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Physiologically active substances regulating ovarian function

调节卵巢功能的生理活性物质

基本信息

批准号：
03454098
负责人：
SATO Eimei
金额：
$ 3.78万
依托单位：
The University of Tokyo (1992-1993)Kyoto University (1991)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

1) Polar body formation inhibitory protein : A protein, which inhibits polar body formation, was purified from a urine preparation from pregnant women. Amino terminal sequence analysis showed that residues 5 to 15 of this protein were identical to the residues 1 to 11 of human nonsecretory ribonuclease. Furthermore, residues 1 to 4 of this protein were identical to residues -4 to -1, corresponding to part of a single peptide region of eosinophil-derived neurotoxin, whose mature sequence is identical to nonsecretory ribonuclease.2) Glycosaminoglycans promoting viability of oocytes : OOcytes situated within the atretic follicles will undergo degeneration by segmentation, forming several to many "blastomeres" containing none to multiple nuclear fragments. A similar type of segmentation was observed in degenerating oocytes cultured in vitro. The observation that segmentation occurred more often with cumulus-free oocytes suggests that cumulus cells may produce a component(s) that would dela … More y the degenerative process. Glycosaminoglycans(GAGs) isolated from bovine follicular fluid(bFF) and added to the suspending medium prevented the occurrence of spontaneous segmentation in isolated cumulus-free mouse oocytes in a dose-dependent manner. To identify the active factor, specific GAG was purified from bFF.The fraction with a retention time nearly coincident with that of hyaluronic acid prevented the occurrence of segmentation.4) Ovarian angiogenesis and maturation of oocytes/follicles : In the ovary, there is a striking difference in the distribution of blood vessels to individual follicles. Epidermal growth factor (EGF), which induces capillary proliferation in vivo, was identified in granulosa, thecal and interstitial cells, suggesting that EGF is involved in neovascularization of the ovary. However, angiogenesis in the ovary is observed in specific regions of the follicles, especially the thecal layr. Thus it is surmized that ovarian components modulate the activity of ovarian EGF.We observed that GAGs isolated from mouse ovaries were potent enhancers of the activity of EGF by interacting or complexing with it. Less

1) 极体形成抑制蛋白：从孕妇的尿制剂中纯化出抑制极体形成的蛋白质。氨基末端序列分析显示该蛋白的残基5至15与人非分泌性核糖核酸酶的残基1至11相同。此外，该蛋白的残基 1 至 4 与残基 -4 至 -1 相同，对应于嗜酸性粒细胞衍生的神经毒素的单个肽区域的一部分，其成熟序列与非分泌性核糖核酸酶相同。2) 糖胺聚糖促进卵母细胞的活力：位于闭锁卵泡内的 OO 细胞将经历退化。分裂，形成几个到多个“卵裂球”，不包含任何到多个核片段。在体外培养的退化卵母细胞中观察到类似类型的分割。观察发现，无卵丘的卵母细胞更常发生分割，这表明卵丘细胞可能会产生一种或多种成分，从而延缓退化过程。从牛卵泡液（bFF）中分离出的糖胺聚糖（GAG）添加到悬浮介质中，以剂量依赖的方式阻止了分离的无卵丘小鼠卵母细胞自发分割的发生。为了鉴定活性因子，从bFF中纯化了特异性GAG。保留时间与透明质酸几乎一致的部分阻止了分割的发生。4)卵巢血管生成和卵母细胞/卵泡的成熟：在卵巢中，各个卵泡的血管分布存在显着差异。表皮生长因子（EGF）在体内诱导毛细血管增殖，在颗粒细胞、鞘细胞和间质细胞中被鉴定，表明EGF参与卵巢的新血管形成。然而，在卵泡的特定区域，尤其是卵泡层，观察到卵巢中的血管生成。因此推测卵巢成分调节卵巢EGF的活性。我们观察到从小鼠卵巢分离的GAG通过与EGF相互作用或复合而成为EGF活性的有效增强剂。较少的