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Regulatory mechanisms of selective growth and death of oocytes

卵母细胞选择性生长和死亡的调控机制

基本信息

批准号：
06454123
负责人：
SATO Eimei
金额：
$ 4.67万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

1) The active factor responsible for cumulus expansion was isolated from serum and follicular fluid.2) DbcAMP was involved in induction of fragmentation of porcine oocytes and HPLC-purified glycosaminoglycans prevented the activation of cAMP-dependent fragmentation.3) 17alpha-hydroxyprogesterone suppressed the angiogenic activity of hyaluronic acid-like HPLC-purified glycosaminoglycan prepared from extracts of non-luteal mouse ovaries.4) Immunoreactivity of TNF-alpha was found in the cytoplasm of the cells surrounding the maturing oocytes. Immunoblot analysis with the specific antibody to TNF-alpha identified the 17kD Mr band in the extract of cumulus-oocyte complexes. The process of degeneration of mouse oocytes was modulated by TNF-alpha accumulated in the expanded cumulus during oocyte maturation.5) Oocyte-cumulus cell complexes influenced the structure of the oviductal wall during the passage in the oviduct.6) When progesterone and its metabolites are present at high concentrations for a long period, they had the property of inducing in vitro parthenogenetic activation in mouse oocytes.7) Potential for the development of male half-embryos into fetuses was different from that of female half-embryos.8) The activation of MAP kinase is involved inthe regulation of meiotic maturation of porcine oocytes, and especially in the regulation after germinal vesicle breakdown.9) A clone corresponding to mRNA preferentially expressed in the functional staeg of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, which had been originally identified in male genital organs. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in porcine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs.

1)从血清和卵泡液中分离出促进卵丘扩张的活性因子。2)DbcAMP参与了猪卵母细胞碎裂的诱导，经高效液相色谱纯化的糖胺多聚糖阻止了cAMP依赖的碎裂的激活。3)17α-羟孕酮抑制了从非黄体小鼠卵巢提取液中制备的透明质酸样高效液相纯化糖胺聚糖的血管生成活性。4)在成熟卵母细胞周围的细胞质中发现了TNF-α的免疫反应。用抗肿瘤坏死因子-α抗体进行免疫印迹分析，证实卵丘-卵母细胞复合体提取液中有一条17kD的MR条带。在小鼠卵母细胞成熟过程中，卵母细胞-卵丘细胞复合体影响输卵管壁的结构。6)当黄体酮及其代谢产物长期高浓度存在时，它们具有诱导小鼠卵母细胞体外孤雌激活的特性。7)雄性半胚胎发育成胎儿的潜力不同于雌性半胚胎。8)MAP激活参与了猪卵母细胞减数分裂成熟的调节，9)发现了一个在黄体功能阶段优先表达的基因克隆，其编码的蛋白质类似于最初在男性生殖器官中发现的人类前列腺分泌蛋白。相反，尽管它们的产物在结构上相似，但在猪前列腺中没有检测到这个新发现的基因的表达，这表明这些结构相关的蛋白质在哺乳动物生殖器官中发挥着不同的作用。