MOLECULAR ANALYSIS OF ORIGIN OF JAPANESE DOMESTIC DOGS BY ANCIENT DNA

利用古代DNA对日本家犬的起源进行分子分析

• 批准号: 12460121
• 负责人: ISHIGURO Naotaka
• 金额: $ 3.84万
• 依托单位: OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460121/
• 关键词: DNA; dog; mitochondria DNA; archaeology; ancient; haplotype; phylogeny; jomon

Sequences of ancient mitochondrial DNA (mtDNA) from archaeological remains of Japanese dogs were determined from 145 ancient dog samples (mainly bones) from the Jomon, Yayoi, Kofun, Okhotsk and the kamakura Periods. Highly variable sequences of the mitochondrial control region (198bp) were amplified at least twice from independently prepared DNA extracts, and those from ancient samples were compared with * haplotypes from 178 modern dogs. The 198bp ancient mtDNA was amplified from 74 ancient dog samples, and the sequences were classified into 19 haplotypes comprising five modern haplotypes (M1, M2, M5, M10 and M11) identified in modern dogs. Three major clusters (CL1 to CL3) were distinguished within the modern dog control region using phylogenetic analysis; all ancient dogs belonged to the CL1 cluster. Origin of Hokkaido dogs was considered to be from southern dog population, but not from northern dog population. Ancient dogs in Okinawa Islands was derived from Eurasian continent through trade activity, and dogs in Gusuku Period had the same haplotypes to modern Ryukyu dog populations. The mtDNA from Japanese wolf was also determined, the genetical relationship among Japanese wolf, dogs and wolves were investigated. The phylogenetic analysis of mtDNA from Japanese wolf with those from dogs and wolves indicated that Japanese wolf genetically related to dogs and wolves from Eurasian continent.To reinforce the canine mtDNA database, 600bp of mtDNA D-loop region was amplified from 365 dogs and determined for mtDNA sequence. The diversity of the 600bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV.

从绳纹、弥生、古坟、鄂霍次克和镰仓时期的145个古代犬标本中，测定了日本古代犬的线粒体DNA（mtDNA）序列。从独立制备的DNA提取物中扩增了线粒体控制区（198 bp）的高度可变序列至少两次，并将来自古代样本的序列与来自178只现代狗的单倍型进行了比较。从74只古代犬中扩增出198 bp的线粒体DNA片段，将其分为19种单倍型，其中包括5种现代犬的单倍型（M1、M2、M5、M10和M11）。三个主要的集群（CL 1至CL 3），区分在现代狗控制区使用系统发育分析，所有古代狗属于CL 1集群。北海道犬的起源被认为是来自南方犬种群，而不是来自北方犬种群。冲绳的古代犬是通过贸易活动从欧亚大陆传入的，而九宿时代的犬与现代琉球犬具有相同的单倍型。对日本狼的mtDNA进行了测定，探讨了日本狼与狗、狼的亲缘关系。为了进一步完善犬mtDNA数据库，对365只犬的mtDNA D-loop区进行了扩增和序列测定。600 bp序列的多样性分为64个单倍型，其中46个为新发现的单倍型，并将单倍型分为I ~ IV 4个簇。

项目成果

Yasuko Morii: "Ancient DNA reveals genetic lineage of Sus scrofa among archaeological sites in Japan"Anthropol. Sci.. 110・3. 313-328 (2002)

森井靖子：“古代 DNA 揭示了日本考古遗址中野猪的遗传谱系”《人类学》110・3（2002 年）。

Takuma Watanobe: "Ancient mitochondrial DNA reveals the origin of Sus scrofa from Rebun Island, Japan"J. Mol. Evol.. 52. 281-289 (2001)

Takuma Watanobe：“古代线粒体 DNA 揭示了日本礼文岛野猪的起源”J.

Yasuko Morii: "Ancient DNA reveals genetic lineage of Sus scrofa among Archaeological sites in Japan"Anthropological Science. 110 (3). 313-328 (2002)

森井康子：“古代 DNA 揭示了日本考古遗址中野猪的遗传谱系”《人类学科学》。

Shoko Takahashi: "Lineage classification of canine inheritable disorders using mitochondrial DNA haplotupes"J. Vet. Med. Sci.. 64・3. 255-259 (2002)

Shoko Takahashi：“利用线粒体 DNA 单倍体进行犬类遗传性疾病的谱系分类”，医学杂志 64・3。

Takuma Watanobe: "Prehistoric introduction of domestic pigs onto the Okinawa Islands : ancient Mitochondrial DNA evidence"J. Mol. Evol.. 55. 222-231 (2002)

Takuma Watanobe：“史前家猪引入冲绳群岛：古代线粒体 DNA 证据”J.