Study of the relation between the modulations of allergic response and the sick house syndrome caused by indoor air pollutants

室内空气污染物引起的过敏反应调节与病屋综合症关系的研究

• 批准号: 13470078
• 负责人: YOSHIDA Takahiko
• 金额: $ 9.09万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470078/
• 关键词: Sick house syndrome; sick building syndrome; formaldehyde; humoral immune response; immediate type allergy; enterobacteria; cytokine response in liver; 異数性染色体; 消化管細菌巣; 免疫毒性; アレルギー; Thバランス

It is well known that the immunological mechanism concerns in developing the sick building (house) syndrome (SBS,SHS), since allergy relating symptoms are frequently observed in the SBS patients. At the standing point of those findings, we exposed gaseous formaldehyde (HCHO) which is the major indoor air pollutant to cause SBS to mice (B6C3F1,female) and evaluated the health effects of HCHO by observing the modulations of the immune response and the experimentally induced allergic reactions. And we also surveyed the health effects which are reported in the SBS patients in the experimentally gaseous HCHO exposed mice without any immunological treatment. Furthermore we investigated the health effects of HCHO exposure via alimentary tract. Mice were exposed to HCHO by giving of HCHO treated animal diet pellets or HCHO added drinking water. For control group, intact diet and pure drinking water were given. Exposure of 500ppb gaseous HCHO enhanced the antibody forming response against sheep … More red blood cells and the lymphocyte proliferating response by mitogen stimuli. Enhancive modulations of immediate type allergic reaction were observed in both phases of sensitization and induction of symptoms. Aneuploid DNA in peripheral lymphocyte which is one of characteristic sign observed in some case of SBS patients was detected in gaseous HCHO exposed mice. Induction of prolactin mRNA in hippocampus and olfactory bulb was obvious in HCHO exposed mice. Ingestion of HCHO treated diet dramatically reduced the number of bacteria in alimentary tract dose dependency, whereas same quantity of HCHO via drinking water did weakly. Suppression of cytokine response in liver induced by i.p.of lipopolysaccharide, that phenomenon is observed in the mice with reduced number of alimentary tract bacteria, also observed in HCHO treated diet fed mice. The results indicate that both routes of HCHO exposure, inhalation or per oral, induce the modulation of immune response and allergic reactions as well as characteristic phenomena to SBS cases. It may suggest that immune function play some significant role in the development of SBS. Less

由于在SBS患者中经常观察到与过敏相关的症状，因此众所周知，免疫学机制与发生病态建筑（房屋）综合征（SBS，SHS）有关。在此基础上，本研究将引起SBS的主要室内空气污染物气态甲醛（HCHO）暴露于雌性小鼠（B6C3F1），并通过观察其对免疫反应的调节和实验诱导的过敏反应来评价HCHO对健康的影响。我们还调查了SBS患者在没有任何免疫治疗的实验气体HCHO暴露小鼠中所报告的健康影响。此外，我们还调查了经消化道暴露的HCHO对健康的影响。小鼠通过给予HCHO处理过的动物饲料颗粒或添加了HCHO的饮用水来暴露于HCHO。对照组饲喂完整日粮和纯净水。暴露于500ppb的HCHO气体中，增强了绵羊对有丝分裂原刺激的红细胞和淋巴细胞增殖反应。在致敏和诱导症状的两个阶段观察到立即型过敏反应的增强调节。在气体HCHO暴露小鼠外周血淋巴细胞中检测到非整倍体DNA，这是部分SBS患者的特征性表现之一。HCHO暴露小鼠海马和嗅球泌乳素mRNA的诱导作用明显。摄入HCHO处理过的饮食显著降低了消化道剂量依赖性细菌的数量，而通过饮用水摄入相同数量的HCHO则作用微弱。脂多糖对肝脏细胞因子反应的抑制作用，在消化道细菌数量减少的小鼠中观察到这种现象，在HCHO处理的日粮喂养小鼠中也观察到这种现象。结果表明，吸入和口服两种暴露途径均可诱导SBS患者的免疫反应和过敏反应及其特征现象的调节。提示免疫功能在SBS的发生发展中起重要作用。少