A Molecular Mechanism Coordinating Proliferation and Differentiation of Neural Precursor Cells

协调神经前体细胞增殖和分化的分子机制

• 批准号: 17500255
• 负责人: KAGAWA Tetsushi
• 金额: $ 2.24万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500255/
• 关键词: neural stem cells; cell proliferation; undifferentiated state; Wnt; FGF2; Notch1; GSK3beta; Hes1

The effects of Wnt signaling on neural progenitor cells were controversial: it was reported to either promote their cell proliferation or accelerate differentiation to become postmitotic neurons. To understand this discrepant role of the Wnt canonical pathway, we applied Wnt3a on the primary culture prepared from embryonic day 15 mouse hippocampus. Wnt3a increased the numbers of total cells, proliferating cells and differentiated neurons. This result phenomenologically suggests that Wnt3a induces both neural progenitor cell proliferation and neuronal differentiation. Notably, the ratio of the number of neurons to total cells after the culture was almost comparable. Wnt3a did not significantly affect the ratio of symmetric and asymmetric cell divisions, but accelerated cell cycle progression by shortening the cell cycle duration, which could result in increases of both proliferative cells and differentiated neurons.

Wnt信号对神经前体细胞的影响存在争议：据报道，它可以促进细胞增殖或加速分化为有丝分裂后的神经元。为了理解Wnt经典途径的这种差异性作用，我们将Wnt3a应用于从胚胎第15天小鼠海马制备的原代培养物。Wnt3a可增加总细胞数、增殖细胞数和分化神经元数。该结果在现象学上表明Wnt3a诱导神经祖细胞增殖和神经元分化。值得注意的是，培养后神经元数量与总细胞数量的比率几乎相当。Wnt3a对细胞对称分裂和不对称分裂的比例没有显著影响，但通过缩短细胞周期持续时间加速细胞周期进程，从而导致增殖细胞和分化神经元的增加。

项目成果

Activation of canonical Wnt pathway promotes proliferation of retinal stem cells derived from adult mouse ciliary margin

• DOI: 10.1634/stemcells.2005-0124
• 发表时间: 2006-01-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Inoue, T;Kagawa, T;Taga, T
• 通讯作者: Taga, T

bFGF endows dorsal telencephalic neural progenitors with ability to differentiate into oligodendrocytes but not g-aminobutyric acidergic neurons.

bFGF 赋予背侧端脑神经祖细胞分化为少突胶质细胞的能力，但不具有分化为 g-氨基丁酸能神经元的能力。

• 发表时间: 2006
• 期刊: J Neurosci Res. 83(5)
• 作者: Abematsu M.;et al.
• 通讯作者: et al.

Involvement of the Olig2 transcription factor in cholinergic neuron development of the basal forebrain

• DOI: 10.1016/j.ydbio.2006.01.031
• 发表时间: 2006-05-15
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Furusho, Miki;Ono, Katsuhiko;Ikenaka, Kazuhiro
• 通讯作者: Ikenaka, Kazuhiro

Wnt signaling controls the timing of oligodendrocyte development in the spinal cord

• DOI: 10.1016/j.ydbio.2005.03.020
• 发表时间: 2005-06-15
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Shimizu, T;Kagawa, T;Ikenaka, K
• 通讯作者: Ikenaka, K

Potentiation of astrogliogenesis by STAT3-mediated activation of BMP-Smad signaling in neural stem cells.

通过 STAT3 介导的神经干细胞中 BMP-Smad 信号激活增强星形胶质细胞生成。

• 发表时间: 2007
• 期刊: Molecular and Cellular Biology (印刷中)
• 作者: Sango;K.;et al.;(Maeda;N.;Ed.);Shinji Fukuda
• 通讯作者: Shinji Fukuda