The production of transgenic pigs for xenotransplantation

用于异种移植的转基因猪的生产

• 批准号: 10556059
• 负责人: SATO Eimei
• 金额: $ 8.06万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10556059/
• 关键词: ES cells; nuclear transfer; embryo-derived cells; GFP; transgenic pigs; xenotransplantation; gene-knockout; somatic cell-clone; 遺伝子改革ブタ; 胚性幹細胞; 卵子; GFP発現細胞; 胚移植; ブタ; 異種移植; 遺伝子ノックアウトブタ; 発生工学

1) Basic technoloeies for the production of transgenic pigs, such as in vitro maturation and fertilization and culture of oocytes, nuclear transfer, freezing of embryos, embryo transfer, have been established.2) mitosen-activated protein kinase (MAPK) mediates the maturation-inducing signal from the cytoplasm into nucleus and induces meiosis reinitiation in porcine oocytes.3) The effect of hyaluronic acid on the development of in vitro-produced porcine embryos varies with the condition of oocyte maturation and fertilization.4) A porcine cell line can be established from the hatched blastocyst and maintained in vitro for a long period, and that reconstructed embryos obtained by transferring the blastocyst-derived cells into enucleated oocytes have the ability to develop to the blastocyst stage in vitro.5) Vector including a gene encoding green fluorescent protein (GFP gene) was developed, and techniques for selection of the cells with GFP gene was established .6) Techniques of nuclear transfer of the cells with GFP gene into enucleated oocytes was established.

1）为生产转基因猪的基本技术，例如体外成熟，肥料和卵母细胞的培养，核转移，胚胎的冻结，胚胎转移，胚胎转移，2）细胞激活的蛋白激酶（MAPK）介导了从细胞体和构成的成熟诱导信号，并指示了细胞的成熟信号。 oocytes.3) The effect of hyaluronic acid on the development of in vitro-produced porcine embryos varies with the condition of oocyte maturation and fertilization.4) A porcine cell line can be established from the hatched blastocyst and maintained in vitro for a long period, and that reconstructed embryos obtained by transferring the blastocyst-derived cells into enucleated oocytes have the ability为了在体外开发到胚泡阶段。5）开发了包括编码绿色荧光蛋白（GFP基因）基因的载体，并建立了用GFP基因选择细胞的技术.6）核转移的技术。建立了将GFP基因转移到具有GRFP基因中的细胞中的核转移技术。

项目成果

佐藤英明: "ティッシュエンジニアリング組織工学の基礎と応用"名古屋大学出版会. 289 (1999)

佐藤英明：《组织工程基础与组织工程应用》名古屋大学出版社289（1999）。

佐々田 比呂志: "インテグリンと生殖内分泌"ホルモンと臨床. 47(7). 639-646 (1999)

Hiroshi Sasada：“整合素和生殖内分泌”激素和临床科学 47(7) (1999)。

Eimei Sato: "Mannual for the Research of Mammalian Oocytes"Yokendo. 1-405 (2001)

佐藤英明：《哺乳动物卵母细胞研究手册》Yokendo。

佐藤英明: "移植臓器生産トランスジェニックブタの開発-作出戦略と研究の現状"研究ジャーナル. 23. 15-20 (2000)

佐藤秀明：“用于移植器官生产的转基因猪的开发 - 生产策略和研究的现状”研究杂志。23. 15-20 (2000)。

Eimei Sato: "Development of a gene-modified pig using a cloning technology"Chemistry and Biology. 38. 508-513 (2000)

佐藤英明：“利用克隆技术开发基因改造猪”化学与生物学。