Improvement of IVM based on the elucidation of meiosis pause mechanism by uppermost stream factor identification of oocyte maturation inhibitory factor group

在前人研究成果的基础上，我们尝试对卵母细胞成熟过程中所见的减数分裂暂停机制的全球图像进行解释。根据DNA微阵列的数据搜索上游因子，结果表明C型利钠肽和肾上腺髓质素导致了上游因子的鉴定。此外，我们还研究了卵母细胞体外成熟度的改善，结果表明，在cAMP或cAMP增强因子的作用下，卵母细胞体外成熟一定时间后，受精量和受精后的发育能力都有所提高。

We tried the elucidation for the global image of the meiosis pause mechanism seen in oocyte maturation process based on the previous results of research. The upstream factor was searched based on the data of a DNA microarray, and it was shown clearly that C-Type natriuretic peptide and adrenomedullin lead to identification of an upstream factor. Furthermore, we also tackled improvement of IVM and it was shown clearly that fertilizing capacity and the developmental competence after fertilization go up by in vitro oocyte maturation for a definite period of time by cAMP or a cAMP potentiation factor.