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Analyses of molecular mechanisms of sensory processing in C.elegans

线虫感觉加工的分子机制分析

基本信息

批准号：
14580688
负责人：
ISHIHARA Takeshi
金额：
$ 2.3万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Animals sense many environmental stimuli, and process these sensory information in the nervous system. This sensory processing includes processes for selection of appropriate information, proper integration, and modification by experience or memory. To elucidate molecular and neuronal mechanisms for sensory processing, we are studying mutants defective in sensory intimation and learning behavior.hen-1 mutants show defects in integration of two sensory signals Cu^<2+> and attractive odorants. hen-1 mutant animals slow weaker tendency to cross the Cu^<2+> barrier during migrating toward diacetyl, although they appear not to exhibit defects in chemotaxis to diacetyl nor avoidance of Cu^<2+>. Furthermore, hen-1 mutant animals show abnormality in behavioral plasticities induced by paired presentation of NaCl and starvation and of temperature and starvation. The hen-1 gene encodes a secretory protein with an LDLa motif which is most homologus to a Drosophila signaling molecule, Jeb. HEN-1 is expressed in ASE and AIY neurons and functions cell-non-autonomously in the mature nervous system.Recently, Jeb in Drosophila is reported to regulate development of mesodermal cells through a receptor tyrosine kinase, DAlk, which is a homologue of proto-oncogene, Alk. Since the LDLa domain in Jeb is similar to that in HEN-1, we started to analyze the scd-2 gene, which encodes a receptor tyrosine kinase similar to DAlk. scd-2 mutants show behavioral deflects same as hen-1 mutants in paradigms for sensory integration and learning.

动物感知许多环境刺激，并在神经系统中处理这些感觉信息。这种感觉加工包括选择适当的信息、适当的整合以及通过经验或记忆进行修正的过程。为了阐明感觉加工的分子和神经元机制，我们正在研究感觉暗示和学习行为缺陷的突变体。hen-1突变体表现出两种感官信号Cu^<2+>和吸引气味的整合缺陷。hen-1突变体动物在向二乙酰迁移过程中，穿越Cu^<2+>屏障的倾向较弱，尽管它们对二乙酰的趋化性没有表现出缺陷，也没有回避Cu^<2+>。此外，在NaCl和饥饿、温度和饥饿的双重作用下，hen-1突变体动物表现出异常的行为可塑性。hen-1基因编码一种具有LDLa基序的分泌蛋白，该蛋白与果蝇信号分子Jeb最为同源。HEN-1在ASE和AIY神经元中表达，在成熟的神经系统中发挥细胞非自主功能。最近，有报道称果蝇中的Jeb通过酪氨酸激酶受体DAlk调节中胚层细胞的发育，DAlk是原癌基因Alk的同源物。由于Jeb中的LDLa结构域与HEN-1中的相似，我们开始分析scd-2基因，该基因编码与DAlk相似的受体酪氨酸激酶。sd -2突变体在感觉统合和学习方面表现出与hen-1突变体相同的行为偏转。