Bone Regeneration with the use of Tissue Engineering

利用组织工程进行骨再生

• 批准号: 11558104
• 负责人: TAKAKUDA Kazuo
• 金额: $ 6.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558104/
• 关键词: Biomaterials; Bone; Reconstructive Surgery; Tissue Regeneration; Animal Experiments; 組織工学; 再生医工学; 組織誘導再生

We investigated new bioabsorbable inorganic/organic composite materials, that are b-TCP/PLGC for the GBR membrane of which matrix is biodegradable polymer (PLGC) and fillers are beta-tricalcium phosphate (b-TCP). Experimental animals were male Beagle and Mongrel dogs of ca 1 year old, and male Japanese white rabbits of body weight ca 2.5kg and male SD rats of 8 to 10 weeks old. For the b-TCP/PLGC membranes, various experimental of GBR treatments were examined. The one was for mandibular bones. The bone defects of 1Ox10x1Omm were made and covered with the bioabsorbable membranes. The bone formations within the bone defects were examined with X-rays in every 4 weeks, and it was shown that the defects were filled with bone tissues after 12 weeks. The experimental bone defects of 20mm length in canine tibia were similarly treated. The defects were filled with bone tissues except central cartilage tissues of several mm lengths. The canine skulls with holes of 10mm diameter were similarly treated and bone formations were observed. No bone formations were observed in control specimens in every case. The transplantation of periosteal membrane were tried with the use of the bioabsorbable membrane and found that they enhance the early bone formation significantly.

研究了新型生物可吸收无机/有机复合材料，即以生物可降解聚合物(PLGC)为基质，以β-磷酸三钙(b-TCP)为填充物的生物可吸收无机/有机复合材料。实验动物为1岁左右的雄性Beagle和Mongrel犬，体重2.5 kg左右的雄性日本大白兔和8~10周龄的雄性SD大鼠。对于b-TCP/PLGC膜，考察了GBR处理的各种实验。一个是关于下颌骨的。造成10x10×100 mm的骨缺损处，覆盖生物可吸收膜。每隔4周行骨缺损区X线片检查，12周后缺损区已被骨组织充填。对犬胫骨20 mm长的实验性骨缺损进行同样的处理。除几毫米长的中央软骨组织外，其余缺损区均由骨组织填充。对直径为10 mm的犬头骨进行类似处理，观察其成骨情况。所有病例的对照标本均未见骨形成。将生物可吸收膜应用于骨膜移植，发现其能明显促进早期骨形成。

项目成果

小山富久,高久田和夫,菊池正紀,田中順三,松本裕子,宮入裕夫: "β-TCP/ポリ乳酸系コポリマー膜による骨再建(第3報 遊離骨膜による骨再建)"バイオメカニクス学会雑誌. 6.1. 31-32 (2000)

Tomihisa Koyama、Kazuo Takakuda、Masaki Kikuchi、Junzo Tanaka、Yuko Matsumoto、Hiroo Miyairi：“使用 β-TCP/聚乳酸共聚物膜进行骨重建（第三次报告：使用游离骨膜进行骨重建）”日本生物力学学会杂志 6.1。 .31-32 (2000)

Kikuchi M,Tanaka J,Koyama Y,Takakuda K: "Cell Culture Test of TCP/CPLA Composite"Journal of Biomedical Materials. 48. 108-110 (1999)

Kikuchi M，Tanaka J，Koyama Y，Takakuda K：“TCP/CPLA复合材料的细胞培养测试”生物医学材料杂志。

小山富久,高久田和夫,菊池正紀,田中順三,松本裕子,宮入裕夫,青木美香,永岡勝好,小坂俊文,田中茂男: "β-TCP/ポリ乳酸系コポリマー膜による骨再建(第3報遊離骨膜による骨再建)"顎顔面バイオメカニクス学会雑誌. 6. 31-32 (2000)

Tomihisa Koyama、Kazuo Takakuda、Masaki Kikuchi、Junzo Tanaka、Yuko Matsumoto、Hiroo Miyairi、Mika Aoki、Katsuyoshi Nagaoka、Toshifumi Kosaka、Shigeo Tanaka：“使用 β-TCP/聚乳酸共聚物膜进行骨重建（第 3 次报告）”使用 β-TCP/聚乳酸共聚物膜进行骨重建游离骨膜）”颌面生物力学学会杂志。6. 31-32 (2000)

小山富久: "生体吸収性有機/無機複合体膜を利用した下顎骨再建に関する基礎的研究"口腔病学会誌. 67-1. 63-69 (2000)

Tomihisa Koyama：“使用生物可吸收有机/无机复合膜进行下颌重建的基础研究”口腔医学会杂志 67-1（2000）。

T Koyama Y, K Takakuda, H Miyairi, M Kikuchi, J Tanaka: "Guided Bone Regeneration with Bioabsorbable Organic/Inorganic Composite Membrane"Proceedings of the International Workshop on Bio-integraged Materials and Tissue Engineering. 70 (2000)

T Koyama Y、K Takakuda、H Miyairi、M Kikuchi、J Tanaka：“利用生物可吸收有机/无机复合膜引导骨再生”生物集成材料和组织工程国际研讨会论文集。