Regulatory mechanisms of selective growth and death of oocytes

卵母细胞选择性生长和死亡的调控机制

基本信息

批准号：
06454123
负责人：
SATO Eimei
金额：
$ 4.67万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

1) The active factor responsible for cumulus expansion was isolated from serum and follicular fluid.2) DbcAMP was involved in induction of fragmentation of porcine oocytes and HPLC-purified glycosaminoglycans prevented the activation of cAMP-dependent fragmentation.3) 17alpha-hydroxyprogesterone suppressed the angiogenic activity of hyaluronic acid-like HPLC-purified glycosaminoglycan prepared from extracts of non-luteal mouse ovaries.4) Immunoreactivity of TNF-alpha was found in the cytoplasm of the cells surrounding the maturing oocytes. Immunoblot analysis with the specific antibody to TNF-alpha identified the 17kD Mr band in the extract of cumulus-oocyte complexes. The process of degeneration of mouse oocytes was modulated by TNF-alpha accumulated in the expanded cumulus during oocyte maturation.5) Oocyte-cumulus cell complexes influenced the structure of the oviductal wall during the passage in the oviduct.6) When progesterone and its metabolites are present at high concentrations for a long period, they had the property of inducing in vitro parthenogenetic activation in mouse oocytes.7) Potential for the development of male half-embryos into fetuses was different from that of female half-embryos.8) The activation of MAP kinase is involved inthe regulation of meiotic maturation of porcine oocytes, and especially in the regulation after germinal vesicle breakdown.9) A clone corresponding to mRNA preferentially expressed in the functional staeg of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, which had been originally identified in male genital organs. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in porcine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs.

1) The active factor responsible for cumulus expansion was isolated from serum and follicular fluid.2) DbcAMP was involved in induction of fragmentation of porcine oocytes and HPLC-purified glycosaminoglycans prevented the activation of cAMP-dependent fragmentation.3) 17alpha-hydroxyprogesterone suppressed the angiogenic activity of hyaluronic acid-like HPLC纯化的糖胺聚糖从非乳脂小鼠卵巢提取物中制备出来。4）TNF-α的免疫反应性在围绕成熟卵母细胞周围细胞的细胞质中发现。用特异性抗体的TNF-Alpha的免疫印迹分析鉴定了库藻素 - 元素复合物提取物中的17KD MR频带。小鼠卵母细胞变性的过程是由在卵母细胞成熟过程中累积的TNF-Alpha调节的。5）5）卵母细胞 - 肿瘤细胞复合物影响卵巢剂中的卵形壁的结构。在卵巢酮及其在高浓度上的典型范围内，它们在孕酮中的浓度很高，它们的质量很长时间。 7）7）雄性半胚胎进入胎儿的潜力与女性半胚胎的活化不同。8）MAP激酶的激活与减数分裂成熟的调节有关，或越或越的或其编码类似于人类前列腺分泌蛋白的蛋白质，该蛋白最初在男性生殖器官中鉴定出来。相反，尽管其产物的结构相似性，但在猪前列腺中未检测到该新鉴定的基因的表达，这表明这些与结构相关的蛋白质在哺乳动物生殖器官中起不同的作用。