Clinical application of Caftilage-derived Retinoic Acid-sensitive Protein

为了探讨关节疾病与软骨源性视黄酸敏感蛋白（CD-RAP）的相关性，我们测定了骨关节炎（OA）和类风湿性关节炎（RA）患者滑液中的浓度。OA患者CD-RAP的平均SF浓度显著高于RA患者。轻度OA组CD-RAP明显高于中、重度OA组。轻度RA组的CD-RAP也显著高于其他RA组，并随疾病进展而下降。免疫组化结果显示，在新生纤维软骨中，CD-RAP表达于软骨细胞的胞浆中。由于CD-RAP主要在年轻和增殖的软骨细胞中产生，这些结果表明，SF中的CD-RAP水平反映了关节软骨的重塑，并有可能成为客观评价软骨细胞恢复反应的标志物。脊髓型颈椎病、腰椎管狭窄症和腰椎间盘突出症患者的CD-RAP浓度明显高于对照组。对照组的CD-RAP浓度较低，而导致椎管狭窄的脊柱疾病患者的CD-RAP浓度明显较高。为了研究CD-RAP在软骨肉瘤血清中的表达，我们测定了Sworm大鼠软骨肉瘤血清中CD-RAP的浓度。浓度随肿瘤进展而增加。当肿瘤被切除时，浓度下降到正常范围。结果表明，CD-RAP可能成为软骨肉瘤的潜在标志物。

To investigate the correlation between joint diseases and Cartilage-derived Retinoic Acid-sensitive Protein (CD-RAP), we measured the concentrations in synovial fluid- (SF) from osteoarthritis (OA) and rheumatoid arthritis (RA). The mean SF concentration of CD-RAP was significantly higher in OA than in RA. The CD-RAP in the mild OA group was significantly higher than in the moderate or severe OA groups. CD-RAP in the mild RA group was also significantly higher than in the other RA groups and decreased with disease progression. Immunohistochemical study showed that CD-RAP expression was observed in the cytoplasm of chondrocytes in newly formed fibrocartilage. As CD-RAP is mainly produced in young and proliferating chondrocytes, these results suggest that the level of CD-RAP in SF reflects remodeling of articular cartilage and has a possibility of a marker to objectively estimate the restorative reaction of chondrocytes.To quantify CD-RAP in human cerebrospinal fluid and to clarify its character, the expression was measured in cerebrospinal fluid from patients with spinal diseases. The concentrations of CD-RAP in cervical myelopathy, lumbar canal stenosis, and lumbar disc herniation were significantly higher than in the control group. The CD-RAP concentration was low in the control group, whereas it was significantly higher in spinal diseases that cause spinal stenosis. CD-RAP is expressed in cerebrospinal fluid as a result of damage to or stressing of neural structures and could be a marker for spinal diseases.To study the expression in serctm from chondrosarcoma, we measured CD-RAP concentration in serum of Sworm rat chondrosarcoma. The concentration increased with progression of the tumor. When the tumor was excised, the concentration went down to the normal range. The results show that CD-RAP could become a potential marker of chondrosarcoma.