Improvement of IVM based on the elucidation of meiosis pause mechanism by uppermost stream factor identification of oocyte maturation inhibitory factor group

我们尝试在前人研究成果的基础上，对卵母细胞成熟过程中减数分裂暂停机制的全局图像进行阐述。根据DNA芯片数据搜索上游因子，明确显示c型利钠肽和肾上腺髓素导致上游因子的鉴定。此外，我们还解决了IVM的改善问题，并清楚地表明，通过cAMP或cAMP增强因子在体外成熟一定时间的卵母细胞，受精能力和受精后的发育能力都有所提高。

We tried the elucidation for the global image of the meiosis pause mechanism seen in oocyte maturation process based on the previous results of research. The upstream factor was searched based on the data of a DNA microarray, and it was shown clearly that C-Type natriuretic peptide and adrenomedullin lead to identification of an upstream factor. Furthermore, we also tackled improvement of IVM and it was shown clearly that fertilizing capacity and the developmental competence after fertilization go up by in vitro oocyte maturation for a definite period of time by cAMP or a cAMP potentiation factor.