Synthesis and properties of artificial nucleic acids utilizing pentaerythritol.

点击翻译按钮获取中文摘要

First, the synthesis and thermal stabilities of the triplexes containing either 2'-deoxyinosine (1) or 2'-deoxyxanthosine (3) in their second strands were examined. It was found that the triplexes with the 2'-deoxy-5-methylcytidine(dM)・1:dC and dM・1:dA base triplets are thermally stable, but those containing the dM・1:T and dM・1:dG base triplets are unstable under both neutral and slightly acidic conditions. On the other hand, it was found that the oligonucleotide containing 3 could form thermally stable triplexes with the oligonucleotides that involve four natural bases opposite the sites of 3. The rank of the thermal stabilities of the triplexes was as follows : the triplex containing the dM・3:dC base triplet > that containing the dM・3:dA base triplet > that containing the dM・3:T base triplet > that containing the dM・3:dG base triplet.Furthermore, the synthesis of triplexes possessing an anthraquinonyl group and composed of branched oligonucleotides (ONs) was examined. Binding ability of a triplex-binding protein (MBP-LOR3^<ARF>) to the triplexes was evaluated by an electrophoretic mobility shift assay (EMSA). It was found that the triplex, which has an antraquinonecarbonyl group at the 5'-end of the third strand and is connected with the pentaerythritol linker, has greater affinity to the protein than an unmodified triplex.