained by heavy ion beam irradiation lack AdiPHY3, which is a chimeric gene of phytochrome chromophore binding domain at N-terminus and almost full length of Arabidopsis NPH1at C-terminus, suggesting that the both physiological phenomena are mediated by PHY3.Expression pattern of five Adiantum cryptochromes were studied. CRY1-3 are expressed in all developmental stages tested both in gametophytes and in sporophytes. CRY4 is mainly expressed in spores and dark-grown sporophytes. CRY 5 is mainly in sporophytes grown both in the light and darkness. Competitive PCR shows that CRY4 is down- regulated and CRY5 is up-regulated when spores were irradiated with red light for the induction of spore germination. The expression pattern of fusion proteins of cryptochrome and GUS in onion epidermal cells suggests that CRY3 and 4 are transferred into nuclei in the dark.Candidates of genes of PHY3-binding proteins were screened by two-hybrid method. Now characterization of those genes are underway.
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Nozue, K., T.Kanegae, T.Imaizumi, S.Fukuda, H.Okamoto, K.-C.Yeh, J.C.Lagarias and M.Wada: "A phytochorme from the fern Adiantum with features of the putative photoreceptor NPH1." Proc.Natl.Acad.Sci.USA. 95. 15826-15830 (1998)
Nozue, K.、T.Kanegae、T.Imaizumi、S.Fukuda、H.Okamoto、K.-C.Yeh、J.C.Lagarias 和 M.Wada:“一种来自铁线蕨的植物色素,具有假定的光感受器 NPH1 的特征。
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K.Nozue: "Isdation of a second : phytochrome cDNA from Adintum capillus-veneris" Plant Physiology. 118. 711 (1998)
K.Nozue:“第二个的确定:来自 Adintum capillus-veneris 的光敏色素 cDNA”植物生理学。
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Kadota, A.and M.Wada: "Red light-aphototropic (rap) mutants lack red light-induced chloroplast relocation movement in the fern Adiantum capillus-veneris."Plant Cell Physiol.. 40. 238-247 (1999)
Kadota, A. 和 M.Wada:“红光反光性 (rap) 突变体在蕨类铁线蕨中缺乏红光诱导的叶绿体重新定位运动。”植物细胞生理学.. 40. 238-247 (1999)
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T.Kagawa: "Chloroplast-avoidance response induced by high-fluence blue light in prothallial cells of the fern Adiantum capilhes-veneris as analyzed by microbeam irradiation" Plant Physiology. 119. 917-923 (1999)
T.Kakawa:“通过微束照射分析铁线蕨原叶体细胞中高通量蓝光诱导的叶绿体回避反应”《植物生理学》。
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Adiantum capillus-veneris (Accession NO.AB016232). Kagawa, T.and M.Wada: "Chloroplast avoidance response induced by high fluence rate of blue light in prothallial cells of fern Adiantum as analyzed by microbeam irradiation." Plant Physiol.119. 917-923 (19
Adiantum capillus-veneris(登记号AB016232)。
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共 17 条
STUDY ON THE MECHANISM OF DNAi IN FERN AND ITS APPLICATION
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批准号:22657016
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.19万
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财政年份:2010
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负责人:WADA Masamitsu
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依托单位:
Functional Analysis of Newly-found Actin Structure Involved inChloroplast Photorelocation Movement
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批准号:20227001
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项目类别:Grant-in-Aid for Scientific Research (S)
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资助金额:$132.95万
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财政年份:2008
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负责人:WADA Masamitsu
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依托单位:
Analytical studies on signal transduction pathways of phot2 in chloroplast photorelocation movement
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批准号:17084006
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$66.24万
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财政年份:2005
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负责人:WADA Masamitsu
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依托单位:
Studies on the signal transduction and the mechanism of chloroplast photorelocation movement
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批准号:16107002
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项目类别:Grant-in-Aid for Scientific Research (S)
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资助金额:$66.73万
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财政年份:2004
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负责人:WADA Masamitsu
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依托单位:
Analytical study on the importance and the mechanism of chloroplast photorelocation movement
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批准号:13304061
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$33.45万
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财政年份:2001
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负责人:WADA Masamitsu
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依托单位:
Photoperception and its mechanism of blue light receptor phototropin in plants
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批准号:13139203
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$87.1万
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财政年份:2001
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负责人:WADA Masamitsu
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依托单位:
Joint Study on the protein-kinase-type photoreceptors
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批准号:10044214
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$5.44万
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财政年份:1998
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负责人:WADA Masamitsu
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依托单位:
Joint study on the search for phytochrome binding proteins
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批准号:09044232
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$4.48万
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财政年份:1997
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负责人:WADA Masamitsu
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依托单位:
Joint study on the search for phytochrome-binding protein
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批准号:07044206
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$9.54万
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财政年份:1995
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负责人:WADA Masamitsu
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依托单位:
Searth fer adhesive molecule between plasma membrane and cell wall
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批准号:07458196
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.74万
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财政年份:1995
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负责人:WADA Masamitsu
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依托单位:
Study on the induction of cell apex and its developent
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批准号:05454023
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.58万
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财政年份:1993
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负责人:WADA Masamitsu
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依托单位:
Nucleotide sequence of a fern phytochrome cDNA and antibody production
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批准号:02454014
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.35万
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财政年份:1990
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负责人:WADA Masamitsu
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依托单位:
国内基金
海外基金
CXCR4介导的小胶质细胞迁移在光感受器细胞变性中的作用及机制
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批准号:82371069
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项目类别:面上项目
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资助金额:49.00万元
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批准年份:2023
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负责人:李旌
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依托单位: