Identification of genes and angogenic factors involving in selective growth and apoptosis of oocytes

1)明确了在IVM期间，猪COCs中表达的透明质酸结合蛋白，特别是CD44，可能在卵母细胞胞外空间形成基质网络，并在invm期间形成积云状扩张。2)促性腺激素的重复刺激对不孕率有良好的反应，T4处理增加了单个大鼠的排卵卵总数。3)猪卵母细胞促进了脑电图依赖性的积云细胞的扩增和has2 mRNA的表达，但这些对积云细胞的扩增和has2 mRNA的表达并不是必需的。在积云扩张过程中，HAS2参与HA的合成，eCG和pFF上调其表达。在卵母细胞中转录的有3 mRNA的表达谱与有2和CD44 mRNA的表达谱不同。CD44可能参与卵丘细胞与卵母细胞的相互作用。4)不同发育阶段卵泡血管篮的不同大小和结构支持了猪卵泡生长过程中卵泡血流量的逐渐增加。5)我们证明了在牛卵泡生长过程中血管生成增加，并且在卵泡内不同区域的血管生成不均匀。6)我们提供了一种创新的技术，通过基因和激素治疗来增强卵巢卵泡发育的诱导，从而预防卵巢功能障碍导致的不孕。7)建立了GDF-9基因片段卵巢直接注射技术。这可能为预防和治疗卵巢功能障碍性不孕症提供新的治疗方法。

1) It was clarified that Hyaluronic acid-binding proteins expressed in porcine COCs during IVM, particularly CD44, may form a network of the matrices in the extracellular space of the oocyte with cumulus expansion during INM.2) Infertile rdw rates responded well to repeated stimulation with gonadotropins, and T4 treatment increased the total number of ovulated eggs in an individual rat treated three times with gonadotropins.3) Porcine oocyte promote eCG-dependent cumulus expansion and the expression of has2 mRNA in cumulus cells, but these are not essential for expansion of cumulus cells and the expression of has2 mRNA. HAS2 is involved in HA synthesis during cumulus expansion, and eCG and pFF up-regulate its expression. The expresion profile of the has 3 mRNA that is transcribed in oocytes is different from those of has 2 and CD44 mRNA. CD44 may aprticipate in the interaction between cumulus cells and oocytes.4) Follicular vascular baskets of diverse sizes and architecture in various developmental stages support the gradua lincrease of follicular blood flow during follicule growth in the pig.5) We demonstrated that angiogenesis increases during bovine follicular growth and occurs uneven in different inner theca regions of the follicles.6) We offered an innovative technique for enhanced induction of follicular development in the ovary through gene and hormonal treatment, which may lead to prevention of infertility caused by ovarian dysfunction.7) A technique for direct ovarian injection of GDF-9 gene fragment was developed. It may contribute to novel therapy for prevention and treatment of infertility associated with ovarian dysfunction.