Surveys on new human malaria in South East Asia
Surveys on new human malaria in South East Asia
批准号:
09041179
负责人:
KAWAMOTO Fumihiko
金额:
$4.61万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for international Scientific Research
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998
中文摘要
1.在1997-1998年期间,利用吖啶橙子染色法在泰国、印度尼西亚、缅甸、老挝、越南和中国进行了疟疾的实地调查。在这些国家共对约13000名志愿者进行了检查,并在约2000年发现了疟疾患者。对部分阳性标本进一步进行了PCR诊断,并在中国和东南亚地区发现了一种新的三日疟原虫变异株。该变异体在核糖体RNA基因中具有突变序列,在靶区域缺失19-bp。新变异体的靶序列前半部分与间日疟原虫和诺氏疟原虫的靶序列相同,后半部分与疟疾疟原虫的靶序列一致。由于这种变异型在非洲没有发现,因此很可能这种变异型只分布于东亚。所有感染该变异型的患者均与其他疟疾种混合感染。为了进一步的分子和临床研究,找到单独感染这种变异形式的患者是必要的。还进行了G6 PD缺乏症快速检测的现场试验,并进行了疟疾的现场快速诊断。通过使用这两种快速测试,伯氨喹是安全的处方疟疾患者感染间日疟原虫和恶性疟原虫配子体。
英文摘要
1. During 1997-1998, field surveys of malaria were conducted in Thailand, Indonesia, Myanmar, Laos, Vietnam and China by using an acridine orange staining. A total of about 13000 volunteers were examined in these countries, and malaria patients were detected in about 2000.2. PCR-based diagnoses were further examined in a part of malaria-positive samples, and a new variant form of P.malariae was detected from China and Southeast Asia. This variant form possessed mutated sequence in the ribosomal RNA gene with 19-bp deletion at the target region.3. The anterior part in the target sequence of the new variant form was the same with those of P.vivax and P.knowlesi, while its posterior part was corresponding to that of P.malariae. As this variant form was not found in Africa, it is probable that the variant form may distribute only in East Asia. All of patients infected with this variant form were mix-infected with other malaria species. For further studies on molecularly and clinically, it is essential to find patients infected singly with this variant form.4. Field trials of a rapid test for G6PD deficiency were also performed with a rapid diagnosis of malaria on-site. By using these two rapid tests, primaquine was prescribed safely to malaria patients infected with P.vivax and gametocytes of P.falciparum.
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川本 文彦: "アクリジンオレンジ染色によるマラリアの迅速診断法。" 臨床検査. 42. 243-247 (1998)
Fumihiko Kawamoto:“使用吖啶橙染色的疟疾快速诊断方法。”临床实验室。 42. 243-247 (1998)
DOI:
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作者:
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通讯作者:
I.S.Tantular et al.: "Field trials of a rapid test for G6PD deficiency in combination with a rapid diagnosisof malaria." Tropical Medicine & International Health. (In press). (1997)
I.S.Tantular 等人:“G6PD 缺乏症快速检测与疟疾快速诊断相结合的现场试验。”
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通讯作者:
Q.Liu et al.: "Sequence variation in small subunit ribosomal RNA gene of Plasmodium malariae and its local prevalence in Sichuan, China." J.Clin.Microbiology. 36. 3378-3381 (1998)
Q.Liu 等人:“三日疟原虫小亚基核糖体 RNA 基因的序列变异及其在中国四川的当地流行情况。”
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作者:
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通讯作者:
M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from southwestern Brazilian Amazon" Am.J.Tropical Medicine & Hygiene. 59. 474-480 (1998)
M.U.Ferreira 等人:“巴西亚马逊西南部临床分离株中恶性疟原虫裂殖子表面蛋白 1 位点的等位基因多样性”Am.J.Tropical Medicine
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作者:
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通讯作者:
M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern" J.Eukaryotic Microbiology. 45. 131-136 (1998)
M.U.Ferreira 等人:“南方临床分离株中恶性疟原虫裂殖子表面蛋白 1 等位基因多样性的稳定模式”J.Eukaryotic Microbiology。
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共 18 条
Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas
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批准号:22406011
-
项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.9万
-
财政年份:2010
-
负责人:KAWAMOTO Fumihiko
-
依托单位:
Molecular epidemiological study on the variant type of Plasmodium falciparum and trial for in vitro culture of gametocytes
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批准号:19406012
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.98万
-
财政年份:2007
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负责人:KAWAMOTO Fumihiko
-
依托单位:
Molecular epidemiological study on the SERA antigen gene in Plasmodium falciparum
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批准号:17406010
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项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$7.23万
-
财政年份:2005
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负责人:KAWAMOTO Fumihiko
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依托单位:
Molecular epidemiology on distribution of new human malaria parasite and G6PD deficiency
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批准号:15406014
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.34万
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财政年份:2003
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负责人:KAWAMOTO Fumihiko
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依托单位:
Gene analysis and in vitro culture of the new human malaria parasites
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批准号:14570213
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.18万
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财政年份:2002
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负责人:KAWAMOTO Fumihiko
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依托单位:
Molecular epidemiology on two new human malaria in Southeast Asia
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批准号:13576006
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.21万
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财政年份:2001
-
负责人:KAWAMOTO Fumihiko
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依托单位:
Molecular and cellbiological studies on gametocytogenesis of a malaria parasite, Plasmodium falciparum
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批准号:12670230
-
项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.18万
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财政年份:2000
-
负责人:KAWAMOTO Fumihiko
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依托单位:
Surveys on malaria and gene analysis in Southeast Asia
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批准号:11691205
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$4.8万
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财政年份:1999
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负责人:KAWAMOTO Fumihiko
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依托单位:
Surveys on new human malaria in South East Asia
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批准号:07041159
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$4.74万
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财政年份:1995
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负责人:KAWAMOTO Fumihiko
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依托单位:
Studies on anti-malarial activity of gramicidin
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批准号:04670227
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1992
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负责人:KAWAMOTO Fumihiko
-
依托单位:
Studies on development of the sexual stages in Plasmodium
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批准号:01570212
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1989
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负责人:KAWAMOTO Fumihiko
-
依托单位:
Studies on the encystation of Entamoeba
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批准号:61570192
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1986
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负责人:KAWAMOTO Fumihiko
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依托单位:
海外基金