STUDY ON THE NERVE-IMMUNE SYSTEMS AND CELLULAR FACTORS INFLUENCE TO THE PATHOGENICTTY AND DISTRIBUTION OF PRP^<Sc>IN ANIMAL PRION DISEASES

动物朊病毒疾病中神经免疫系统和细胞因素对PRP^<Sc>致病性和分布影响的研究

• 批准号: 17380180
• 负责人: ISHIGURO Naotaka
• 金额: $ 9.07万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380180/
• 关键词: Prion; Immunology; Nerve; Transport; Mouse; BSE; Scrapie; Ileum; 体内伝播; 回腸遠位部

The pathogenesis of animal prion diseases is influenced by prion strain, genetic factors, natural immunology and several cellular factors in the affected hosts. In this study, genetic factors, nerve-immune system and uptake of PrP^<Sc> by macrophages were mainly studied. The following results were obtained.1. Polymorpbisms of PrP gene: Polymorphisms of the 863 cattle, 118 caprine and 136 sika dear (Cervus Nippon) PrP genes in Japan were examined. Several single-nucleotide polymorphisms (SNPs) were found in cattle and sika dear, but not find any amino acid substitutions. Two polymorphisms associated with the resistance to scrapie were found in 118 caprine.2. Bovine and sheep nerve systems: To determine the location, namber and segmental distribution of sympathetic, parasympathetic and sensory neurons innervating the ileum in cattle and sheep, tracer experiments with horseradish peroxidase were conducted. The labeled neurons were detected in the celiac and cranial mesenteric ganglion complex in both cattle and sheep. Only a few labeled neurons existed in the caudal mesenteric ganglion and the paravertebral ganglia.3. Uptake PrP^<Sc> by immune cells: To clarify the uptake the PrP^<Sc> by immune cells, the involvement of bovine macrophage in the early stage of prion infection was studied. Brain homogenates of scrapie and BSE were degraded sequentially in bovine macrophage and bovine peripheral immune cells.A part of this research has been published in the papers, the other parts are now prepared in the papers.

动物普恩病毒病的发病机制受病毒株系、遗传因素、自然免疫学和宿主多种细胞因素的影响。本研究主要从遗传因素、神经免疫系统和巨噬细胞摄取PrP^&lt；Sc&gt；取得了以下研究成果：1.PrP基因的多态性：检测了日本863头牛、118头山羊和136头梅花鹿PrP基因的多态性。在牛和梅花鹿中发现了几个单核苷酸多态(SNPs)，但没有发现任何氨基酸替代。在118头山羊中发现了两个与羊痒病抗性相关的基因多态性。牛和羊的神经系统：用辣根过氧化物酶进行示踪实验，以确定支配牛和羊回肠的交感、副交感和感觉神经元的位置、数量和节段性分布。在牛和羊的腹部和颅肠系膜神经节复合体中均可检测到标记神经元。尾侧肠系膜神经节和椎旁神经节中仅有少量标记神经元。免疫细胞摄取PrP^&lt；Sc&gt；：为了阐明免疫细胞摄取PrP^&lt；Sc&gt；，我们研究了牛巨噬细胞在PrP感染早期阶段的参与。羊痒病和疯牛病的脑匀浆在牛巨噬细胞和牛外周免疫细胞中依次降解，这项研究的一部分已经发表在论文中，其他部分正在准备中。

项目成果

ウシ回腸での物質の取り込み機構

牛回肠物质摄取机制

• 发表时间: 2008
• 作者: 石黒直隆;ら
• 通讯作者: ら

Uptake system in bovine ileum considering prion infections

考虑朊病毒感染的牛回肠摄取系统

• 发表时间: 2007
• 作者: Lwine;Sein;Yukiko;Sassa;Naotaka;Ishiguro;Yasuo;Inoshima;Hiroshi;Ueno;Yasushige Ohmori
• 通讯作者: Yasushige Ohmori

Characterization of anti-chiken PrP antibodies

抗鸡 PrP 抗体的表征

• 发表时间: 2006
• 作者: Naotaka;Ishiguro;Yasuo;Inoshima;Yukiko;Sassa;Shinichi;Nakashima
• 通讯作者: Nakashima

Localization of foreign nerves cells controlling bovine ileum nerves

控制牛回肠神经的外来神经细胞的定位

• 发表时间: 2009
• 作者: Yasushige;Ohmori;Yasurou;Atoji;Shouichiro;Saito;Hirosi;Ueno;Yasuo;Inoshima;Naotaka;Ishiguro
• 通讯作者: Ishiguro

ウシとマウスの免疫系細胞による異常型プリオン蛋白質の取り込みと分解能

牛和小鼠免疫系统细胞对异常朊病毒蛋白的摄取和解析

• 发表时间: 2006
• 作者: 佐々悠木子;ら
• 通讯作者: ら