Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

我们先前的研究结果表明，从缅甸和越南采集的恶性疟原虫野生分离株很容易适应体外培养，而从印度尼西亚岛屿采集的恶性疟原虫分离株则不能适应体外培养。这些结果可能表明印尼野生分离株不容易适应体外培养，不同于分布在内陆海的分离株。因此，这项研究是为了证实这些先前的发现，并对从大陆海或印度尼西亚岛屿收集的野生分离株进行细胞学和分子流行病学比较。从印尼许多岛屿收集的20个野生分离物都不能再适应体外培养，培养7天内所有寄生虫都消失了。另一方面，对从越南和柬埔寨采集的7个野生菌株进行了体外培养。特别是从越南采集的一个分离物生长良好，形成了许多年轻的配子体。这些菌株可以通过降低培养液中的葡萄糖水平来诱导配子体的大量产生。用该分离株与印度尼西亚分离株进行分子水平上的比较，用罗氏基因组测序仪Flx+系统进行比较，但没有发现差异。

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.