Development of bioabsorbable artificial dura mater

生物可吸收人工硬脑膜的研制

• 批准号: 14380394
• 负责人: TAKAKUDA Kazuo
• 金额: $ 4.99万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380394/
• 关键词: Biomaterials; Dura matter; Regeneration; Cerebrospinal fluid; Leakage

Bloabsorbable artificial dura matter was investigated. Experimental membranes made from copolymers of L-Iactlc acid, glycolic acid and caprolactone were developed. The effectiveness of the materials was firstly verified with a leaking test In vitro. The developed membranes were sutured with swine dura matter and amount of leaked saline under determined pressure upto 6OmmHg, the maximum brain pressure in clinical situation, were measured. Although the leak from a needle hole was prevented, the leak through the stitch under high pressure was observed. This type of leakages was found to be dependent on the method of suturing, worst in the cases of single knot with high suture tension, whereas the continuous simple suturing with less tension and suitable pitch gave the best results and almost complete prevention of leakage was possible. Next we examined the biocompatibilities of the materials with the In vivo test In which we used rats as experimental animals. Artificial defects of 5x10mm were made in each side of rats dura matter. The developed membranes were set on the defects and sutured. The specimens were retrieved after 4, 8, and 12 weeks of implantation. Leakage, regeneration, and surrounding tissues were examined. 4 weeks postoperation, the developed membranes were surrounded with the soft fibrous tissues. The leakage tests were carried out and no leakage was proved under 60mmHg of pressures. Histological observation verified no inflammation response after 12 weeks, and revealed the reasonable regeneration of dura matter.

研究了可吸收血液的人工硬脑膜物质。开发了由 L-乳酸、乙醇酸和己内酯的共聚物制成的实验膜。该材料的有效性首先通过体外泄漏测试得到验证。将形成的膜与猪硬脑膜缝合，并在高达60mmHg（临床情况下的最大脑压）的确定压力下测量泄漏的盐水量。尽管防止了针孔的泄漏，但在高压下观察到通过针迹的泄漏。发现这种类型的泄漏取决于缝合方法，在高缝合张力的单结情况下最糟糕，而具有较小张力和合适节距的连续简单缝合给出了最好的结果，并且几乎可以完全防止泄漏。接下来，我们通过体内测试检查了材料的生物相容性，其中我们使用大鼠作为实验动物。在大鼠硬脑膜两侧制作5×10mm的人工缺损。将显影后的膜放置在缺损处并缝合。植入4周、8周和12周后取出样本。检查渗漏、再生和周围组织。术后4周，发育好的膜被软纤维组织包围。进行泄漏测试，证明在60mmHg压力下不泄漏。组织学观察证实12周后没有炎症反应，并揭示了硬脑膜的合理再生。

项目成果

高久田和夫, 富永勉, 大野喜久郎, 向井智和, 白浜憲昭: "生体吸収性人工硬膜の開発"日本機械学会第16回バイオエンジニアリング講演会講演論文集. 03-38. 293-294 (2004)

Kazuo Takakuda、Tsutomu Tominaga、Kikuo Ohno、Tomokazu Mukai、Noriaki Shirahama：“生物可吸收人工硬脑膜的开发”第 16 届日本机械工程师学会生物工程会议记录 03-38（2004 年）。

Kazuo Takakuda: "Development of Bioabsorbable Artificial Dura matter"Proceeding of 16^<th> Conference on Bioengiinerring. 293. (2004)

Kazuo Takakuda：“生物可吸收人工硬脑膜物质的开发”第 16 届生物工程会议论文集。

高久田和夫 ほか: "生体吸収性人工硬膜の開発"日本機械学会バイオsンジニアリング講演会抄録集. 03-38. 293-294 (2004)

Kazuo Takakuda 等人：“生物可吸收人工硬脑膜的开发”日本机械工程师学会生物工程会议摘要 03-38 (2004)。