Surveys on malaria and gene analysis in Southeast Asia

东南亚疟疾调查和基因分析

• 批准号: 11691205
• 负责人: KAWAMOTO Fumihiko
• 金额: $ 4.8万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691205/
• 关键词: malaria; Southeast Asia; Myanmar; G6PD deficiency; Plasmodium malariae; SSUrRNA gene; field survey; new type; 卵形マラリア; タイ; ベトナム; インドネシア; 新型四日熱マラリア原虫; リボゾームRNA遺伝子

During 1999-2000, field surveys of malaria and G6PD deficiency were conducted in Thailand, Indonesia, My anmar and Vietnam by using an acridine orange staining and a rapid diagnosis of G6PD deficiency.1. In Thailand, malaria and G6PD surveys were performed at Sangkhlaburiand Ranong. At Ranong, many infections with Plasmodium malariae and some with P.ovale were detected.2. In Vietnam, malaria and G6PD surveys were done at Bao Loc, Lam Dong Province, and numbers of malaria patients seemed to increase from before.3. In Indonesia, we surveyed malaria and G6PD at Maumere, Flores island, East Nusa Tenggara Province, and Banuwangi, Pachitan, Kangean island, East Jawa Province. At Banuwangi, all patients infected with P.falciparum possessed the same type of SERA antigen gene, indicating that falciparum malariamay be spread from one patient.4. In Myanmar, malaria and G6PD surveys were conducted in many villagesin Mon State, Shan State and Rakhine State. In all villages, we detected many infections with P.malariae and some with P.ovale. In addition, two new types of P.malariae were detected from many villages. Their morphologies were different from the typical P.malariae, having irregularshapes of early trophozoites and 2-3 chromatin granules. The 18S ribosomal RNA (SSUrRNA) gene was also different from the reported sequences of typical P.malariae, indicating that the two types may be new species in P.malariae group.

1999-2000年期间，在泰国、印度尼西亚、缅甸和越南进行了疟疾和G6PD缺乏症的实地调查，采用吖啶橙染色和G6PD缺乏症的快速诊断。在泰国，在sangkhlaburii和Ranong进行了疟疾和G6PD调查。在拉农，检出多例疟疾感染和部分卵形疟原虫感染。在越南，疟疾和G6PD调查在林同省保禄进行，疟疾患者的数量似乎比以前有所增加。在印度尼西亚，我们在东努沙登加拉省弗洛雷斯岛的毛梅里和东爪哇省坎吉安岛的巴努旺吉、帕奇坦、巴努旺吉调查了疟疾和G6PD。在Banuwangi，所有感染恶性疟原虫的患者都具有相同类型的血清抗原基因，这表明恶性疟疾可能由一名患者传播。在缅甸，在孟邦、掸邦和若开邦的许多村庄进行了疟疾和G6PD调查。在所有村庄，我们都检测到许多疟疾疟原虫感染和一些卵形疟原虫感染。此外，在许多村庄还检出了两种新的疟疾假体。它们的形态与典型的疟疾疟原虫不同，具有不规则形状的早期滋养体和2-3个染色质颗粒。18S核糖体RNA （SSUrRNA）基因序列也与文献报道的典型疟原虫不同，表明这两种疟原虫可能是疟原虫类群中的新种。

项目成果

Q.Liu et al.: "Sequence diversity of serine repeat antigen gene exon II of Plasmodium falciparum in worldwide collected wild isolates."Southeast A.J.Trop.Med.Pub.Health. 31(in press).

Q.Liu 等人：“全球收集的野生分离株中恶性疟原虫丝氨酸重复抗原基因外显子 II 的序列多样性。”Southeast A.J.Trop.Med.Pub.Health。

I.S.Tantular et al.: "Field survey of malaria at the Flores Island, East Nusa Tenggara Province, Indonesia, and detection of P. malariae and P. ovale using two PCR-based diagnoses."Folia Medica Indonesia. 35. 38-42 (1999)

I.S.Tantular 等人：“对印度尼西亚东努沙登加拉省弗洛雷斯岛的疟疾进行实地调查，并使用两种基于 PCR 的诊断方法检测三日疟原虫和卵形疟原虫。”Folia Medica Indonesia。

川本文彦、水野サホ子: "マラリア原虫を見落とすな-アクリジンオレンジ染色"臨床調査. 43. 1625-1629 (1999)

Fumihiko Kawamoto、Sahoko Mizuno：“不要忽视疟疾寄生虫 - 吖啶橙染色”临床研究。 43. 1625-1629 (1999)。

Tantular, I.S. et al.: "Field trials of a rapid test for G6PD deficiency in combination with a rapid diagnosis of malaria."Trop. Med. & Int. Health. 4. 245-250 (1999)

坦图拉，I.S.

A.Laoboonchai et al.: "PCR-based ELISA technique for malaria diagnosis of specimens from Thailand."Trop.Med.& Int.Health. (in press).

A.Laoboonchai 等人：“基于 PCR 的 ELISA 技术，用于泰国标本的疟疾诊断。”Trop.Med。