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Circadian and seasonal changes in human plasma orexin-A and serum melatonin

人血浆食欲素-A和血清褪黑激素的昼夜节律和季节变化

基本信息

批准号：
14570914
负责人：
USUI Akira
金额：
$ 2.18万
依托单位：
University of Yamanashi (2003)山梨医科大学 (2002)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570914/
关键词：
orexin melatonin sleep activity body temperature mood circadian seasonal 季節性変動

项目摘要

1. Sleep onset, offset and serum melatoninSubjects were eight healthy young adults (mean age 24.8 years) who lived around Kofu, Japan, Written informed consent was obtained from all the subjects. Kofu is located at 35°40' N and 138°34'E, and sunrise and sunset times in the summer and winter differ by about 2 h and 20 min. In Japan daylight-saving time has not been introduced. The subjects filled out a sleep log for one month in both the summer and winter, and the sleep-wake state was scored in 10-minute epochs. Based on the sleep log, daily sleep onset and offset times were looked for, and individual monthly averages were calculated. The subjects stayed in the experimental facility for 24 h in both the summer and winter. Illumination in the facility was less than 15 1x. Blood was sampled through an indwelling catheter in the forearm vein at 1-h to 3-h intervals. Serum melatonin was assayed by RIA. The phase of maximum of fitted three-harmonic regression model (3HMAX) was calculated. Sleep onset times were 0141 h in the summer and 0200 h in the winter (statistically n. s.), and sleep offset times were 0751 h and 0824 h, respectively. 3HMAX were 0258 h in the summer and 0406 h in the winter.2. Orexin-AWritten informed consent was obtained from all the subjects. Five subjects (26.0 years) stayed in the experimental facility for 24 h in both the summer and winter. Blood was sampled at 1-h intervals. Plasma orexin-A was assayed by RIA. Plasma orexin-A showed no circadian variation in both the summer and winter. But orexin-A in the summer (mean value 47.1 pg/ml) was higher than that in the winter (21.4 pg/ml).

1.研究对象为居住在日本甲府地区的8名健康年轻人（平均年龄24.8岁）。甲府位于北纬35°40'东经138° 34'，夏季和冬季的日出和日落时间相差约2小时20分钟。受试者在夏季和冬季都填写了一个月的睡眠日志，睡眠-觉醒状态以10分钟为单位进行评分。根据睡眠日志，寻找每日睡眠开始和偏移时间，并计算个人每月平均值。受试者在夏季和冬季都在实验设施中停留24 h。设施中的照度小于15 1x。通过前臂静脉留置导管以1-h至3-h的间隔采集血液。采用放射免疫法测定血清褪黑素。计算拟合的三次谐波回归模型（3 HMAX）的最大值的相位。睡眠起始时间夏季为0141 h，冬季为0200 h（统计学n。s.），睡眠偏移时间分别为0751 h和0824 h。3 HMAX夏季为0258 h，冬季为0406 h.获得所有受试者的Orexin-AWritten知情同意书。5名受试者（26.0岁）在夏季和冬季均在实验设施中停留24 h。每隔1小时采集一次血样。放免法测定血浆食欲素A含量。血浆食欲素-A在夏季和冬季均无昼夜节律变化。但夏季食欲素A（平均值47.1pg/ml）高于冬季（21.4pg/ml）。