Basic studies on remodeling of fibrous tissues and their application to reconstruction of ligaments

纤维组织重塑的基础研究及其在韧带重建中的应用

• 批准号: 17300145
• 负责人: TAKAKUDA Kazuo
• 金额: $ 8.27万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300145/
• 关键词: Biomechanics; Biomaterials; Mechanical Stimuli; Tissue Engineering; Ligaments; Remodeling; バイオメカニックス; 人工臓器工学; 生体物性; 生体制御

1. Design of devices for ligament reconstruction considering the tissue remodeling Polyester fabrics composed of sparsely aligned fibers were utilized as the model grafts for the artificial ligaments. the biological fixation was expected to be realized through the spontaneous bone ingrowth into the space between fibers. experimental anterior cruciate ligament reconstruction was performed with using the rabbit model. the mechanical test and histometric measurement demonstrated that the attachment strength and amount of bone area were significantly large in the group of chitin-coated grafts. thus biological fixation was successfully realized and the possibility of ACL reconstruction was demonstrated.2. Biological fixation of percutaneous devices and dental implants with use of the guided remodeling of collagen fibers. Model devices were developed to which spontaneous anchoring of surrounding soft fibrous tissue was realized. implantation test with using rat's hind skin demonstrated the a … More ll specimens without anchoring structure dropped out of skin, whereas the specimens equipped with the anchoring structure did not Further, histological observation revealed the spontaneous anchoring of collagen fibers. such spontaneous anchoring is expected to be beneficial for percutaneous devices and dental implants.3. In vitro remodeling of soft fibrous tissues under mechanical stimuli Femoral fascia of rat, a thin fibrous soft tissue surrounding muscle, in which orientation of collagen fibers can be monitored with a light microscope was cultured under the mechanical stimuli of static stretching. In case of the culture free from load, the orientation of fibers became random, and the toe region in the load-displacement curve disappeared. Contrarily, the specimens subjected to incremental small stretching demonstrated highly aligned collagen fibers and extended toe region. On the other hand, the tissue subjected to a large stretch demonstrated aligned collagen fibers and small toe region. Thus suitable magnitude of mechanical stimuli induced the realignment of collagen fibers and adoptive change of mechanical properties, whereas large stimuli do not trigger remodeling. Less

1.考虑组织重塑的韧带重建装置的设计采用由稀疏排列的纤维组成的聚酯织物作为人工韧带的模型移植物。生物固定有望通过骨自发长入纤维之间的空间来实现。使用兔模型进行实验性前交叉韧带重建。机械测试和组织测量表明，甲壳素涂层移植物组的附着强度和骨面积显着较大。从而成功实现了生物固定，证明了ACL重建的可能性。 2．利用胶原纤维的引导重塑对经皮装置和牙种植体进行生物固定。开发了模型装置，实现了周围软纤维组织的自发锚定。使用大鼠后皮肤的植入试验表明，没有锚定结构的标本从皮肤中脱落，而配备锚定结构的标本则没有。此外，组织学观察揭示了胶原纤维的自发锚定。这种自发锚定预计将有利于经皮装置和牙种植体。3．机械刺激下软纤维组织的体外重塑在静态拉伸的机械刺激下培养大鼠股骨筋膜，这是一种围绕肌肉的薄纤维软组织，其中胶原纤维的方向可以用光学显微镜监测。在无负载培养的情况下，纤维的方向变得随机，并且负载-位移曲线中的脚趾区域消失。相反，经过增量小拉伸的样本表现出高度排列的胶原纤维和延伸的脚趾区域。另一方面，受到大拉伸的组织表现出对齐的胶原纤维和小脚趾区域。因此，适当强度的机械刺激会诱导胶原纤维的重新排列和机械性能的过继变化，而大的刺激不会触发重塑。较少的

项目成果

Mechanical attachment of soft fibrous tissues to implants by using mesh structures.

使用网状结构将软纤维组织机械附着到植入物上。

• 期刊: Clinical Oral Implants Research (印刷中)
• 作者: S. Asoda;T. Arita;H. Koshitomae;and K. Takakuda
• 通讯作者: and K. Takakuda

Mechanical Attachment of Soft Fibrous Tissues to Artificial Materials with Mesh Structures

软纤维组织与网状结构人造材料的机械附着

• 发表时间: 2007
• 作者: Takakuda K;Asoda S;Arita T.
• 通讯作者: Arita T.

組織の「つなぎめ構造」再建のバイオメカニクス

组织“系链结构”重建的生物力学

• 发表时间: 2007
• 期刊: メディカルバイオ 4
• 作者: T. Yamada;Y. Koyama;T. Kawai;T. Muneta;and K. Takakuda;高久田和夫
• 通讯作者: 高久田和夫

キチン-ポリエステル不織布を用いた靭帯再建

使用甲壳素聚酯非织造布重建韧带

• 发表时间: 2006
• 作者: 川合智行;蓮見祐之;永井正洋;小山富久;高久田和夫.
• 通讯作者: 高久田和夫.

Remodeling of Soft Fibrous tissues under mechanical Stimuli-an in vitro experiment

机械刺激下软纤维组织的重塑-体外实验

• 发表时间: 2005
• 作者: Tamura Y;Matsumoto H;Takakuda K.
• 通讯作者: Takakuda K.