Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

东南亚大陆及岛屿地区恶性疟原虫的分子流行病学和细胞学研究

• 批准号: 22406011
• 负责人: KAWAMOTO Fumihiko
• 金额: $ 9.9万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22406011/
• 关键词: 国際研究者交流; インドネシア:ベトナム:カンボジア; 熱帯熱 マラリア原虫; 東南アジア; 分布領域; 分子疫学調査; 遺伝子解析; 人工 培養; インドネシア：ベトナム：タイ; 熱帯熱マラリア原虫; 人工培養; インドネシア:ミャンマー; インドネシア:パプアニューギニア

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.

我们以前的研究结果表明，从缅甸和越南收集的恶性疟原虫野生分离株很容易适应体外培养，而那些从印度尼西亚岛屿收集的未能适应。这些结果可能表明，印尼野生菌株有一种倾向，不容易适应体外培养，不同于那些分布在大陆SEA。因此，本研究进行了确认这些以前的研究结果，并从大陆SEA或印度尼西亚岛屿收集的野生菌株之间的细胞学和分子流行病学比较。从印度尼西亚许多岛屿收集的所有20个野生分离株不能再次适应体外培养，所有寄生虫在培养7天内消失。另一方面，从越南和柬埔寨收集的7个野生菌株进行了适应体外培养。特别是，从越南收集的分离物生长良好，并形成许多年轻的配子体。通过降低培养基中的葡萄糖水平可以诱导该菌株大量产生配子体。利用该分离物和印度尼西亚分离物，通过Roche基因组测序仪FLX+系统（Pyrosequence方法）尝试在分子水平上进行比较，但尚未发现它们之间的任何差异。

项目成果

Genotypes of Candida albicans involved in development of candidiasis and their distribution in oral cavity of non-candidiasis individuals

与念珠菌病发生相关的白色念珠菌基因型及其在非念珠菌病个体口腔中的分布

• 发表时间: 2011
• 期刊: Med. Mycol. J.
• 作者: Y. Takagi;H. Hattori;H. Adachi;S. Takakura;T. Horii;A. Chindamporn;H. Kitai;R. Tanaka;T. Yaguchi;H. Fukano;F. Kawamoto;K. Shimozato;T. Kanbe
• 通讯作者: T. Kanbe

Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax: prospects for genome-wide association studies.

• DOI: 10.1186/1471-2156-11-65
• 发表时间: 2010-07-13
• 期刊: BMC genetics
• 影响因子: 2.9
• 作者: Orjuela-Sánchez P;Karunaweera ND;da Silva-Nunes M;da Silva NS;Scopel KK;Gonçalves RM;Amaratunga C;Sá JM;Socheat D;Fairhust RM;Gunawardena S;Thavakodirasah T;Galapaththy GL;Abeysinghe R;Kawamoto F;Wirth DF;Ferreira MU
• 通讯作者: Ferreira MU

Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.

• DOI: 10.3314/jjmm.52.129
• 发表时间: 2011-01-01
• 期刊: Medical mycology journal
• 影响因子: 1
• 作者: Shimizu, Kazue;Hattori, Hisao;Kanbe, Toshio
• 通讯作者: Kanbe, Toshio

Molecular epidemiology of malaria and G6PD deficiency in SEA

东南亚疟疾和 G6PD 缺乏症的分子流行病学

• 发表时间: 2011
• 作者: 佐々木 裕;萩原 恭二;山田 和範;村上 知行;間 陽子;Iwamoto N;加藤光;F. Kawamoto
• 通讯作者: F. Kawamoto

Microsatellite-based genotyping of Candida albicans isolated from patien

基于微卫星的患者白色念珠菌基因分型

• 发表时间: 2011
• 期刊: Med.Mycology Japan
• 作者: K.Shimizu;et al
• 通讯作者: et al