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Systematic analysis of the neural circuits connecting the lower-order sensory neuropils and higher-order integrative regions of the Drosophila brain

连接果蝇大脑低阶感觉神经元和高阶整合区域的神经回路的系统分析

基本信息

批准号：
12480205
负责人：
ITO Kei
金额：
$ 6.78万
依托单位：
The University of Tokyo (2002)Okazaki National Research Institutes (2000-2001)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

In insects, visual information is processed in the optic lobe and conveyed to the central brain. Although neural circuits within the optic lobe have been studied extensively, relatively little is known about the connection between the optic lobe and the central brain. To understand how visual information is read by the neurons of the central brain, and what kind of centrifugal neurons send the control signal from the central brain to the optic lobe, we performed a systematic analysis of the visual projection neurons that connect the optic lobe and the central brain of Drosophila melanogaster. By screening about 4,000 GAL4 enhancer-trap strains, we identified 44 pathways. The overall morphology and the direction of information of each pathway were investigated by expressing cytoplasmic and presynapsis-targeted fluorescent reporters. A canonical nomenclature system was introduced to describe the area of projection in the central brain. We especially concentrated on the 14 visual projection neurons arising specifically from the lobula. Eight pathways form columnar arborization in the lobula, whereas the remaining six form tangential or tree-like arborization. Eleven are centripetal pathways, among which nine terminate in the ventrolateral protocerebrum. Terminals of each columnar pathway form glomerulus-like structure in different areas of the ventrolateral protocerebrum. The posterior lateral protocerebrum and the optic tubercle were each contributed by a single centripetal pathway. Another pathway connects the lobula on each side of the brain. Two centrifugal pathways convey signals from the posterior lateral protocerebrum to the lobula.

在昆虫中，视觉信息在视叶中处理并传递到中央大脑。尽管人们对视叶内的神经回路进行了广泛的研究，但对视叶与中央大脑之间的联系知之甚少。为了了解视觉信息是如何被中脑的神经元读取的，以及什么样的离心神经元将控制信号从中脑发送到视叶，我们对黑腹果蝇连接视叶和中脑的视觉投射神经元进行了系统的分析。通过筛选约4000株GAL4增强诱捕菌株，我们确定了44条通路。通过表达细胞质和突触前靶向荧光报告基因来研究各通路的整体形态和信息方向。介绍了一种规范的命名系统来描述中央脑的投影区域。我们特别关注了14个视觉投射神经元，这些神经元是专门从小叶中产生的。8条通路在小叶中形成柱状乔木，而其余6条形成切向或树形乔木。11条向心通路，其中9条终止于前大脑腹外侧。各柱状通路的末端在前脑腹外侧不同区域形成肾小球样结构。脑后外侧原脑和视神经结节均由一条向心通路组成。另一条通路连接着大脑两侧的小叶。两条离心通路将信号从前脑后部外侧传递到小叶。