Clinical application of Caftilage-derived Retinoic Acid-sensitive Protein

咖啡豆源性视黄酸敏感蛋白的临床应用

• 批准号: 12671408
• 负责人: KONDO Seiji
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671408/
• 关键词: CD-RAP; Synovial fluid; Chondrosarcoma; Cerebrospinal fluid; 変形性関節症; 慢性関節リウマチ; 脊柱管狭窄症

To investigate the correlation between joint diseases and Cartilage-derived Retinoic Acid-sensitive Protein (CD-RAP), we measured the concentrations in synovial fluid- (SF) from osteoarthritis (OA) and rheumatoid arthritis (RA). The mean SF concentration of CD-RAP was significantly higher in OA than in RA. The CD-RAP in the mild OA group was significantly higher than in the moderate or severe OA groups. CD-RAP in the mild RA group was also significantly higher than in the other RA groups and decreased with disease progression. Immunohistochemical study showed that CD-RAP expression was observed in the cytoplasm of chondrocytes in newly formed fibrocartilage. As CD-RAP is mainly produced in young and proliferating chondrocytes, these results suggest that the level of CD-RAP in SF reflects remodeling of articular cartilage and has a possibility of a marker to objectively estimate the restorative reaction of chondrocytes.To quantify CD-RAP in human cerebrospinal fluid and to clarify its character, the expression was measured in cerebrospinal fluid from patients with spinal diseases. The concentrations of CD-RAP in cervical myelopathy, lumbar canal stenosis, and lumbar disc herniation were significantly higher than in the control group. The CD-RAP concentration was low in the control group, whereas it was significantly higher in spinal diseases that cause spinal stenosis. CD-RAP is expressed in cerebrospinal fluid as a result of damage to or stressing of neural structures and could be a marker for spinal diseases.To study the expression in serctm from chondrosarcoma, we measured CD-RAP concentration in serum of Sworm rat chondrosarcoma. The concentration increased with progression of the tumor. When the tumor was excised, the concentration went down to the normal range. The results show that CD-RAP could become a potential marker of chondrosarcoma.

为了探讨关节疾病与软骨源性维甲酸敏感蛋白(CD-RAP)的相关性，我们测定了骨性关节炎(OA)和类风湿性关节炎(RA)滑液中CD-RAP的浓度。RA组CD-RAP平均SF浓度显著高于OA组。轻度骨性关节炎组的CD-RAP显著高于中、重度骨性关节炎组。轻度RA组CD-RAP也明显高于其他RA组，且随病情进展而降低。免疫组织化学研究显示，新生纤维软骨中CD-RAP表达位于软骨细胞胞浆中。由于CD-RAP主要在年轻和增殖的软骨细胞中产生，这些结果表明，SF中CD-RAP的水平反映了关节软骨的重塑，有可能成为客观评价软骨细胞修复反应的指标。脊髓型颈椎病、腰椎管狭窄症、腰椎间盘突出症患者CD-RAP浓度明显高于对照组。对照组CD-RAP浓度较低，而引起椎管狭窄的脊柱疾病患者CD-RAP浓度显著升高。CD-RAP在脑脊液中的表达是神经结构损伤或应激的结果，可作为脊柱疾病的标志物。为了研究CD-RAP在软骨肉瘤血清中的表达，我们测定了SWorm大鼠软骨肉瘤血清中CD-RAP的浓度。随着肿瘤的发展，血药浓度逐渐升高。肿瘤切除后，血药浓度降至正常范围。结果表明，CD-RAP有可能成为软骨肉瘤的潜在标志物。

项目成果

Yonekawa M: "Sarum Level of Cartilage-Derived Retinoic Acid-Sensitive Protein(CD-RAP) in Sworm Rat Chondrosarcoma"J Orthop Res.. (in press). (2001)

Yonekawa M：“蠕虫大鼠软骨肉瘤中软骨衍生的视黄酸敏感蛋白（CD-RAP）的沙鲁姆水平”J Orthop Res..（正在印刷中）。

Seiji Kondo: "Cytokine Regulation of Cartilage-Derived Retinoic Acid-Sensitive Protein(CD-RAP) in Primary Articular Chondrocytes : Supression by IL-1, bfGF, TGFband Stimulation by IGF-1"J Orthop Res.. 19(4). 712-719 (2001)

Seiji Kondo：“原代关节软骨细胞中软骨衍生视黄酸敏感蛋白 (CD-RAP) 的细胞因子调节：IL-1、bfGF、IGF-1 刺激 TGFband 的抑制”J Orthop Res.. 19(4)。

Naoki Natsume, Seiji Kondo Yukihiro Matsuyama, Kenji Sumida, Hidefumi Inou, Noriaki Kawakami, Linda, J Sandell and Hisashi Iwata: "Analysis of Cartilage-Derived Retinoic Acid-Sensitive Protein in Cerebrospinal Fluid from Patients with Spinal Diseases"Spin

Naoki Natsume、Seiji Kondo Yukihiro Matsuyama、Kenji Sumida、Hidefumi Inou、Noriaki Kawakami、Linda、J Sandell 和 Hisashi Iwata：“脊柱疾病患者脑脊液中软骨来源的视黄酸敏感蛋白的分析”Spin

Seiji Kondo: "Cytokine Regulation of Cartilage-Derived Retinoic Acid-Sensitive Protein(CD-RAP) in Primary Articular Chondrocytes : Supression by IL-1, bfGF, TGFband Stimulation by IGF-1"J Orthop Res. 19(4). 712-719 (2001)

Seiji Kondo：“原代关节软骨细胞中软骨衍生视黄酸敏感蛋白 (CD-RAP) 的细胞因子调节：IL-1、bfGF、IGF-1 刺激 TGFband 的抑制”J Orthop Res。

Seiji Kondo, Wei-Fen Xie, Linda J.: "Sandell Cytokine Regulation of Cartilage-Derived Retinoic Acid-Sensitive Protein(CD-RAP) in Primary Articular Chondrocytes : Suppression by IL-1, bFGF, TGF beta and stimulation by IGF-1 ""J Orthop Res. Jul. 19(4). 712-

Seiji Kondo、Wei-Fen Xie、Linda J.：“Sandell 细胞因子对原代关节软骨细胞中软骨来源的视黄酸敏感蛋白 (CD-RAP) 的调节：IL-1、bFGF、TGF β 的抑制和 IGF- 的刺激