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Identification of biological factors involving in serial culturing technique for germ cell series

生殖细胞系列连续培养技术涉及的生物因子的鉴定

基本信息

批准号：
10306018
负责人：
SATO Eimei
金额：
$ 22.78万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

1) Mitogen-activated protein kinase (MAPK) mediates the maturation-inducing signal from the cytoplasm into nucleus and induces meiosis reinitiation in porcine oocytes.2) Two isoforms of cDNA encoding novel zinc finger proteins which play important roles in the development of the testes was cloned.3) Both microtubules and microfilaments are involved in the developmental block in two-cell rat embryos and the developmental block in rat two-cell embryos is induced by disturrbance of mitochondrial energy metabolism. MAP kinase cascade is activated at the M-phase and some MAP kinases other than ERKs are activated during early cleavage of mouse embryos.4) Rapid detection of male-specific DNA sequence in bovine embryos using FISH method was established.5) Gonadotropin-inducible NAIP indirectly affect oocyte survival as a result of the inhibition of apoptotic granulosa-cell death during folliculogenesis.6) The effect of hyaluronic acid on the development of in vitro-produced porcine embryos varies with the condition of oocyte maturation and fertilization.7) A porcine cell line can be established from the hatched blastocyst and maintained in vitro for a long period, and that reconstructed embryos obtained by transferring the blastocyst-derived cells into enucleated oocytes have the ability to develop to the blastocyst stage in vitro.

1)丝裂原活化蛋白激酶（Mitogen-activated protein kinase，MAPK）介导成熟诱导信号从细胞质进入细胞核，并诱导猪卵母细胞减数分裂重新启动。2）克隆了两种编码新的锌指蛋白的cDNA亚型，它们在睾丸发育中起重要作用。3）微管和微丝都参与了大鼠二细胞胚胎的发育阻滞和大鼠二细胞胚胎的发育阻滞。细胞胚是由线粒体能量代谢紊乱引起的。4）建立了牛胚胎雄性特异性DNA序列的荧光原位杂交快速检测方法; 5）促性腺激素诱导的NAIP通过抑制卵泡发生过程中颗粒细胞的凋亡而间接影响卵母细胞的存活; 6）促性腺激素诱导的NAIP通过抑制卵泡发生过程中颗粒细胞的凋亡而间接影响卵母细胞的存活。透明质酸对体外生产的猪胚胎发育的影响随卵母细胞成熟和受精的条件而变化。7）从孵化的囊胚中可以建立猪细胞系并在体外维持较长时间，并且通过将胚泡来源的细胞转移到去核卵母细胞中获得的重构胚胎具有在体外发育到胚泡阶段的能力。