Gene analysis and in vitro culture of the new human malaria parasites

利用从缅甸和印度尼西亚采集的新型疟疾疟原虫和卵形疟原虫野生分离株，分析了SSUrRNA、细胞色素b、半胱氨酸蛋白酶和卵母细胞表面抗原基因。此外，还对野生菌株进行了体外培养试验。(1)新型卵圆线虫具有3条不同的SSUrRNA基因序列，且均不同于经典型尼日利亚- 1的2条序列。(2)新型疟疾疟原虫和卵形疟原虫的半胱氨酸蛋白酶基因序列与经典型、乌干达-1型和尼日利亚-1型不同。(3)新P卵圆与尼日利亚- 1型卵母细胞表面抗原基因序列不同。(4)在细胞色素b基因分析中，新的卵圆疟原虫与哈丁疟原虫有不同的序列，而新的疟疾疟原虫与乌干达疟原虫-1也有不同，但两者之间没有差异。(5)利用含葡萄糖和20%人血清的富集培养基对这些新的人疟疾寄生虫进行体外培养。培养24-48小时后，它们生长到幼裂殖阶段，但未观察到进一步的生长。

Using wild isolates of the new types of P. malariae and P. ovale collected from Myanmar and Indonesia, SSUrRNA, cytochrome b, cysteine protease or ookinete surface antigen genes were analyzed. In addition, in vitro culture was examined using wild isolates.(1)The new type of P. ovale possessed three different sequences of SSUrRNA gene, and all of them were differed from two sequences of the classic type, Nigerian-I.(2)The new types of P malariae and P. ovale had different sequences of cysteine protease gene from each classic type, Uganda-1 or Nigerian-I.(3)The new P ovale had different sequences of ookinete surface antigen genes from Nigerian-I.(4)In analysis of cytochrome b gene, the new P. ovale had a different sequence from the Harding strain, whereas the new P. malariae were also different from Uganda-1, but there was no difference between the two new types.(5)In vitro cultures of these new human malaria parasites were performed, using an enriched medium with glucose and 20% human serum. After cultivation for 24-48 hrs, they grew to young schizont stages, but further growth was not observed.