Immunohistochemical Study of the Effect on Motor Cortex Stimulation for Deafferentation Pain; Comparison of c-Fos Expression Sites

运动皮质刺激对传入神经阻滞疼痛影响的免疫组织化学研究；

• 批准号: 13470281
• 负责人: TAKAHASHI Toshio
• 金额: $ 2.37万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470281/
• 关键词: Intractable pain; Deafferentation pain; Trigeminal nerve; Motor cortex electrical stimulation; c-fos; Cat; trigeminal vnerve; 求心路遮断痛; 中枢性疼痛; 頑痛症; 脳脊髄電気刺激療法

The effect of motor cortex stimulation therapy (MCS) for deafferentation pain evaluated on the basis of c-fos gene, a known pain marker. [Materials] Fifteen mature cats, weighing 1.5-3.5 kilograms were used. [Methods] The cats were divided into three groups: a deafferentation pain group in which the trigeminal ganglion was destroyed, an MCS group in which MCS was used after destruction of the trigeminal ganglion, and a control group. Sites and levels of c-fos expression were examined immunohistochemically. [Result] The percentage of c-Fos-positive cells in the spinal nucleus of the trigeminus increased in both the deafferentation pain and MCS groups, however, the increase was smaller in the left side of the brain in the MCS group. In the cingulate gyrus, the percentage of c-Fos-positive cells increased bilaterally in the deafferentation pain group, but the increase was greater in the MCS group. Although the percentage of c-Fos-positive cells in the insula and the parietal operculum increased bilaterally in the deafferentation pain and MCS groups, there were no statistically significant differences between these groups. In the pontine gray and periaqueductal gray matter, the percentage of c-Fos-positive cells in the deafferentation pain group increased bilaterally. There was no significant difference between the MCS group and the control group. [Conclusion] The cingulate gyrus, insula, and pariental operculum are activated after deafferentation. This activation is related to the development of deafferentation pain. Pain relief due to MCS is not dependent on the descending analgesic mechanism of the brain stem. Activation of the cingulate gyrus appears to be a factor in the analgesic mechanism of MCS.

以已知的疼痛标志物c-fos基因为基础，评价运动皮层刺激疗法（MCS）对去传入痛的疗效。[材料]成年猫15只，体重1.5- 3.5kg。[方法]将猫分为3组：切断三叉神经节的去传入痛组、切断三叉神经节后应用MCS的MCS组和对照组。免疫组化检测c-fos表达的部位和水平。[结果]传入阻滞痛组和MCS组三叉神经脊核内c-Fos阳性细胞百分比均增加，但MCS组左侧增加幅度较小。在扣带回中，c-Fos阳性细胞的百分比在去传入痛组双侧增加，但MCS组的增加更大。虽然c-Fos阳性细胞的百分比在小脑和顶叶盖双侧增加的传入神经阻滞疼痛和MCS组，有这些组之间没有统计学上的显着差异。在脑桥灰质和导水管周围灰质中，c-Fos阳性细胞的百分比在传入阻滞疼痛组双侧增加。MCS组与对照组之间无显著差异。[结论]扣带回、小脑顶盖和小脑顶盖在传入神经阻滞后被激活。这种激活与传入阻滞疼痛的发展有关。MCS引起的疼痛缓解不依赖于脑干的下行镇痛机制。扣带回的激活似乎是MCS镇痛机制中的一个因素。