Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

我们以前的研究结果表明，从缅甸和越南收集的恶性疟原虫野生分离株很容易适应体外培养，而那些从印度尼西亚岛屿收集的未能适应。这些结果可能表明，印尼野生菌株有一种倾向，不容易适应体外培养，不同于那些分布在大陆SEA。因此，本研究进行了确认这些以前的研究结果，并从大陆SEA或印度尼西亚岛屿收集的野生菌株之间的细胞学和分子流行病学比较。从印度尼西亚许多岛屿收集的所有20个野生分离株不能再次适应体外培养，所有寄生虫在培养7天内消失。另一方面，从越南和柬埔寨收集的7个野生菌株进行了适应体外培养。特别是，从越南收集的分离物生长良好，并形成许多年轻的配子体。通过降低培养基中的葡萄糖水平可以诱导该菌株大量产生配子体。利用该分离物和印度尼西亚分离物，通过Roche基因组测序仪FLX+系统（Pyrosequence方法）尝试在分子水平上进行比较，但尚未发现它们之间的任何差异。

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.