Analyses of disease resistance mechanism by rice acyltransferase family

水稻酰基转移酶家族抗病机制分析

基本信息

批准号：
17580087
负责人：
MORI Masaki
金额：
$ 2.18万
依托单位：
National Institute of Agrobiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580087/
关键词：
plant gene rice disease resistance acyltransferase 病害抵抗性アシルトランスフェラーゼ

项目摘要

OsAT1 is a rice acyltransferase gene isolated from the spotted leaf 18 mutant that was generated by activation tagging in rice. Rice plants overexpressing OsAT1 showed blast resistance. In this research, we also found that these plants have blight resistance. Although transcription of OsAT1 was not affected by blast infection in wild-type plants, we observed elevated transcript levels of two OsAT family members (OsAT15 and OsAT180). Transcription rates of the other eleven OsAT family members were not remarkably affected. OsAT15 was transcribed mainly in the young panicle, but it was not detected in the leaf blade, panicle and root of the wild-type plant. We generated rice plants overexpressing OsAT15 and performed blast resistance test. But they did not show blast resistance.We measured the fatty acid content of rice overexpressing OsAT1 to determine the biochemical function of OsATs. We found that the content of linoleic acid increased in these plants. The proteins coded by OsAT1 and OsAT15 were overproduced in E.coli with His-tag. The overproduced proteins were insoluble, so we are now trying to find ways to make them soluble. Simultaneously, we are making transgenic Arabidopsis overexpressing OsAT1 or OsAT15to for metabolome analysis.

OSAT1是一种从斑点的叶子18突变体中分离出来的水稻酰基转移酶基因，该基因是通过大米中的激活标记产生的。过表达OSAT1的水稻植物显示出抗爆炸性。在这项研究中，我们还发现这些植物具有抗枯萎病。尽管OSAT1的转录不受野生型植物的爆炸感染影响，但我们观察到两个OSAT家族成员的转录水平升高（OSAT15和OSAT180）。其他11个OSAT家族成员的转录率并未受到显着影响。 OSAT15主要在年轻的圆锥花序中转录，但在野生型植物的叶片，圆锥膜和根中未检测到它。我们产生了过表达OSAT15并进行抗爆炸性测试的水稻植物。但是它们没有显示出抗爆炸性。我们测量了过表达OSAT1的水稻的脂肪酸含量，以确定OSAT的生化功能。我们发现这些植物中亚油酸的含量增加。由OSAT1和OSAT15编码的蛋白质与His-Tag在E.Coli中过量生产。生产过量的蛋白质不太溶，因此我们现在正在尝试找到使它们溶解的方法。同时，我们正在制造过表达OSAT1或OSAT15TO的转基因拟南芥进行代谢组分析。