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Clinical application of Cartilage-derived Retinoic Acid-sensitive Protein in Orthopaedic Surgery

软骨源性视黄酸敏感蛋白在骨科手术中的临床应用

基本信息

批准号：
14571370
负责人：
KONDO Seiji
金额：
$ 2.24万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

The concentrations in synovial fluid from osteoarthritis (CA) and rheumatoid arthritis (RA) were measured. The mean SF concentration of CD-RAP was significantly higher in CA than in RA. The CD-RAP in the mild CA group was significantly higher than in the moderate or severe CA groups. CD-RAP in the mild RA group was also significantly higher than in the other RA groups and decreased with disease progression. Immunohistochemical study showed that CD-RAP expression was observed in the cytoplasm of chondrocytes in newly formed fibrocartilage. As CD-RAP is mainly produced in young and proliferating chondrocytes, these results suggest that the level of CD-RAP in SF reflects remodeling of articular cartilage and has a possibility of a marker to objectively estimate the restorative reaction of chondrocytes. In addition, the concentration of CD-RAP in synovial fluid from revision surgery of hip arthroplasty was very low. This result supports specificity of CD-RAP to chondrocytes. because the cartilaginous tissue was excised at the previous surgery.Expression of CD-RAP was measured in cerebrospinal fluid from patients with spinal diseases. The concentrations of CD-RAP in cervical myelopathy, lumbar canal stenosis, and lumbar disc herniation were significantly higher than in the control group. The CD-RAP concentration was low in the control group, whereas it was significantly higher in spinal diseases that cause spinal stenosis. CD-RAP is expressed in cerebrospinal fluid as a result of damage to or stressing of neural structures and could be a marker for spinal diseases.The concentration of CD-RAP was analyzed using Sworm rat chondrosarcoma. The concentration increased with the progression of the tumor. When the tumor was removed, the consentration went tdown to normal range. The results show that CD-RAP could become a potential marker of chondrosarcoma.

测定骨关节炎（CA）和类风湿关节炎（RA）滑膜液中的浓度。CA组CD-RAP平均SF浓度显著高于RA组。轻度CA组CD-RAP明显高于中度和重度CA组。轻度RA组的CD-RAP也显著高于其他RA组，并随着疾病进展而降低。免疫组化研究显示，CD-RAP在新形成的纤维软骨软骨细胞细胞质中有表达。由于CD-RAP主要在年轻的增殖软骨细胞中产生，这些结果提示SF中CD-RAP的水平反映了关节软骨的重塑，有可能作为客观评价软骨细胞修复反应的标志物。此外，髋关节置换术翻修后的滑液中CD-RAP的浓度很低。这一结果支持CD-RAP对软骨细胞的特异性。因为软骨组织在之前的手术中被切除了。测定脊髓疾病患者脑脊液中CD-RAP的表达。CD-RAP在颈椎病、腰椎管狭窄和腰椎间盘突出症患者中的浓度明显高于对照组。CD-RAP浓度在对照组中较低，而在导致椎管狭窄的脊柱疾病中则明显较高。CD-RAP在脑脊液中表达是神经结构损伤或受压的结果，可能是脊柱疾病的标志。采用Sworm大鼠软骨肉瘤对CD-RAP进行浓度分析。浓度随着肿瘤的进展而增加。当肿瘤被切除后，浓度降至正常范围。结果表明CD-RAP可能成为软骨肉瘤的潜在标志物。