Research on the manifestation mechanism of Parkinsonian tremor : On the role of serotonergic neuron of raphe nucleus.

目的：帕金森病的主要损害是中脑黑核(氯胺能神经元)和蓝斑(去甲肾上腺素能神经元)的选择性神经元死亡。然而，我们不能仅仅用这些损伤部位来解释静止性震颤的所有表现和机制。腹内侧被盖(VMT)被称为包括猴的帕金森震颤的区域。我们曾报道在猴和猫的VMT内局部注射辣根过氧化物酶标记了黑质和中缝背核的大量细胞。本研究的目的是阐明震颤的表现不仅与黑核的损伤有关，而且与中缝核5-羟色胺能神经元的损伤有关。方法：成年猫15只，随机分为中缝背核凝固组和非凝固组。电凝组黑质核损毁DO…用立体定向凝血技术破坏中缝背核后，MPTP可抑制更多的胺系统。两组均隔日肌肉注射MPTP(3 mg/kg/d)，共8~14次。然后记录注射后的反应并进行视频和肌电分析。在脑部连续切片样本中证实了凝固性病变。结果：两组患者MPTP治疗后虽然严重程度不同，但均有明显的僵硬表现。在中缝背核被准确地包括在凝固性病变中的三只猫，在重复注射MPTP第七次或第八次后观察到震颤。震颤发生在静息位和肢体轻微弯曲位，震颤周期与帕金森病震颤相似，频率为4~6赫兹。2只猫在注射MPTP后15~40min出现震颤，并逐渐消失。在另一只猫身上，在第八次注射MPTP后也观察到了类似的震颤，即使在没有注射MPTP的粘土上也是如此。在中缝背核没有被电凝准确破坏的猫中，震颤没有发生。结论：即使在猫身上，也有可能产生类似于帕金森氏症震颤的静止性震颤。由此推测，中缝核5-羟色胺系统和黑质核多巴胺系统的损害可能参与了帕金森震颤的发病机制。较少

PURPOSE: The main lesion of Parkinson's disease is the selective neuronal death of the nucleus niger (clopaminergic neuron) and locus ceruleus of the midbrain (noradrenergic neuron). However, we cannot necessarily explain all the manifestations and mechanism of resting tremor by these sites of damage alone. The ventromedial tegmentum (VMT) is known as the region that includes Parkins-onian tremor in the monkey. We have reported that great numbers of cells in the nucleus niger and dorsal raphe nucleus were labeled by the local injection of horseraclish peroxidase into VMT in both of monkeys and cats . It is the purpose of this research to clarify that damages or not only the nucleus niger but also raphe nucleus serotonergic neurons are involved in the manifestation of tremor. METHOD : Fifteen mature cats were divided into two groups : one is a coagulation group of the dorsal raphe nucleus, and the other is a non-coagulation group. In the coagulation group, damage of the nucleus niger do … More pamine system is induded by MPTP, after the destruction of the dorsal raphe nucleus using a stereotactic coagulation technique. In both groups, MPTP (3 mg/kg/day) was injected intramuscularly every other day 8〜14 times in total. The reactions after injection were then recorded and analyzed in video and electromyogram. The coagulated lesions were confirmed in the serial-section samples of the brain. RESULT : Though there was a difference of severity, rigidity was observed in both groups after MPTP administration. In the three cats in which the dorsal raphe nucleus was accurately included in the coagulated lesion, the tremor was observed after the seventh or eighth repetitive administration of MPTP. The tremor occurred in the resting position and lightly bending position of the limbs, and its cycle was similar to Parkinsonian tremor of 4〜6 Hz. In two cats, the tremor appeared throughout the 15〜40 minutes after MPTP administration, and gradually dissappeared. In one other cat, a similar tremor was observed after the eighth administration of MPTP, even on the clay when MPTP was not administered. In the cats of which the dorsal raphe nucleus was not destroyed accuratehr by coagulation, the tremor did not develop. CONCLUSION : Even in the cat, it was possible to create a resting tremor that is similar to Parkinsonian tremor. From the facts described above, we may conclude that damages both of the raphe nucleus serotonin system and the nucleus niger dopamine system may be involvecl in the mechanism for Parkinsonian tremor . Less