A study of the overcoming the weak point of a nutritional supplementary food, Spirulina in nutrition

克服营养辅助食品螺旋藻营养不足的研究

• 批准号: 15580113
• 负责人: WATANABE Fumio
• 金额: $ 1.66万
• 依托单位: Kochi Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580113/
• 关键词: Nutritional supplementary food; Spirulina; Vitamin B_<12>; Corrinoid compounds; Methionine synthase; metH gene; Cyanobacteria; Coenzyme; コリノイド化合物; ビタミンB_12; コリノイド; コバルト元素; 微細藻類; 微生物育種; バイオマス

A nutritional supplementary food, Spirulina (Spirulina sp., a cyanobacterium), contains substantial amount of 7-adenynyl cobaminde (pseudovitamin B_<12>), which is inactive for humans.To overcome the weak point of the Spirulina food in nutrition, fundamental information on physiological function and biosynthesis of pseudovitamin B_<12> in the bacterial cells was studied.1. Physiological function of pseudovitamin B_<12>Spirulina platensis contains substantial amount of pseudovitamin B_<12> and high specific activity of vitamin B_<12>-dependent methionine synthase. Molecular cloning of the vitamin B_<12>-dependent methionine synthase gene form S. platensis was conducted. Spirulina genomic DNA was purified from the cell. The degenerate primers were designated from well-conserved sequences of the known other cyanobacterial MetH. Using the primers, purified DNA was amplified by PCR. An open reading frame is 3549bp. The deduced amino add sequence showed high similarity to Thichodesmium erythraeum EMS 101 MetH. The binding motif of vitamin B_<12> and s-adenosyl-L-methioneni are completely conserved. These results indicated that the open reading frame encodes a metH. Pseudovitamin B_<12> may function as the coenzyme of the methionine synthase in S. platensis.2. Effect of the addition of precursor of B_<12>When 5,6-dimethylbenzimidazol as a precursor of the lower nucleotide moiety of vitamin B_<12> was added to the medium in order to evaluate whether pseudovitamin B_<12> is converted to true vitamin B_<12> in the bacterial cells. The addition of the precursor inhibited significant the cell growth.

一种营养补充食品，螺旋藻（Spirulina sp.，本<12>研究针对螺旋藻食品营养学上的不足，对螺旋藻的生理功能及其生物合成的基础知识进行了<12>研究.伪维生素B_的生理功能<12>钝顶螺旋藻含有大量的伪维生素B_<12>和高比活的维生素B_<12>依赖的甲硫氨酸合成酶。维生素B_<12>依赖型甲硫氨酸合酶基因的克隆。进行Platensis。从细胞中纯化螺旋藻基因组DNA。简并引物从已知的其他蓝藻MetH的保守序列中指定。使用引物，通过PCR扩增纯化的DNA。开放阅读框为3549 bp。推导的氨基酸序列与Thichodesmium acetraeum EMS 101 MetH高度相似。维生素B_<12>和s-腺苷-L-甲硫氨酸的结合基序是完全保守的。这些结果表明开放阅读框架编码metH。假维生素B_<12>可能是S. platensis.2.添加维生素B_前体的影响<12>当5，6-二甲基苯并咪唑作为维生素B_的低级核苷酸部分的前体<12>添加到培养基中以评价假维生素B_是否在细菌细胞中<12>转化为真维生素B_<12>时。前体的加入显著抑制了细胞的生长。

项目成果

バクテリアとアーキアで異なるコバミド補酵素合成サルベージ経路の存在

细菌和古细菌中存在不同的钴酰胺辅酶合成补救途径

• 发表时间: 2005
• 期刊: ビタミン 79
• 作者: 高野 良;原 三郎 他32名;渡辺文雄
• 通讯作者: 渡辺文雄

Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti

苜蓿中华根瘤菌同型二聚体甲基丙二酰辅酶A变位酶的纯化和表征

• 发表时间: 2003
• 期刊: Archives of Microbiology 180
• 作者: Kato;Ryuichi;Kajiya;Katsuko;Tokumoto;Hiromi;Kumazawa;Shigenori;Nakayama;Tsutomu;Emi Miyamoto
• 通讯作者: Emi Miyamoto

Euglena gracilis Zの成育に伴うビタミンB_<12>依存性メチオニン合成酵素活性の変動

眼虫Z生长过程中维生素B_<12>依赖性蛋氨酸合酶活性的变化

• 发表时间: 2005
• 期刊: 高知女子大学紀要 生活学部編 54
• 作者: Anunciado;R.V.P.;Nishimura;M.;Mori;M.;Ishikawa;A.;Tanaka;S.;Horio;F.;Ohno;T.;Namikawa;T.;Shigenori Kumazawa;谷岡由梨
• 通讯作者: 谷岡由梨