Molecular epidemiological study on the variant type of Plasmodium falciparum and trial for in vitro culture of gametocytes

恶性疟原虫变异型的分子流行病学研究及配子体体外培养试验

• 批准号: 19406012
• 负责人: KAWAMOTO Fumihiko
• 金额: $ 9.98万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19406012/
• 关键词: 国際研究者交流; インドネシア:ベトナム・カンボジア・タイ; 熱帯熱マラリア; 新型原虫; 分子疫学調査; ヒトマラリア原虫; 遺伝子解析; 生殖母体培養; インドネシア:ベトナム; インドネシア:カンボジア:ベトナム; インドネシア:ベトナム:カンボジア:タイ

During epidemiological study on malaria from 2005 to 2006, Plasmodium falciparum (Pf) with very thin morphology of gametocytes was found in 4 patients in Cambodia and Indonesia. Analysis of 18S ribosomal RNA gene indicated that they are belonging to the variant type of Pf detected from Vietnam, and they had the same sequence variation at the target region for molecular diagnosis. Therefore, in this study, we continued similar epidemiological study on the variant Pf whether the strange shape of gametocytes belongs to the variant Pf or not by analyzing the 18S ribosomal RNA gene. In addition, our study had another aim ; a trial to cultivate in vitro from gametocytes to sporozoites in the variant or ordinary Pf (also gametocytes of Plasmdium vivax ; Pv) at the field.We surveyed malaria in Indonesia, Cambodia, Vietnam and Thailand from April 2007. In 2007, we found 2 patients infected with the variant Pf in Cambodia, but these bloods did not contain gametocytes. In addition, we detected 1 patient infected with Pv gametocytes, but trial of in vitro culture was failed. In 2008, we found 2 patients infected with gametocytes of the ordinary Pf, and tried in vitro culture of gametocytes. Furthermore, we detected 1 patient infected with Pv gametocytes in Cambodia. However, all these trials of in vitro culture were also failed. In 2009, no patient infected with the variant Pf was found in Indonesia and Vietnam. Therefore, it is still not clear whether morphology of gametocytes is different or not between the ordinary Pf and the variant Pf.

在2005 - 2006年的疟疾流行病学研究中，在柬埔寨和印度尼西亚的4名患者中发现了配子体形态非常薄的恶性疟原虫（Pf）。18 S核糖体RNA基因分析表明，它们均属于越南产Pf的变异型，且在分子诊断的靶区存在相同的序列变异。因此，本研究通过对18 S核糖体RNA基因的分析，继续对Pf变异体进行类似的流行病学研究，以确定配子体的奇怪形状是否属于Pf变异体。此外，我们的研究还有另一个目的;一个试验，培养从配子体到子孢子的变种或普通的Pf（也配子体的间日疟原虫; Pv）在field.We调查疟疾在印度尼西亚，柬埔寨，越南和泰国从2007年4月。2007年，我们在柬埔寨发现了2例感染Pf变体的患者，但这些血液不含配子体。此外，我们还检测到1例患者感染了Pv配子体，但体外培养试验失败。2008年，我们发现2例普通型Pf配子体感染患者，并进行了配子体体外培养的尝试。此外，我们在柬埔寨检测到1例Pv配子体感染患者。然而，所有这些体外培养试验也都失败了。2009年，印度尼西亚和越南没有发现感染Pf变种的患者。因此，尚不清楚普通Pf和变异Pf之间配子体的形态是否不同。

项目成果

Molecular epidemiology on malaria and G6PD deficiency in SEA

东南亚疟疾和 G6PD 缺乏症的分子流行病学

• 发表时间: 2012
• 作者: Tanabe K;Arisue N;Palacpac NMQ;Yagi M;Tougan T;Honma H;Ferreira MU;Farnert A;Bjorkman A;Kaneko A;Nakamura M;Hirayama K;Mita T;Horii T;I.S. Tantular & F. Kawamoto
• 通讯作者: I.S. Tantular & F. Kawamoto

Improvement of the repetitive sequence (RPS)-based identification and genotyping of Candida albicans using ALT-specific primers

使用 ALT 特异性引物改进基于重复序列 (RPS) 的白色念珠菌鉴定和基因分型

• 发表时间: 2009
• 期刊: Jpn. J. Infect. Dis. 62
• 作者: H. Hattori;F. Kawamoto;T. Kanbe;他3名
• 通讯作者: 他3名