Porcine embryonic stem cells forming germ line chimeras

猪胚胎干细胞形成种系嵌合体

• 批准号: 07506005
• 负责人: SATO Eimei
• 金额: $ 9.92万
• 依托单位: Faculty of Agriculture, Tohoku University (1997)The University of Tokyo (1995-1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07506005/
• 关键词: germ line; germ lne chimera; primordial germ cell; chimera; LIF; embryonic stem cell; pig; xenotransplantation; 血血病抑制因子; 異種移植

1) Porcine 1-cell embryos were produced by the conventional or the new in vitro maturation/fertilization system and cultured in NCSU-23 supplemented with hyaluronic acid at various concentrations. Our results indicate that effect of hysluronic acid on development of in vitro produced porcine embryos varies with the conditions of maturation/fertilization of the oocytes.2) Porcine blastocysts were produced from immature oocytes by using _iVMFC,and cultured on STO feeder layrs to determine whether they can be used for studies on establishment of ES cells. when hatched blastocysts that had been obtained by culturing the intact blastocysts in the medium supplemented with FCS were transferred, they attached to the feeder cells. The results of the present study suggest that in vitro produced porcine blastocysts can be used for studies on establishment of ES cells.3) Our results demonstrated that single ES cells which maintain totipotency or pluripotency of high degree are present in a colony of ES cells, and that single ES cells cojoined with the blastomeres of 8-cell-stage embryos may colonize, if the circumustances allow, almost exclusively the germ cells and concomitantly the urogenital cell lineages.

1)用常规和新的体外成熟/受精系统获得猪1-细胞胚胎，并在NCSU-23中添加不同浓度的透明质酸进行培养。结果表明，不同的卵母细胞成熟/受精条件对猪胚胎体外发育的影响不同。2)用iVMFC从未成熟的卵母细胞中获得囊胚，并在STO饲养层上进行培养，以确定其是否可用于ES细胞的建立研究。在添加胎牛血清的培养液中培养完整的囊胚而获得的孵化的囊胚被移植到饲养层细胞上。本研究的结果表明，体外培养的猪囊胚可用于ES细胞的建立研究。3)我们的结果表明，在一群ES细胞中存在单个ES细胞，并且在条件允许的情况下，单个ES细胞与8-细胞期胚胎的卵裂球结合在一起，几乎可以只定植生殖细胞，并伴随着泌尿生殖细胞谱系。

项目成果

Guo, M.W., Mori, E., Sato, E., Saito, S., Mori, T.: "Expression of Fas ligand in murine oary." Am.J.Reprod.Immunol.37. 391-398 (1997)

郭，M.W.，森，E.，佐藤，E.，斋藤，S.，森，T.：“Fas配体在鼠卵巢中的表达。”

佐藤英明: "クローン羊" サイアス. 12. 72-73 (1997)

佐藤秀明：“克隆羊”赛亚斯。12. 72-73 (1997)

内藤邦彦: "Effects of vanadate on meiotic maturation of porcine oocytes in vitro" Journal of Reproduction and Development. 42(印刷中). (1996)

Kunihiko Naito：“钒酸盐对体外猪卵母细胞减数分裂的影响”，《生殖与发育杂志》42（出版中）。

佐藤 英明: "クローン羊" サイアイ. 12. 72-73 (1997)

佐藤秀明：“克隆羊”西爱 12. 72-73 (1997)

Inoue, M., Naito, K., Nakayama, T., Sato, E.: "Mitogen-activated protein kinase translocates into the germinal vesicle and induces germinal vesicle breakdown in porcine oocytes." Biol.Reprod.58. 130-136 (1998)

Inoue, M.、Naito, K.、Nakayama, T.、Sato, E.：“丝裂原激活蛋白激酶易位至生发囊泡并诱导猪卵母细胞生发囊泡破裂。”