Surveys on new human malaria in South East Asia

东南亚新人类疟疾调查

• 批准号: 07041159
• 负责人: KAWAMOTO Fumihiko
• 金额: $ 4.74万
• 依托单位: Nagoya University School of medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07041159/
• 关键词: Malaria; Molecular diagnosis; Acridine orange staining; New variant; Thailand; Indonesia; Myanmar; China; 分子疫学; 熱帯熱マラリア; 四日熱マラリア; リボゾームRNA遺伝子

Field survey of malaria was conducted in Thailand, Indonesia, Myanmar and China using an acridine orange (AO) staining method. Diagnostic results were compared with those by microtiter plate hybridization (MPH), and a nested PCR methods.1. In Thailand, a total of 548 samples were obtained, and many P.malariae and P.ovale infections diagnosed by the AO method were confirmed by the MPH and/or nested PCR methods. Their prevalence reached about 20% and 2.2%, respectively. In addition, three samples of P.ovale infections were AO-positive but MPH-negative. Analysis of the target sequences revealed that these isolates were the same mutated type (two nucleotide deletion and one nucleotide substitution) with that found in Vietnam. On the other hand, P.falciparum isolates were found to possess a point mutation at the target region in the 18S rRNA gene.2. In China, many P.malariae infections were also detected from the Sichuan Province by the three methods. By the nested PCR method, a long deletion from amplified PCR product was found at the probe region, indicating that this may be a new mutated type of P.malariae. Later, the same mutated form was detected from Thailand, Indonesia, and Myanmar, indicating that it is widely distributing in SEA.3. In Myanmar, many P.malariae and P.ovale infections were also detected. The mutated types of both of P.malariae and P.ovale which had been found in Vietnam was also detected. In contrast, in Indonesia only six samples of P.malariae and only two of P.ovale were found. One sample of P.malariae was the same new type, but others were normal.

采用吖啶橙子（AO）染色法在泰国、印度尼西亚、缅甸和中国进行了疟疾现场调查。并与微量滴定板杂交（MPH）、巢式PCR等方法进行比较.在泰国，共获得548份样本，许多AO方法诊断的三日疟原虫和卵形疟原虫感染通过MPH和/或巢式PCR方法得到证实。其患病率分别达到20%和2.2%。此外，3份卵形疟原虫感染样本为AO阳性，但MPH阴性。靶序列分析显示，这些分离株与越南分离株的突变类型相同（两个核苷酸缺失和一个核苷酸替换）。另一方面，发现恶性疟原虫分离株在18 S rRNA基因的靶区域存在点突变.在我国四川省也发现了许多三日疟原虫感染病例。通过巢式PCR方法，在扩增的PCR产物中发现探针区域的长缺失，表明这可能是一种新的突变型疟疾疟原虫。后来，在泰国、印度尼西亚和缅甸检测到了相同的突变形式，表明它广泛分布于东南亚。在缅甸，也发现了许多三日疟原虫和卵形疟原虫感染。同时也检测到越南发现的三日疟原虫和卵形疟原虫的突变类型。相比之下，在印度尼西亚只发现了六个三日疟原虫样本和两个卵形疟原虫样本，其中一个三日疟原虫样本是同一个新类型，但其他样本都是正常的。

项目成果

F.Kawamoto: "Comparison of the morphologies of same malaria parasites stained with acridine orange and then Giemsa" J.Clinical Microbiology. (発表予定).

F.Kawamoto：“用吖啶橙和吉姆萨染色的相同疟疾寄生虫的形态比较”J.Clinical Microbiology（即将发表）。

M.U.Ferreira: "Stable patterns of alleic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam" J.Eukaryotic Microbiology. (発表予定).

M.U.Ferreira：“越南南部临床分离株恶性疟原虫裂殖子表面蛋白 1 的等位基因多样性的稳定模式”，J.Eukaryotic Microbiology（真核微生物学）。

M.Kimura et al. (in preparation): "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitology International.

M.木村等人。

M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam" J.Eukaryotic Microbiology. (in preparation).

M.U.Ferreira 等人：“越南南部临床分离株中恶性疟原虫裂殖子表面蛋白 1 等位基因多样性的稳定模式”J.Eukaryotic Microbiology。

Z.Mian et al.: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border" J.Clinical Microbiology. (in preparation).

Z.Mian 等人：“泰缅边境疟疾患者中三日疟原虫和卵形疟原虫的高流行”J.Clinical Microbiology。