Regulatory mechanisms of selective growth and death of oocytes

1)从血清和卵泡液中分离出导致积云扩张的活性因子。2) DbcAMP参与了猪卵母细胞断裂的诱导，高效液相色谱纯化的糖胺聚糖阻止了camp依赖性断裂的激活。3) 17 α -羟孕酮抑制非黄体小鼠卵巢提取物制备的透明质酸样糖胺聚糖的血管生成活性。4)成熟卵母细胞周围细胞的细胞质中存在tnf - α的免疫反应性。用tnf - α特异性抗体进行免疫印迹分析，在卵母细胞复合物提取物中鉴定出17kD Mr带。小鼠卵母细胞成熟过程中积聚在扩大的卵丘中的tnf - α调节了卵母细胞的变性过程。5)卵丘细胞复合物在输卵管内通过时影响了输卵管壁的结构。6)当孕酮及其代谢物长期高浓度存在时，具有诱导小鼠卵母细胞体外孤雌生殖激活的特性。7)雄性半胚胎发育成胎儿的潜力与雌性半胚胎不同。8) MAP激酶的激活参与了猪卵母细胞减数分裂成熟的调控，尤其参与了生发囊泡破裂后的调控。9)在黄体功能阶段优先表达的mRNA对应的克隆中发现了一种类似于人类前列腺分泌蛋白的蛋白，该蛋白最初在男性生殖器官中被发现。相比之下，尽管它们的产物结构相似，但在猪前列腺中未检测到该基因的表达，这表明这些结构相关蛋白在哺乳动物生殖器官中起着不同的作用。

1) The active factor responsible for cumulus expansion was isolated from serum and follicular fluid.2) DbcAMP was involved in induction of fragmentation of porcine oocytes and HPLC-purified glycosaminoglycans prevented the activation of cAMP-dependent fragmentation.3) 17alpha-hydroxyprogesterone suppressed the angiogenic activity of hyaluronic acid-like HPLC-purified glycosaminoglycan prepared from extracts of non-luteal mouse ovaries.4) Immunoreactivity of TNF-alpha was found in the cytoplasm of the cells surrounding the maturing oocytes. Immunoblot analysis with the specific antibody to TNF-alpha identified the 17kD Mr band in the extract of cumulus-oocyte complexes. The process of degeneration of mouse oocytes was modulated by TNF-alpha accumulated in the expanded cumulus during oocyte maturation.5) Oocyte-cumulus cell complexes influenced the structure of the oviductal wall during the passage in the oviduct.6) When progesterone and its metabolites are present at high concentrations for a long period, they had the property of inducing in vitro parthenogenetic activation in mouse oocytes.7) Potential for the development of male half-embryos into fetuses was different from that of female half-embryos.8) The activation of MAP kinase is involved inthe regulation of meiotic maturation of porcine oocytes, and especially in the regulation after germinal vesicle breakdown.9) A clone corresponding to mRNA preferentially expressed in the functional staeg of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, which had been originally identified in male genital organs. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in porcine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs.