Identification of genes and angogenic factors involving in selective growth and apoptosis of oocytes

鉴定参与卵母细胞选择性生长和凋亡的基因和血管生成因子

• 批准号: 13460129
• 负责人: SATO Eimei
• 金额: $ 7.42万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460129/
• 关键词: Oogenesis; atresia; angiogenesis; HAS; CD44; thyroxine; VEGF; VEGF gene fragment; 卵子形成; TNF-α; bFGF; KLt-1; FLk; KDR; TNF-a; Klt-1; Flk; 卵胞発育; 血管網; FLK-1; FLT-1; 排卵; ブタ

1) It was clarified that Hyaluronic acid-binding proteins expressed in porcine COCs during IVM, particularly CD44, may form a network of the matrices in the extracellular space of the oocyte with cumulus expansion during INM.2) Infertile rdw rates responded well to repeated stimulation with gonadotropins, and T4 treatment increased the total number of ovulated eggs in an individual rat treated three times with gonadotropins.3) Porcine oocyte promote eCG-dependent cumulus expansion and the expression of has2 mRNA in cumulus cells, but these are not essential for expansion of cumulus cells and the expression of has2 mRNA. HAS2 is involved in HA synthesis during cumulus expansion, and eCG and pFF up-regulate its expression. The expresion profile of the has 3 mRNA that is transcribed in oocytes is different from those of has 2 and CD44 mRNA. CD44 may aprticipate in the interaction between cumulus cells and oocytes.4) Follicular vascular baskets of diverse sizes and architecture in various developmental stages support the gradua lincrease of follicular blood flow during follicule growth in the pig.5) We demonstrated that angiogenesis increases during bovine follicular growth and occurs uneven in different inner theca regions of the follicles.6) We offered an innovative technique for enhanced induction of follicular development in the ovary through gene and hormonal treatment, which may lead to prevention of infertility caused by ovarian dysfunction.7) A technique for direct ovarian injection of GDF-9 gene fragment was developed. It may contribute to novel therapy for prevention and treatment of infertility associated with ovarian dysfunction.

1)明确了在IVM期间在猪COCs中表达的透明质酸结合蛋白，特别是CD 44，可以在INM期间在卵丘扩展的卵母细胞的细胞外空间中形成基质网络。2）不育rdw率对促性腺激素的重复刺激反应良好，和T4处理增加了用促性腺激素处理三次的单个大鼠的排卵卵的总数。3）猪卵母细胞促进eCG-卵丘细胞的增殖和has 2 mRNA的表达依赖于卵丘细胞的增殖和has 2 mRNA的表达，但这不是卵丘细胞增殖和has 2 mRNA表达所必需的。HAS 2参与卵丘扩张过程中HA的合成，eCG和pFF上调其表达。在卵母细胞中转录的HAS 3 mRNA的表达谱与HAS 2和CD 44 mRNA的表达谱不同。CD 44可能参与卵丘细胞和卵母细胞之间的相互作用。4）不同发育阶段不同大小和结构的卵泡血管篮支持猪卵泡生长过程中卵泡血流量的逐渐增加。5）我们证明了牛卵泡生长过程中血管生成增加，并且在卵泡的不同内膜区域发生不均匀。我们提供了一种通过基因和激素治疗增强卵巢卵泡发育诱导的创新技术，这可能导致预防卵巢功能障碍引起的不孕症。7）开发了直接卵巢注射GDF-9基因片段的技术。这可能有助于新的治疗方法，以预防和治疗不孕症与卵巢功能障碍。

项目成果

Biolog of porcine cumulus-oocyte complexes ; Recent advances and possible significance on animal production

猪卵丘-卵母细胞复合物的生物学；

• 发表时间: 2003
• 期刊: Animal Frontier Sciences-Life science update in animal science
• 作者: 清水 隆;江 金益;飯島康二;宮林香奈子;小川好則;佐々田比呂志;佐藤英明;Eimei Sato
• 通讯作者: Eimei Sato

佐藤英明: "血管増殖促進因子を用いる新しい排卵誘発法"化学と生物. 41・4. 225-231 (2003)

佐藤英明：“利用血管生长促进因子的新排卵诱导方法”化学与生物学41・4（2003）。

佐藤英明: "Follicular microvasculature and angiogenic factors in the ovaries of domestic animals"J.Reprod.Dev.. 49・3. 181-192 (2003)

佐藤英明：“家畜卵巢中的卵泡微血管和血管生成因子”J.Reprod.Dev.. 49・3（2003）。

佐藤英明: "血管新生因子と卵子"Hormone Frontier in Gynecology. 11・1. 36-45 (2004)

佐藤秀明：“血管生成因子和鸡蛋”妇科激素前沿 11・1（2004 年）。

Animal Technology

动物技术

• 发表时间: 2004
• 作者: Takashi Shimizu;Jin-Yi Jiang;Koji Iijima;Kanako Miyabayashi;Yoshinori Ogawa;Hiroshi Sasada;Eimei Sato;佐藤英明;Eimei Sato
• 通讯作者: Eimei Sato