Physiologically active substances regulating ovarian function

调节卵巢功能的生理活性物质

基本信息

批准号：
03454098
负责人：
SATO Eimei
金额：
$ 3.78万
依托单位：
The University of Tokyo (1992-1993)Kyoto University (1991)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

1) Polar body formation inhibitory protein : A protein, which inhibits polar body formation, was purified from a urine preparation from pregnant women. Amino terminal sequence analysis showed that residues 5 to 15 of this protein were identical to the residues 1 to 11 of human nonsecretory ribonuclease. Furthermore, residues 1 to 4 of this protein were identical to residues -4 to -1, corresponding to part of a single peptide region of eosinophil-derived neurotoxin, whose mature sequence is identical to nonsecretory ribonuclease.2) Glycosaminoglycans promoting viability of oocytes : OOcytes situated within the atretic follicles will undergo degeneration by segmentation, forming several to many "blastomeres" containing none to multiple nuclear fragments. A similar type of segmentation was observed in degenerating oocytes cultured in vitro. The observation that segmentation occurred more often with cumulus-free oocytes suggests that cumulus cells may produce a component(s) that would dela … More y the degenerative process. Glycosaminoglycans(GAGs) isolated from bovine follicular fluid(bFF) and added to the suspending medium prevented the occurrence of spontaneous segmentation in isolated cumulus-free mouse oocytes in a dose-dependent manner. To identify the active factor, specific GAG was purified from bFF.The fraction with a retention time nearly coincident with that of hyaluronic acid prevented the occurrence of segmentation.4) Ovarian angiogenesis and maturation of oocytes/follicles : In the ovary, there is a striking difference in the distribution of blood vessels to individual follicles. Epidermal growth factor (EGF), which induces capillary proliferation in vivo, was identified in granulosa, thecal and interstitial cells, suggesting that EGF is involved in neovascularization of the ovary. However, angiogenesis in the ovary is observed in specific regions of the follicles, especially the thecal layr. Thus it is surmized that ovarian components modulate the activity of ovarian EGF.We observed that GAGs isolated from mouse ovaries were potent enhancers of the activity of EGF by interacting or complexing with it. Less

1）极性体形成抑制蛋白：一种抑制极性体形成的蛋白质是从孕妇的尿液制备中纯化的。氨基末端序列分析表明，该蛋白的残留物5至15与人类非分泌核糖核酸酶1至11的残留物相同。此外，该蛋白的残留物1至4与残留-4至-1相同，与嗜酸性粒细胞衍生的神经毒素的单个肽区域的一部分相对应，其成熟序列的成熟序列与非秘密核糖酶相同。通过分割，形成了几至许多“胚泡”，其中含有多个核碎片。在体外培养的退化卵母细胞中观察到类似类型的分割。无云的卵母细胞更频繁地进行分割的观察结果表明，积云细胞可能会产生一个会产生的成分，这些成分会占据……更融合过程。从牛卵泡液（BFF）中分离并添加到悬浮培养基中的糖胺聚糖（GAGS）以剂量依赖性的方式阻止了在孤立的无库卢斯小鼠卵母细胞中的赞助分割。为了识别活性因子，从BFF中纯化了特异性的插科打术。保留时间几乎一致的部分与透明质酸的分数相吻合。4）4）卵巢血管生成和卵母细胞/卵泡的成熟：在卵巢中，血液容器分布在单个卵泡中存在引人注目的差异。在肉芽肿，thecal和间质细胞中鉴定出诱导体内毛细血管增殖的表皮生长因子（EGF），这表明EGF参与了卵巢的新生血管化。然而，在卵泡的特定区域，尤其是thecal layr中观察到卵巢中的血管生成。推测卵巢成分调节卵巢EGF的活性。我们观察到，从小鼠卵巢中分离出的插孔是通过与之相互作用或络合它的潜在EGF活性增强子。较少的