Capillary Sequencer

高分辨率毛细管电泳法是精确鉴定DNA多态的标准方法。毛细管测序仪的主要应用是高通量地对不同动植物物种中的位点特异性DNA标记进行高通量基因分型，如高度可变的核微卫星标记。该仪器还将用于正在进行的和拟议的项目中，对各种不同生物群体中的特定DNA条形码区域进行测序。高精度的基因分型对于种群遗传和法医应用是必不可少的，例如基因流和亲子关系分析，以及对遗传变异的评估。这种方法今后还将被用作在保护方案框架内监测遗传资源和人类对生物多样性的影响的标准方法。对代表性遗传标记的更大样本量的分析对于在著名科学期刊上发表变得更加重要。新仪器将允许在多重反应中分析更大的样本量(每周数千个样本)，正如正在进行的和拟议的项目中所预期的那样。由于基因分型方法(例如微卫星、SNPs、全基因组AFLP)和DNA条形码区域的测序范围广泛，该仪器将被不同系(林业、生物学)的不同研究小组使用，以确保该系统的持续使用。该仪器将成为一个服务单位的一部分，该服务单位还包括一个质谱仪，用于大规模评估单核苷酸多态和表观遗传变异。

High-resolution capillary electrophoresis is a standard method for precise identification of DNA polymorphisms. The main application of the capillary sequencer is the high-throughput genotyping of locus-specific DNA markers such as highly variable nuclear microsatellite markers in different plant and animal species. The instrument will also be used in ongoing and proposed projects for the sequencing of specific DNA-barcoding regions in a variety of different organismal groups. High-precision genotyping is indispensable for population genetic and forensic application such as gene flow and parentage analyses and for the assessment of genetic variation. This method will be used also in the future as standard method for the monitoring of genetic resources and of human impacts on biological diversity in the framework of conservation programs. The analysis of larger sample sizes at representative genetic markers is becoming even more important for the publication in renowned scientific journals. The new instrument would allow the analysis of larger sample sizes in multiplex reactions (several thousand samples per week) as anticipated in ongoing and proposed projects. Because of the wide spectrum of genotyping methods (e.g., microsatellites, SNPs, genome-wide AFLPs) and the sequencing of DNA-barcoding regions, the instrument would be used by different research groups of different faculties (Forestry, Biology), securing a continuous utilization of the system. The instrument would become part of a service unit which also includes a Mass spectrometer for the large-scale assessment of Single Nucleotide Polymorphisms (SNPs) and of epigenetic variation.