Joint study on the search for phytochrome-binding protein

寻找光敏色素结合蛋白的联合研究

基本信息

批准号：
07044206
负责人：
WADA Masamitsu
金额：
$ 9.54万
依托单位：
TOKYO METROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044206/
关键词：
Phytochrome protein kinase fern Lhcb moss protonema branching シダコケ藻類裸子植物

项目摘要

We have cloned 4 phytochrome genes and 3 cDNAs of them from fern Adiantum capillus-veneris. One of the sequences has a quite unique structure, i.e.N-terminus half of the gene has a conventional phytochrome structure, but C-terminus half has a structure of typical protein kinase. We also cloned and sequenced 5 genes and their cDNAs of blue light receptors. One of them has a predicted trans-membrane segment, indicating the protein encoded by this gene might localize in the plasma membrane and mediate chloroplast photoorientaion of apical swelling of protonemata. The photoreceptive site of these phenomena were found to be localized in the plasma membrane. We think that almost all photoreceptors involving the photomorphogenesis in fern are now in our hands. We are now characterizing which photoreceptor mediates which photoresponses, and we hope that we will dissolve signal transduction pathways of these photoreceptors in the near future.Red light induced gene expression was revealed in the first time in spore germination. Gene expression of light harvesting chlorophyll binding protein (Lhcb) was controlled by phytochrome, and red/farred photoreversibility was shown.Side branch formation in protonemata of the moss Ceratodon purpureus was found to be mediated by phytochrome. The frequency of red-light-induced branch formation differed between cells of differeny ages. The position of branching within the cell depended on the high absorption of red light. An aphototropic mutant ptr 116 which is deficient of biosynthesis of phytochrome chromophore, showed no branch formation by red light but was rescued by supplement of biliverdin, a precursor of phytochrome chromophore, in the culture medium.

我们已经克隆了4个植物色素基因，并从蕨类植物囊capillus-veneris克隆了3个cDNA。其中一个序列具有相当独特的结构，即基因的一半末端具有常规的植物色素结构，但是C-末端的一半具有典型的蛋白激酶的结构。我们还克隆并测序了5个基因及其蓝光受体的cDNA。其中一个具有预测的跨膜段，表明该基因编码的蛋白质可能定位于质膜中，并介导质子膜的顶层肿胀的叶绿体光剂量。发现这些现象的感光位点位于质膜中。我们认为，几乎所有涉及蕨类植物中光生生成的光感受器现在都掌握在我们手中。我们现在正在表征哪些光感受器介导了哪些光反应，我们希望我们能在不久的将来溶解这些光感受器的信号转导途径。红色光诱导的基因表达在孢子发芽中首次揭示。光收集叶绿素结合蛋白（LHCB）的基因表达由植物色素控制，并显示了红色/粘性的光疗法。发现Moss ceratodon purpureus的protonemata的侧面分支形成被发现由植物色素介导。在不同年龄的细胞之间，红光诱导的分支形成的频率有所不同。细胞内分支的位置取决于红光的高吸收。植物色素发色团的生物合成不足的过滤型突变体PTR 116没有通过红光形成分支，但在培养基中是通过补充植物色素成色的前体的补充来挽救的。