Identification of biological factors involving in serial culturing technique for germ cell series

生殖细胞系列连续培养技术涉及的生物因子的鉴定

• 批准号: 10306018
• 负责人: SATO Eimei
• 金额: $ 22.78万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10306018/
• 关键词: hyaluronic acid; MAP kinase; early embryogenesis; sex chromosome; oocyte maturation; in vitro fertilization; germ cells; embryo-derived cell; 卵子; 胚の凍結保存; サイクリンB; cdc25; 甲状腺ホルモン; 卵丘膨化; マウス; ブタ; 単為発生; 卵形成; 卵成熟; Primordial germ cells(PGC)

1) Mitogen-activated protein kinase (MAPK) mediates the maturation-inducing signal from the cytoplasm into nucleus and induces meiosis reinitiation in porcine oocytes.2) Two isoforms of cDNA encoding novel zinc finger proteins which play important roles in the development of the testes was cloned.3) Both microtubules and microfilaments are involved in the developmental block in two-cell rat embryos and the developmental block in rat two-cell embryos is induced by disturrbance of mitochondrial energy metabolism. MAP kinase cascade is activated at the M-phase and some MAP kinases other than ERKs are activated during early cleavage of mouse embryos.4) Rapid detection of male-specific DNA sequence in bovine embryos using FISH method was established.5) Gonadotropin-inducible NAIP indirectly affect oocyte survival as a result of the inhibition of apoptotic granulosa-cell death during folliculogenesis.6) The effect of hyaluronic acid on the development of in vitro-produced porcine embryos varies with the condition of oocyte maturation and fertilization.7) A porcine cell line can be established from the hatched blastocyst and maintained in vitro for a long period, and that reconstructed embryos obtained by transferring the blastocyst-derived cells into enucleated oocytes have the ability to develop to the blastocyst stage in vitro.

1）有丝分裂原激活的蛋白激酶（MAPK）将细胞质的成熟信号介导到核中，并诱导猪卵母细胞中的减数分裂重新生物。2）cDNA的两个同种型）的两个编码新颖的锌指蛋白在锌指蛋白中扮演了重要的锌蛋白。两细胞大鼠胚胎的发育阻滞和大鼠两细胞胚胎中的发育阻滞是由线粒体能量代谢的蒸馏而引起的。 MAP kinase cascade is activated at the M-phase and some MAP kinases other than ERKs are activated during early cleavage of mouse embryos.4) Rapid detection of male-specific DNA sequence in bovine embryos using FISH method was established.5) Gonadotropin-inducible NAIP indirectly affect oocyte survival as a result of the inhibition of apoptotic granulosa-cell death during 6）透明质酸对体外生产的猪胚胎发展的影响随卵母细胞成熟和受精的条件而变化。7）7）猪细胞系可以从孵化的胚泡中确定，并在长时间内通过在体外维持的胚胎中的胚胎造成的胚胎构造的卵形卵形的卵形良好的卵形构造的卵形分析，并维持在体外，并维持效率。在体外发展到胚泡阶段的能力。

项目成果

Eimei Sato: "Reproductive Biology in Mammals"Gakusousha. 1-300 (1999)

佐藤英明：“哺乳动物的生殖生物学”Gakusousha。

佐藤英明: "Asparagine-linked glycosylation of the rat leukemia inhibitory factor expressed by Simian COS7 cells" Bioscience,Biotechnology and Biochemistry. 62. 1318-1325 (1998)

Hideaki Sato：“猿 COS7 细胞表达的大鼠白血病抑制因子的天冬酰胺连接糖基化”《生物科学、生物技术和生物化学》62. 1318-1325 (1998)。

佐藤英明: "Evaluation of fluid from cystic follicles for in vitro maturation and fertilization of bovine oocytes" Theriogenology. 50. 307-320 (1998)

Hideaki Sato：“牛卵母细胞体外成熟和受精的囊泡液体评估”Theriogenology。50. 307-320 (1998)。

佐藤英明: "Newtreuds in Micro anatomy of Reproduction"Mozzon Giuntina, Firenze. 356 (1999)

佐藤秀明：“生殖微观解剖学中的新事物”Mozzon Giuntina，佛罗伦萨 356 (1999)。

佐藤英明: "Effect of hyaluronic acid on the development of porcine 1-cell embryos produced by a new NMF system"Theriogenology. 51. 777-784 (1999)

Hideaki Sato：“透明质酸对新 NMF 系统产生的猪 1 细胞胚胎发育的影响”Theriogenology 51. 777-784 (1999)。