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Analyses of disease resistance mechanism by rice acyltransferase family

水稻酰基转移酶家族抗病机制分析

基本信息

批准号：
17580087
负责人：
MORI Masaki
金额：
$ 2.18万
依托单位：
National Institute of Agrobiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580087/
关键词：
plant gene rice disease resistance acyltransferase 病害抵抗性アシルトランスフェラーゼ

项目摘要

OsAT1 is a rice acyltransferase gene isolated from the spotted leaf 18 mutant that was generated by activation tagging in rice. Rice plants overexpressing OsAT1 showed blast resistance. In this research, we also found that these plants have blight resistance. Although transcription of OsAT1 was not affected by blast infection in wild-type plants, we observed elevated transcript levels of two OsAT family members (OsAT15 and OsAT180). Transcription rates of the other eleven OsAT family members were not remarkably affected. OsAT15 was transcribed mainly in the young panicle, but it was not detected in the leaf blade, panicle and root of the wild-type plant. We generated rice plants overexpressing OsAT15 and performed blast resistance test. But they did not show blast resistance.We measured the fatty acid content of rice overexpressing OsAT1 to determine the biochemical function of OsATs. We found that the content of linoleic acid increased in these plants. The proteins coded by OsAT1 and OsAT15 were overproduced in E.coli with His-tag. The overproduced proteins were insoluble, so we are now trying to find ways to make them soluble. Simultaneously, we are making transgenic Arabidopsis overexpressing OsAT1 or OsAT15to for metabolome analysis.

OsAT 1是从水稻斑点叶18突变体中分离到的一个水稻酰基转移酶基因。过量表达OsAT1的水稻植株表现出抗稻瘟病的特性。在本研究中，我们还发现这些植物具有抗枯萎病的能力。虽然OsAT1的转录在野生型植物中不受稻瘟病感染的影响，但我们观察到两个OsAT家族成员（OsAT15和OsAT180）的转录水平升高。其他11个OsAT家族成员的转录率没有受到显着影响。OsAT15主要在幼穗中转录，而在野生型植株的叶片、穗和根中未检测到。我们获得了过量表达OsAT15的水稻植株，并进行了稻瘟病抗性测试。本研究通过测定过量表达OsAT1的水稻的脂肪酸含量来确定OsATs的生化功能。我们发现这些植物中亚油酸的含量增加。OsAT1和OsAT15编码的蛋白在带有His标签的大肠杆菌中过量表达。过量产生的蛋白质是不可溶的，因此我们现在正在努力寻找使它们可溶的方法。同时，我们正在制备过量表达OsAT1或OsAT15的转基因拟南芥，以进行代谢组学分析。